FLK_ECOLI
ID FLK_ECOLI Reviewed; 331 AA.
AC P15286; P77706; Q9JN62;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Flagellar regulator flk;
DE AltName: Full=Fluke;
GN Name=flk; Synonyms=div; OrderedLocusNames=b2321, JW2318;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=2681152; DOI=10.1128/jb.171.11.6084-6092.1989;
RA Schoenlein P.V., Roa B.B., Winkler M.E.;
RT "Divergent transcription of pdxB and homology between the pdxB and serA
RT gene products in Escherichia coli K-12.";
RL J. Bacteriol. 171:6084-6092(1989).
CC -!- FUNCTION: Acts as a regulator of flagellar gene expression by
CC modulating the protein level of the anti sigma factor FlgM upon sensing
CC ring completion or hook elongation. Flk could inhibit FlgM secretion by
CC acting as a braking system for the flagellar-associated type III
CC secretion (T3S) system. Plays a role in hindering to flip the flagellar
CC T3S specificity switch from the rod and hook-type substrates to
CC filament-type substrates prior to hook-basal body (HBB) completion
CC possibly by preventing interaction of FliK with FlhB (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the flk family. {ECO:0000305}.
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DR EMBL; U00096; AAC75381.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16178.1; -; Genomic_DNA.
DR EMBL; U76961; AAB36529.1; -; Genomic_DNA.
DR EMBL; M29962; AAA24307.2; -; Genomic_DNA.
DR PIR; G65004; G65004.
DR RefSeq; NP_416824.1; NC_000913.3.
DR RefSeq; WP_000615834.1; NZ_LN832404.1.
DR AlphaFoldDB; P15286; -.
DR SMR; P15286; -.
DR BioGRID; 4263217; 44.
DR DIP; DIP-9448N; -.
DR IntAct; P15286; 4.
DR STRING; 511145.b2321; -.
DR jPOST; P15286; -.
DR PaxDb; P15286; -.
DR PRIDE; P15286; -.
DR EnsemblBacteria; AAC75381; AAC75381; b2321.
DR EnsemblBacteria; BAA16178; BAA16178; BAA16178.
DR GeneID; 946776; -.
DR KEGG; ecj:JW2318; -.
DR KEGG; eco:b2321; -.
DR PATRIC; fig|1411691.4.peg.4412; -.
DR EchoBASE; EB0225; -.
DR eggNOG; ENOG502Z805; Bacteria.
DR HOGENOM; CLU_063700_0_0_6; -.
DR OMA; FTHLVTW; -.
DR PhylomeDB; P15286; -.
DR BioCyc; EcoCyc:EG10229-MON; -.
DR PRO; PR:P15286; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0010468; P:regulation of gene expression; IEA:InterPro.
DR InterPro; IPR023597; Flagellar_regulator_Flk.
DR PIRSF; PIRSF020588; Flk; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..331
FT /note="Flagellar regulator flk"
FT /id="PRO_0000079923"
FT TOPO_DOM 1..309
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 331
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..35
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 2
FT /note="Missing (in Ref. 4; AAA24307)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 331 AA; 36668 MW; 9201E5BF4B9D27FB CRC64;
MIQPISGPPP GQPPGQGDNL PSGTGNQPLS SQQRTSLESL MTKVTSLTQQ QRAELWAGIR
HDIGLSGDSP LLSRHFPAAE HNLAQRLLAA QKSHSARQLL AQLGEYLRLG NNRQAVTDYI
RHNFGQTPLN QLSPEQLKTI LTLLQEGKMV IPQPQQREAT DRPLLPAEHN ALKQLVTKLA
AATGEPSKQI WQSMLELSGV KDGELIPAKL FNHLVTWLQA RQTLSQQNTP TLESLQMTLK
QPLDASELAA LSAYIQQKYG LSAQSSLSSA QAEDILNQLY QRRVKGIDPR VMQPLLNPFP
PMMDTLQNMA TRPALWILLV AIILMLVWLV R
