ID   FLIW_CAMJ8              Reviewed;         129 AA.
AC   A8FMC8;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Flagellar assembly factor FliW {ECO:0000255|HAMAP-Rule:MF_01185};
GN   Name=fliW {ECO:0000255|HAMAP-Rule:MF_01185}; OrderedLocusNames=C8J_1016;
OS   Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC
OS   11828).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Campylobacteraceae; Campylobacter.
OX   NCBI_TaxID=407148;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=81116 / NCTC 11828;
RX   PubMed=17873037; DOI=10.1128/jb.01404-07;
RA   Pearson B.M., Gaskin D.J.H., Segers R.P.A.M., Wells J.M., Nuijten P.J.M.,
RA   van Vliet A.H.M.;
RT   "The complete genome sequence of Campylobacter jejuni strain 81116
RT   (NCTC11828).";
RL   J. Bacteriol. 189:8402-8403(2007).
RN   [2]
RP   FUNCTION, INTERACTION WITH FLAGELLIN, INTERACTION WITH CSRA, SUBUNIT, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=81116 / NCTC 11828;
RX   PubMed=27353476; DOI=10.1111/mmi.13455;
RA   Radomska K.A., Ordonez S.R., Woesten M.M., Wagenaar J.A., van Putten J.P.;
RT   "Feedback control of Campylobacter jejuni flagellin levels through
RT   reciprocal binding of FliW to flagellin and the global regulator CsrA.";
RL   Mol. Microbiol. 102:207-220(2016).
RN   [3]
RP   INTERACTION WITH FLAA, AND SUBUNIT.
RC   STRAIN=81116 / NCTC 11828;
RX   PubMed=28659885; DOI=10.3389/fmicb.2017.01060;
RA   Radomska K.A., Woesten M.M.S.M., Ordonez S.R., Wagenaar J.A.,
RA   van Putten J.P.M.;
RT   "Importance of Campylobacter jejuni FliS and FliW in flagella biogenesis
RT   and flagellin secretion.";
RL   Front. Microbiol. 8:1060-1060(2017).
CC   -!- FUNCTION: Acts as an anti-CsrA protein, binds CsrA and prevents it from
CC       repressing translation of its target genes, one of which is flagellin.
CC       Binds to flagellin and participates in the assembly of the flagellum.
CC       {ECO:0000255|HAMAP-Rule:MF_01185}.
CC   -!- FUNCTION: Overexpression leads to increased levels of FlaA and FlaB,
CC       but levels of FlaC remain stable (PubMed:27353476). Involved in post-
CC       transcriptional regulation of flagellin biosynthesis (PubMed:27353476).
CC       {ECO:0000269|PubMed:27353476}.
CC   -!- SUBUNIT: Interacts with flagellins FlaA and FlaB but not with FlaC;
CC       recognizes glycosylated and non-glycosylated FlaA equally
CC       (PubMed:27353476, PubMed:28659885). Interacts with CsrA
CC       (PubMed:27353476). May form a 3-way complex of flagellin, FliS and FliW
CC       simultaneously in which FliS and FliW do not directly interact (By
CC       similarity). {ECO:0000250|UniProtKB:P96503,
CC       ECO:0000269|PubMed:27353476}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01185}.
CC   -!- DISRUPTION PHENOTYPE: Cells grow faster and are about 55% less motile
CC       in a swarming assay, flagellar filaments are severely shortened
CC       (PubMed:27353476). Decreased levels of flagellins FlaA and FlaB but not
CC       FlaC accumulate; 3-fold more flaA and 4-fold less flaB mRNA accumulate
CC       in mid-log phase (PubMed:27353476). In double csrA-fliW deletions,
CC       flagellin levels are slightly lower than wild-type (PubMed:27353476).
CC       {ECO:0000269|PubMed:27353476}.
CC   -!- SIMILARITY: Belongs to the FliW family. {ECO:0000255|HAMAP-
CC       Rule:MF_01185}.
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DR   EMBL; CP000814; ABV52615.1; -; Genomic_DNA.
DR   RefSeq; WP_002852982.1; NC_009839.1.
DR   AlphaFoldDB; A8FMC8; -.
DR   SMR; A8FMC8; -.
DR   KEGG; cju:C8J_1016; -.
DR   HOGENOM; CLU_112356_2_0_7; -.
DR   OMA; TIHKMHL; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0044780; P:bacterial-type flagellum assembly; IEA:UniProtKB-UniRule.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.290.10; -; 1.
DR   HAMAP; MF_01185; FliW; 1.
DR   InterPro; IPR003775; Flagellar_assembly_factor_FliW.
DR   InterPro; IPR024046; Flagellar_assmbl_FliW_dom_sf.
DR   PANTHER; PTHR39190; PTHR39190; 1.
DR   Pfam; PF02623; FliW; 1.
DR   SUPFAM; SSF141457; SSF141457; 1.
PE   1: Evidence at protein level;
KW   Bacterial flagellum biogenesis; Chaperone; Cytoplasm;
KW   Translation regulation.
FT   CHAIN           1..129
FT                   /note="Flagellar assembly factor FliW"
FT                   /id="PRO_1000073083"
SQ   SEQUENCE   129 AA;  14844 MW;  B16779B934EDEF31 CRC64;
     MTLAVKCPIL GFEETKNMEF STIDEVFVRL KSLDGKDFSF VLINPYLIRP DYEFDIPTYY
     QELLSLTPES NMKIFNIVAI AKSIEESTVN FLAPVVINLD NNTMVQVILD TVNYPDFFQA
     DQIANYIKK