ID   FLOA_PRIM3              Reviewed;         327 AA.
AC   Q9ZEF9; D5DMN5;
DT   18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-AUG-2010, sequence version 2.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Flotillin-like protein FloA {ECO:0000255|HAMAP-Rule:MF_01562};
GN   Name=floA {ECO:0000255|HAMAP-Rule:MF_01562}; OrderedLocusNames=BMD_4543;
OS   Priestia megaterium (strain DSM 319 / IMG 1521) (Bacillus megaterium).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia.
OX   NCBI_TaxID=592022;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=DSM 319 / IMG 1521;
RX   PubMed=12501411; DOI=10.2323/jgam.44.317;
RA   Wittchen K.-D., Strey J., Bueltmann A., Reichenberg S., Meinhardt F.;
RT   "Molecular characterization of the operon comprising the spoIV gene of
RT   Bacillus megaterium DSM319 and generation of a deletion mutant.";
RL   J. Gen. Appl. Microbiol. 44:317-326(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 319 / IMG 1521;
RX   PubMed=21705586; DOI=10.1128/jb.00449-11;
RA   Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K.,
RA   Koenig S.S., Creasy H.H., Rosovitz M.J., Riley D.R., Daugherty S.,
RA   Martin M., Elbourne L.D., Paulsen I., Biedendieck R., Braun C.,
RA   Grayburn S., Dhingra S., Lukyanchuk V., Ball B., Ul-Qamar R., Seibel J.,
RA   Bremer E., Jahn D., Ravel J., Vary P.S.;
RT   "Genome sequences of the biotechnologically important Bacillus megaterium
RT   strains QM B1551 and DSM319.";
RL   J. Bacteriol. 193:4199-4213(2011).
CC   -!- FUNCTION: Found in functional membrane microdomains (FMM) that may be
CC       equivalent to eukaryotic membrane rafts. FMMs are highly dynamic and
CC       increase in number as cells age. Flotillins are thought to be important
CC       factors in membrane fluidity. {ECO:0000255|HAMAP-Rule:MF_01562}.
CC   -!- SUBUNIT: Homooligomerizes. {ECO:0000255|HAMAP-Rule:MF_01562}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01562};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01562}. Membrane
CC       raft {ECO:0000255|HAMAP-Rule:MF_01562}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_01562}.
CC   -!- INDUCTION: Expressed during sporulation stages IV/V, part of an operon
CC       with downstream gene BMD_4542. {ECO:0000269|PubMed:12501411}.
CC   -!- SIMILARITY: Belongs to the flotillin-like FloA family.
CC       {ECO:0000255|HAMAP-Rule:MF_01562}.
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DR   EMBL; AJ224829; CAA12151.1; -; Genomic_DNA.
DR   EMBL; CP001982; ADF41367.1; -; Genomic_DNA.
DR   RefSeq; WP_013085055.1; NC_014103.1.
DR   AlphaFoldDB; Q9ZEF9; -.
DR   SMR; Q9ZEF9; -.
DR   EnsemblBacteria; ADF41367; ADF41367; BMD_4543.
DR   KEGG; bmd:BMD_4543; -.
DR   PATRIC; fig|592022.4.peg.4539; -.
DR   HOGENOM; CLU_836378_0_0_9; -.
DR   OMA; GIMDYYR; -.
DR   Proteomes; UP000002365; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   HAMAP; MF_01562; FloA; 1.
DR   InterPro; IPR001107; Band_7.
DR   InterPro; IPR022853; FloA.
DR   Pfam; PF12127; YdfA_immunity; 1.
DR   SMART; SM00244; PHB; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..327
FT                   /note="Flotillin-like protein FloA"
FT                   /id="PRO_0000232548"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01562"
FT   TRANSMEM        28..48
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01562"
FT   CONFLICT        242
FT                   /note="A -> R (in Ref. 1; CAA12151)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   327 AA;  35077 MW;  F3417DC4981CD969 CRC64;
     MEVGSVLFFV VIGLAIIALA VFFTFVPIML WISALAAGVR ISIFTLVGMR LRRVIPSRVV
     NPLIKASKAG LGITINQLES HYLAGGNVDR VVNALIAAHR ANIELTFERG AAIDLAGRDV
     LEAVQMSVNP KVIETPFIAG VAMDGIEVKA KARITVRANI DRLVGGAGEE TIIARVGEGI
     VSTIGSQTDH KKVLENPDMI SQTVLGKGLD SGTAFEILSI DIADIDIGKN IGAVLQTDQA
     EADKNIAQAK AEERRAMAVA QEQEMRAKVE EMRAKVVEAE AEVPLAMAEA LRSGNIGVMD
     YMNIQNLTAD TDMRDSIGKM SKEDDEK