FLO8_YEAST
ID FLO8_YEAST Reviewed; 799 AA.
AC P40068; D3DM15; P40067; Q05751;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 25-MAY-2022, entry version 158.
DE RecName: Full=Transcriptional activator FLO8;
DE AltName: Full=Protein PDH5;
GN Name=FLO8; Synonyms=PDH5; OrderedLocusNames=YER109C; ORFNames=YER108C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=Sigma 1278B;
RX PubMed=8913742; DOI=10.1093/genetics/144.3.967;
RA Liu H., Styles C.A., Fink G.R.;
RT "Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for
RT filamentous growth.";
RL Genetics 144:967-978(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=Diastaticus / ATCC 60715 / YIY2-11A;
RX PubMed=8757402; DOI=10.1007/bf02174120;
RA Kobayashi O., Suda H., Ohtani T., Sone H.;
RT "Molecular cloning and analysis of the dominant flocculation gene FLO8 from
RT Saccharomyces cerevisiae.";
RL Mol. Gen. Genet. 251:707-715(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
CC -!- FUNCTION: Required for diploid filamentous growth, haploid invasive
CC growth and flocculation. Putative transcriptional activator of FLO1.
CC {ECO:0000269|PubMed:8757402, ECO:0000269|PubMed:8913742}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the FLO8 family. {ECO:0000305}.
CC -!- CAUTION: Could be the product of a pseudogene unlikely to encode a
CC functional protein. Strain S288c has a stop codon in position 142,
CC which disrupts the gene coding for this protein and produces two ORFs.
CC Because of that it is not part of the S.cerevisiae S288c
CC complete/reference proteome set. {ECO:0000305|PubMed:24374639,
CC ECO:0000305|PubMed:8913742}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC03206.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
CC Sequence=AAC03207.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
CC Sequence=BAA12076.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U51431; AAC49522.1; -; Genomic_DNA.
DR EMBL; D83713; BAA12076.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U18916; AAC03207.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U18916; AAC03206.1; ALT_SEQ; Genomic_DNA.
DR PIR; S50611; S50611.
DR PIR; S50612; S50612.
DR AlphaFoldDB; P40068; -.
DR SMR; P40068; -.
DR BioGRID; 36854; 80.
DR DIP; DIP-5470N; -.
DR IntAct; P40068; 2.
DR MINT; P40068; -.
DR STRING; 4932.YER109C; -.
DR PaxDb; P40068; -.
DR PRIDE; P40068; -.
DR SGD; S000000911; FLO8.
DR eggNOG; ENOG502R28W; Eukaryota.
DR HOGENOM; CLU_351656_0_0_1; -.
DR InParanoid; P40068; -.
DR OMA; NEDIDPM; -.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:SGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR GO; GO:1900189; P:positive regulation of cell adhesion involved in single-species biofilm formation; IMP:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:SGD.
DR InterPro; IPR006594; LisH.
DR SMART; SM00667; LisH; 1.
DR PROSITE; PS50896; LISH; 1.
PE 5: Uncertain;
KW Activator; Nucleus; Transcription; Transcription regulation.
FT CHAIN 1..799
FT /note="Transcriptional activator FLO8"
FT /id="PRO_0000087303"
FT DOMAIN 73..105
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 37..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 101..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 255..406
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 431..503
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 568..622
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 644..691
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 705..758
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..66
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 270..351
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 369..406
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 582..610
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 54
FT /note="Q -> QQQ (in strain: Diastaticus / ATCC 60715 /
FT YIY2-11A)"
FT VARIANT 112
FT /note="V -> I (in strain: Diastaticus / ATCC 60715 / YIY2-
FT 11A)"
FT VARIANT 115
FT /note="P -> S (in strain: Diastaticus / ATCC 60715 / YIY2-
FT 11A)"
FT VARIANT 383
FT /note="C -> G (in strain: Diastaticus / ATCC 60715 / YIY2-
FT 11A)"
FT VARIANT 441
FT /note="A -> T (in strain: Diastaticus / ATCC 60715 / YIY2-
FT 11A)"
FT VARIANT 447
FT /note="A -> V (in strain: Diastaticus / ATCC 60715 / YIY2-
FT 11A)"
FT VARIANT 598
FT /note="R -> P (in strain: Diastaticus / ATCC 60715 / YIY2-
FT 11A)"
SQ SEQUENCE 799 AA; 86835 MW; 91AAE10D8E586DAF CRC64;
MSYKVNSSYP DSIPPTEQPY MASQYKQDLQ SNIAMATNSE QQRQQQQQQQ QQQQQWINQP
TAENSDLKEK MNCKNTLNEY IFDFLTKSSL KNTAAAFAQD AHLDRDKGQN PVDGPKSKEN
NGNQNTFSKV VDTPQGFLYE WWQIFWDIFN TSSSRGGSEF AQQYYQLVLQ EQRQEQIYRS
LAVHAARLQH DAERRGEYSN EDIDPMHLAA MMLGNPMAPA VQMRNVNMNP IPIPMVGNPI
VNNFSIPPYN NANPTTGATA VAPTAPPSGD FTNVGPTQNR SQNVTGWPVY NYPMQPTTEN
PVGNPCNNNT TNNTTNNKSP VNQPKSLKTM HSTDKPNNVP TSKSTRSRSA TSKAKGKVKA
GLVAKRRRKN NTATVSAGST NACSPNITTP GSTTSEPAMV GSRVNKTPRS DIATNFRNQA
IIFGEEDIYS NSKSSPSLDG ASPSALASKQ PTKVRKNTKK ASTSAFPVES TNKLGGNSVV
TGKKRSPPNT RVSRRKSTPS VILNADATKD ENNMLRTFSN TIAPNIHSAP PTKTANSLPF
PGINLGSFNK PAVSSPLSSV TESCFDPESG KIAGKNGPKR AVNSKVSASS PLSIATPRSG
DAQKQRSSKV PGNVVIKPPH GFSTTNLNIT LKNSKIITSQ NNTVSQELPN GGNILEAQVG
NDSRSSKGNR NTLSTPEEKK PSSNNQGYDF DALKNSSSLL FPNQAYASNN RTPNENSNVA
DETSASTNSG DNDNTLIQPS SNVGTTLGPQ QTSTNENQNV HSQNLKFGNI GMVEDQGPDY
DLNLLDTNEN DFNFINWEG
