FLO8_CANAL
ID FLO8_CANAL Reviewed; 792 AA.
AC Q59QW5; A0A1D8PQE4; Q7Z883;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 2.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Transcriptional regulator of filamentous growth FLO8;
GN Name=FLO8; OrderedLocusNames=CAALFM_C604350CA;
GN ORFNames=CaO19.1093, CaO19.8695;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP FUNCTION, AND INTERACTION WITH EFG1.
RX PubMed=16267276; DOI=10.1091/mbc.e05-06-0502;
RA Cao F., Lane S., Raniga P.P., Lu Y., Zhou Z., Ramon K., Chen J., Liu H.;
RT "The Flo8 transcription factor is essential for hyphal development and
RT virulence in Candida albicans.";
RL Mol. Biol. Cell 17:295-307(2006).
RN [5]
RP FUNCTION.
RX PubMed=17715361; DOI=10.1128/ec.00199-07;
RA Li Y., Su C., Mao X., Cao F., Chen J.;
RT "Roles of Candida albicans Sfl1 in hyphal development.";
RL Eukaryot. Cell 6:2112-2121(2007).
RN [6]
RP INTERACTION WITH EFG1.
RX PubMed=18375615; DOI=10.1128/ec.00033-08;
RA Noffz C.S., Liedschulte V., Lengeler K., Ernst J.F.;
RT "Functional mapping of the Candida albicans Efg1 regulator.";
RL Eukaryot. Cell 7:881-893(2008).
RN [7]
RP FUNCTION.
RX PubMed=19346360; DOI=10.1128/aem.00098-09;
RA Stichternoth C., Ernst J.F.;
RT "Hypoxic adaptation by Efg1 regulates biofilm formation by Candida
RT albicans.";
RL Appl. Environ. Microbiol. 75:3663-3672(2009).
RN [8]
RP INTERACTION WITH MSS1, FUNCTION, AND DNA-BINDING.
RX PubMed=19734367; DOI=10.1128/ec.00190-09;
RA Su C., Li Y., Lu Y., Chen J.;
RT "Mss11, a transcriptional activator, is required for hyphal development in
RT Candida albicans.";
RL Eukaryot. Cell 8:1780-1791(2009).
RN [9]
RP FUNCTION.
RX PubMed=22621896; DOI=10.1091/mbc.e12-02-0094;
RA Du H., Guan G., Xie J., Cottier F., Sun Y., Jia W., Muhlschlegel F.A.,
RA Huang G.;
RT "The transcription factor Flo8 mediates CO2 sensing in the human fungal
RT pathogen Candida albicans.";
RL Mol. Biol. Cell 23:2692-2701(2012).
CC -!- FUNCTION: Transcription factor which mediates CO(2) sensing. Required
CC for CO(2)-induced white-to-opaque switching, as well as for filamentous
CC growth and virulence. Required for both normoxic and hypoxic biofilm
CC formation. Hypoxic biofilm formation is a major cause of perseverance
CC and antifungal resistance during infections.
CC {ECO:0000269|PubMed:16267276, ECO:0000269|PubMed:17715361,
CC ECO:0000269|PubMed:19346360, ECO:0000269|PubMed:19734367,
CC ECO:0000269|PubMed:22621896}.
CC -!- SUBUNIT: Interacts with EFG1 and MSS11. {ECO:0000269|PubMed:16267276,
CC ECO:0000269|PubMed:18375615, ECO:0000269|PubMed:19734367}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the FLO8 family. {ECO:0000305}.
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DR EMBL; CP017628; AOW30353.1; -; Genomic_DNA.
DR RefSeq; XP_712106.2; XM_707013.2.
DR AlphaFoldDB; Q59QW5; -.
DR SMR; Q59QW5; -.
DR BioGRID; 1229385; 3.
DR STRING; 237561.Q59QW5; -.
DR PRIDE; Q59QW5; -.
DR GeneID; 3646292; -.
DR KEGG; cal:CAALFM_C604350CA; -.
DR CGD; CAL0000178880; FLO8.
DR VEuPathDB; FungiDB:C6_04350C_A; -.
DR eggNOG; KOG0266; Eukaryota.
DR HOGENOM; CLU_345808_0_0_1; -.
DR InParanoid; Q59QW5; -.
DR OrthoDB; 1295908at2759; -.
DR PHI-base; PHI:5056; -.
DR PRO; PR:Q59QW5; -.
DR Proteomes; UP000000559; Chromosome 6.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003714; F:transcription corepressor activity; IEA:InterPro.
DR InterPro; IPR044716; LEUNIG-like.
DR InterPro; IPR006594; LisH.
DR PANTHER; PTHR44376; PTHR44376; 1.
DR Pfam; PF08513; LisH; 1.
DR SMART; SM00667; LisH; 1.
DR PROSITE; PS50896; LISH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Virulence.
FT CHAIN 1..792
FT /note="Transcriptional regulator of filamentous growth
FT FLO8"
FT /id="PRO_0000420157"
FT DOMAIN 6..38
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT REGION 100..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 286..400
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 424..676
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 692..724
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 755..777
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 425..462
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 463..478
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 479..507
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 522..583
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 596..610
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 611..634
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 648..676
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 693..724
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 755..770
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 792 AA; 87127 MW; D27184FEA60ED02E CRC64;
MVPNTTKQVL NSLILDFLVK HQFQDTAKAF SKESPNLPSI PPLMDCSQGF LLEWWQVFFD
LFQVRYGDGN SNNNPNNKLY HDYLRVQETQ KHLFSQLPLI QQQQQQQHHF QQQQQQQGQQ
GQPFSQQQQR GIGVASGMQN QQHQFAPQHQ GQPQGPGQTP QPPGSATNAN FPINMPPNSN
PQQQMFPINQ QFAQMPNGQN QPSMEQQQRM AMMMKQQAMA AQRQQIPMNG LDPQQQQQMM
NAVGGGPGNL NLQQQLFLQQ QQQQQQQPKT TFQQQAQNQM NNLRQQAAMV AQQQQQQQQQ
QQQQGQLQGN LASAMGDSSS KNNSPVGARS NQQSTPQQNA APAPSPHPSQ QGQAQAQHNF
QSQQQQQQQQ MTKMAGSQGM KKNGQMSNGT SNNSSGRNNN ALRDYQNQLM LLERQNKERL
EFARNTGNSD SNPLSNGMMF AGQNQYSNSN QNQNQLPPNQ QQPTPATFHP PPPPTTANGP
QGQFNQKPSP ATSNNSPALG NKSSPAMGNK KSKKESNSKK GKKANSNAST TANNKTSGQT
TPNMSQPPSA GTEPKQPQPT EQMRQLQDKQ QRPGSNTPSM GKKDFQPLTP RSEPISGETT
KKKRKSGKLN DNNENSNGNS PKKQAKTNAN SKNLDPIIKE EENGVLSLKK ESSTSLQDQD
LDLNPPLAPT QATAMSNTFN DDPFDVHLLD TQHHHQQNSN NSNHNRGQNL SNGSNNLSVS
GPGMGMNNSV FGDSTHAFDI NFNIDSLDDI WTTTGPGGDI TGTGSGSGGA GGTDDDNFMG
MNWAADPIEN GD
