ID   FLMA_ECOLI              Reviewed;          52 AA.
AC   P62670; P16077;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Stable plasmid inheritance protein;
DE   AltName: Full=F leading maintenance protein;
GN   Name=flmA {ECO:0000303|PubMed:3049248};
GN   Synonyms=ORF52 {ECO:0000303|PubMed:3049248}, parB,
GN   stm {ECO:0000303|PubMed:3070354}; OrderedLocusNames=ECOK12F066;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=K12 / C600, K12 / DH5, K12 / MG1655 / ATCC 47076, KL414, and KL623;
RC   PLASMID=F;
RX   PubMed=3070354; DOI=10.1007/bf00337735;
RA   Golub E.I., Panzer H.A.;
RT   "The F factor of Escherichia coli carries a locus of stable plasmid
RT   inheritance stm, similar to the parB locus of plasmid RI.";
RL   Mol. Gen. Genet. 214:353-357(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RC   STRAIN=K12 / AB1157; PLASMID=F;
RX   PubMed=3049248; DOI=10.1016/0378-1119(88)90362-9;
RA   Loh S.M., Cram D.S., Skurray R.A.;
RT   "Nucleotide sequence and transcriptional analysis of a third function (Flm)
RT   involved in F-plasmid maintenance.";
RL   Gene 66:259-268(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63; PLASMID=F;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system
CC       (Probable). Part of the plasmid maintenance system, encodes a toxic
CC       protein that collapses the transmembrane potential and arrests
CC       respiration (By similarity). When the adjacent non-translated flmB
CC       (sok) gene is disrupted FlmA no longer functions in plasmid maintenance
CC       (i.e. FlmB probably encodes an antisense antitoxin RNA)
CC       (PubMed:3070354). The flmA and flmB RNAs interact, and presence of flmB
CC       on a separate plasmid from one encoding flmA and flmB allows loss of
CC       the latter plasmid (PubMed:3049248). Translation of FlmA may be coupled
CC       to the upstream flmC gene (PubMed:3049248).
CC       {ECO:0000250|UniProtKB:P0ACG4, ECO:0000305|PubMed:3049248,
CC       ECO:0000305|PubMed:3070354}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P0ACG4}; Single-pass membrane protein
CC       {ECO:0000305}.
CC   -!- INDUCTION: Part of the flmC-flmA operon; the antisense antitoxin RNA
CC       flmB of this system overlaps the ribosome-binding site and first 3
CC       codons of flmC on the opposite strand. {ECO:0000269|PubMed:3049248}.
CC   -!- SIMILARITY: Belongs to the Hok/Gef family. {ECO:0000305}.
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DR   EMBL; AF106329; AAA99216.1; -; Genomic_DNA.
DR   EMBL; X13521; CAA31872.1; -; Genomic_DNA.
DR   EMBL; AP001918; BAA97936.1; -; Genomic_DNA.
DR   PIR; JS0432; BVECMA.
DR   RefSeq; NP_061445.1; NC_002483.1.
DR   RefSeq; WP_001302184.1; NZ_SSUR01000034.1.
DR   RefSeq; YP_001816514.1; NC_010558.1.
DR   RefSeq; YP_009071221.1; NC_025179.1.
DR   AlphaFoldDB; P62670; -.
DR   SMR; P62670; -.
DR   PRIDE; P62670; -.
DR   PhylomeDB; P62670; -.
DR   PRO; PR:P62670; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR000021; Hok/gef_toxin.
DR   InterPro; IPR018084; Hok/gef_toxin_CS.
DR   Pfam; PF01848; HOK_GEF; 1.
DR   PRINTS; PR00281; HOKGEFTOXIC.
DR   PROSITE; PS00556; HOK_GEF; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Membrane; Plasmid;
KW   Toxin-antitoxin system; Transmembrane; Transmembrane helix.
FT   CHAIN           1..52
FT                   /note="Stable plasmid inheritance protein"
FT                   /id="PRO_0000199045"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        44
FT                   /note="A -> AGYREVAA (in Ref. 1; CAA31872)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   52 AA;  6108 MW;  DFF2E2175DA23586 CRC64;
     MKLPRSSLVW CVLIVCLTLL IFTYLTRKSL CEIRYRDGYR EVAAFMAYES GK