FLIT_ECOLI
ID FLIT_ECOLI Reviewed; 121 AA.
AC P0ABY2; P26610;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Flagellar protein FliT {ECO:0000255|HAMAP-Rule:MF_01180};
GN Name=fliT {ECO:0000255|HAMAP-Rule:MF_01180};
GN OrderedLocusNames=b1926, JW1911;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=JA11;
RX PubMed=1527488; DOI=10.1099/00221287-138-6-1051;
RA Kawagishi I., Mueller V., Williams A.W., Irikura V.M., Macnab R.M.;
RT "Subdivision of flagellar region III of the Escherichia coli and Salmonella
RT typhimurium chromosomes and identification of two additional flagellar
RT genes.";
RL J. Gen. Microbiol. 138:1051-1065(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Dual-function protein that regulates the transcription of
CC class 2 flagellar operons and that also acts as an export chaperone for
CC the filament-capping protein FliD. As a transcriptional regulator, acts
CC as an anti-FlhDC factor; it directly binds FlhC, thus inhibiting the
CC binding of the FlhC/FlhD complex to class 2 promoters, resulting in
CC decreased expression of class 2 flagellar operons. As a chaperone,
CC effects FliD transition to the membrane by preventing its premature
CC polymerization, and by directing it to the export apparatus.
CC {ECO:0000255|HAMAP-Rule:MF_01180}.
CC -!- SUBUNIT: Homodimer. Interacts with FliD and FlhC. {ECO:0000255|HAMAP-
CC Rule:MF_01180}.
CC -!- INTERACTION:
CC P0ABY2; P24216: fliD; NbExp=4; IntAct=EBI-1114780, EBI-1126352;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the FliT family. {ECO:0000255|HAMAP-
CC Rule:MF_01180}.
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DR EMBL; M85240; AAA23792.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74993.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15754.1; -; Genomic_DNA.
DR PIR; C64956; C64956.
DR RefSeq; NP_416436.1; NC_000913.3.
DR RefSeq; WP_001015033.1; NZ_LN832404.1.
DR AlphaFoldDB; P0ABY2; -.
DR SMR; P0ABY2; -.
DR BioGRID; 4260367; 21.
DR BioGRID; 850790; 4.
DR IntAct; P0ABY2; 21.
DR STRING; 511145.b1926; -.
DR PaxDb; P0ABY2; -.
DR PRIDE; P0ABY2; -.
DR EnsemblBacteria; AAC74993; AAC74993; b1926.
DR EnsemblBacteria; BAA15754; BAA15754; BAA15754.
DR GeneID; 946433; -.
DR KEGG; ecj:JW1911; -.
DR KEGG; eco:b1926; -.
DR PATRIC; fig|1411691.4.peg.323; -.
DR EchoBASE; EB1362; -.
DR eggNOG; ENOG5032ZV7; Bacteria.
DR HOGENOM; CLU_155793_1_1_6; -.
DR OMA; DMEITYL; -.
DR PhylomeDB; P0ABY2; -.
DR BioCyc; EcoCyc:EG11389-MON; -.
DR PRO; PR:P0ABY2; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0044781; P:bacterial-type flagellum organization; IEA:UniProtKB-KW.
DR GO; GO:0071973; P:bacterial-type flagellum-dependent cell motility; IMP:EcoCyc.
DR GO; GO:1902209; P:negative regulation of bacterial-type flagellum assembly; IEA:UniProtKB-UniRule.
DR GO; GO:0006457; P:protein folding; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01180; FliT; 1.
DR InterPro; IPR008622; FliT.
DR Pfam; PF05400; FliT; 1.
PE 1: Evidence at protein level;
KW Bacterial flagellum biogenesis; Chaperone; Cytoplasm; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..121
FT /note="Flagellar protein FliT"
FT /id="PRO_0000180974"
FT REGION 1..50
FT /note="Required for homodimerization"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01180"
FT REGION 60..98
FT /note="FliD binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01180"
SQ SEQUENCE 121 AA; 13829 MW; 5FED8A383B8A1CF8 CRC64;
MNHAPHLYFA WQQLVEKSQL MLRLATEEQW DELIASEMAY VNAVQEIAHL TEEVDPSTTM
QEQLRPMLRL ILDNESKVKQ LLQIRMDELA KLVGQSSVQK SVLSAYGDQG GFVLAPQDNL
F
