ID   FLOA_STAAN              Reviewed;         329 AA.
AC   Q7A5C5;
DT   18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Flotillin-like protein FloA {ECO:0000255|HAMAP-Rule:MF_01562};
GN   Name=floA {ECO:0000255|HAMAP-Rule:MF_01562, ECO:0000303|PubMed:20713508};
GN   OrderedLocusNames=SA1402;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=N315;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=N315;
RA   Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.;
RT   "Shotgun proteomic analysis of total and membrane protein extracts of S.
RT   aureus strain N315.";
RL   Submitted (OCT-2007) to UniProtKB.
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX   PubMed=20713508; DOI=10.1101/gad.1945010;
RA   Lopez D., Kolter R.;
RT   "Functional microdomains in bacterial membranes.";
RL   Genes Dev. 24:1893-1902(2010).
CC   -!- FUNCTION: Found in functional membrane microdomains (FMM) that may be
CC       equivalent to eukaryotic membrane rafts. FMMs are highly dynamic and
CC       increase in number as cells age. Flotillins are thought to be important
CC       factors in membrane fluidity. {ECO:0000255|HAMAP-Rule:MF_01562}.
CC   -!- SUBUNIT: Homooligomerizes. {ECO:0000255|HAMAP-Rule:MF_01562}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01562,
CC       ECO:0000269|PubMed:20713508}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_01562}. Membrane raft {ECO:0000255|HAMAP-
CC       Rule:MF_01562, ECO:0000269|PubMed:20713508}; Multi-pass membrane
CC       protein {ECO:0000255|HAMAP-Rule:MF_01562}. Note=Present in detergent-
CC       resistant membrane (DRM) fractions that may be equivalent to eukaryotic
CC       membrane rafts, with 1 focus per cell; lost when cells are treated with
CC       squalene synthase inhibitor zaragozic acid. These rafts include
CC       proteins involved in signaling, molecule trafficking and protein
CC       secretion. {ECO:0000269|PubMed:20713508}.
CC   -!- SIMILARITY: Belongs to the flotillin-like FloA family.
CC       {ECO:0000255|HAMAP-Rule:MF_01562}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BA000018; BAB42665.1; -; Genomic_DNA.
DR   PIR; D89938; D89938.
DR   RefSeq; WP_000492108.1; NC_002745.2.
DR   AlphaFoldDB; Q7A5C5; -.
DR   SMR; Q7A5C5; -.
DR   EnsemblBacteria; BAB42665; BAB42665; BAB42665.
DR   KEGG; sau:SA1402; -.
DR   HOGENOM; CLU_836378_0_0_9; -.
DR   OMA; GIMDYYR; -.
DR   Proteomes; UP000000751; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   HAMAP; MF_01562; FloA; 1.
DR   InterPro; IPR022853; FloA.
DR   Pfam; PF12127; YdfA_immunity; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..329
FT                   /note="Flotillin-like protein FloA"
FT                   /id="PRO_0000232565"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01562"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01562"
SQ   SEQUENCE   329 AA;  35196 MW;  AA2E49211B31E785 CRC64;
     MFSLSFIVIA VIIIVALLIL FSFVPIGLWI SALAAGVHVG IGTLVGMRLR RVSPRKVIAP
     LIKAHKAGLA LTTNQLESHY LAGGNVDRVV DANIAAQRAD IDLPFERAAA IDLAGRDVLE
     AVQMSVNPKV IETPFIAGVA MNGIEVKAKA RITVRANIAR LVGGAGEETI IARVGEGIVS
     TIGSSKHHTE VLENPDNISK TVLSKGLDSG TAFEILSIDI ADVDISKNIG ADLQTEQALA
     DKNIAQAKAE ERRAMAVATE QEMKARVQEM HAKVVEAESE VPLAMAEALR SGNISVKDYY
     NLKNIEADTG MRNAINKRTD QSDDESPEH