AL14E_MOUSE
ID AL14E_MOUSE Reviewed; 276 AA.
AC Q8BIX3; Q7TPE6; Q9CZS0;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=ARL14 effector protein;
DE AltName: Full=ARF7 effector protein;
GN Name=Arl14ep; Synonyms=Arf7ep;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Embryo, Olfactory bulb, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=ICR; TISSUE=Trophoblast stem cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
CC -!- FUNCTION: Through its interaction with ARL14 and MYO1E, may connect MHC
CC class II-containing cytoplasmic vesicles to the actin network and hence
CC controls the movement of these vesicles along the actin cytoskeleton in
CC dendritic cells. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with ARL14 and MYO1E. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BIX3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BIX3-2; Sequence=VSP_020776, VSP_020777;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK012215; BAB28102.1; -; mRNA.
DR EMBL; AK076781; BAC36478.1; -; mRNA.
DR EMBL; AK134965; BAE22360.1; -; mRNA.
DR EMBL; BC054106; AAH54106.1; -; mRNA.
DR CCDS; CCDS16503.1; -. [Q8BIX3-1]
DR RefSeq; NP_001020273.1; NM_001025102.1. [Q8BIX3-1]
DR RefSeq; NP_776111.1; NM_173750.2. [Q8BIX3-1]
DR RefSeq; XP_006499149.1; XM_006499086.3.
DR AlphaFoldDB; Q8BIX3; -.
DR IntAct; Q8BIX3; 1.
DR STRING; 10090.ENSMUSP00000028536; -.
DR iPTMnet; Q8BIX3; -.
DR PhosphoSitePlus; Q8BIX3; -.
DR EPD; Q8BIX3; -.
DR jPOST; Q8BIX3; -.
DR MaxQB; Q8BIX3; -.
DR PaxDb; Q8BIX3; -.
DR PeptideAtlas; Q8BIX3; -.
DR PRIDE; Q8BIX3; -.
DR ProteomicsDB; 285803; -. [Q8BIX3-1]
DR ProteomicsDB; 285804; -. [Q8BIX3-2]
DR Antibodypedia; 53638; 118 antibodies from 19 providers.
DR DNASU; 212772; -.
DR Ensembl; ENSMUST00000028536; ENSMUSP00000028536; ENSMUSG00000027122. [Q8BIX3-1]
DR Ensembl; ENSMUST00000093883; ENSMUSP00000091409; ENSMUSG00000027122. [Q8BIX3-1]
DR GeneID; 212772; -.
DR KEGG; mmu:212772; -.
DR UCSC; uc008llq.1; mouse. [Q8BIX3-1]
DR UCSC; uc008lls.1; mouse. [Q8BIX3-2]
DR CTD; 120534; -.
DR MGI; MGI:1926020; Arl14ep.
DR VEuPathDB; HostDB:ENSMUSG00000027122; -.
DR eggNOG; KOG4850; Eukaryota.
DR GeneTree; ENSGT00940000156586; -.
DR HOGENOM; CLU_093502_0_0_1; -.
DR InParanoid; Q8BIX3; -.
DR OMA; CMQIING; -.
DR OrthoDB; 1056242at2759; -.
DR PhylomeDB; Q8BIX3; -.
DR TreeFam; TF333216; -.
DR BioGRID-ORCS; 212772; 7 hits in 58 CRISPR screens.
DR ChiTaRS; Arl14ep; mouse.
DR PRO; PR:Q8BIX3; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8BIX3; protein.
DR Bgee; ENSMUSG00000027122; Expressed in embryonic post-anal tail and 259 other tissues.
DR Genevisible; Q8BIX3; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005925; C:focal adhesion; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR InterPro; IPR029264; ARF7EP_C.
DR InterPro; IPR026515; ARL14EP.
DR PANTHER; PTHR46536; PTHR46536; 1.
DR Pfam; PF14949; ARF7EP_C; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Isopeptide bond; Phosphoprotein;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..276
FT /note="ARL14 effector protein"
FT /id="PRO_0000251893"
FT REGION 158..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 163..177
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 182
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N8R7"
FT MOD_RES 266
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355"
FT CROSSLNK 176
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q8N8R7"
FT VAR_SEQ 142..159
FT /note="GRTAKALRSLQFTNPGKQ -> VCIEAIVLSTVWDFRYME (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_020776"
FT VAR_SEQ 160..276
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_020777"
FT CONFLICT 71
FT /note="Q -> R (in Ref. 2; AAH54106)"
FT /evidence="ECO:0000305"
FT CONFLICT 86
FT /note="K -> R (in Ref. 1; BAB28102)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 276 AA; 31011 MW; FA117F0C09B97BAE CRC64;
MDPCSVGVQL RTTHDCHKTF YTRHTGFKTL KELSSNDMLL LQLRTGMTLS GNNTICLHHV
KIYIDRFEDL QKSCCDPFNI HKKLAKKNLH VIDLDDATFL SAKFGRQLVP GWKLCPKCTQ
IINGSVDVDS DDRQRRKPDS DGRTAKALRS LQFTNPGKQT EFAPEGGKRE KRKLTKATSA
ASDRQIIPAK SKVYDSQGLL IFSGMDLCDC LDEDCLGCFY ACPTCGSTKC GAECRCDRKW
LYEQIEIEGG EIIHNKHAGK AYGLLSPCHP YDILQK
