FLOR1_ARATH
ID FLOR1_ARATH Reviewed; 325 AA.
AC Q9LH52; Q93ZC1; Q9C7D9; Q9M7E7;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 142.
DE RecName: Full=Leucine-rich repeat protein FLOR 1 {ECO:0000303|PubMed:11689012};
DE AltName: Full=Protein FLORAL TRANSITION AT THE MERISTEM 4 {ECO:0000303|PubMed:22319055};
GN Name=FLR1 {ECO:0000303|PubMed:11689012};
GN Synonyms=FTM4 {ECO:0000303|PubMed:22319055};
GN OrderedLocusNames=At3g12145 {ECO:0000312|Araport:AT3G12145};
GN ORFNames=F28J15.19 {ECO:0000312|EMBL:AAG51067.1},
GN T21B14.20 {ECO:0000312|EMBL:AEE75158.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 124-139 AND 245-253,
RP INTERACTION WITH AG, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta; TISSUE=Flower;
RX PubMed=11689012; DOI=10.1006/bbrc.2001.5875;
RA Gamboa A., Paez-Valencia J., Acevedo G.F., Vazquez-Moreno L.,
RA Alvarez-Buylla R.E.;
RT "Floral transcription factor AGAMOUS interacts in vitro with a leucine-rich
RT repeat and an acid phosphatase protein complex.";
RL Biochem. Biophys. Res. Commun. 288:1018-1026(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX DOI=10.1016/j.plantsci.2004.03.009;
RA Acevedo F.G., Gamboa A., Paez-Valencia J., Jimenez-Garcia L.F.,
RA Izaguirre-Sierra M., Alvarez-Buylla E.R.;
RT "FLOR1, a putative interaction partner of the floral homeotic protein
RT AGAMOUS, is a plant-specific intracellular LRR.";
RL Plant Sci. 167:225-231(2004).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY,
RP AND INDUCTION BY LONG DAYS.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=22319055; DOI=10.1105/tpc.111.092791;
RA Torti S., Fornara F., Vincent C., Andres F., Nordstrom K., Gobel U.,
RA Knoll D., Schoof H., Coupland G.;
RT "Analysis of the Arabidopsis shoot meristem transcriptome during floral
RT transition identifies distinct regulatory patterns and a leucine-rich
RT repeat protein that promotes flowering.";
RL Plant Cell 24:444-462(2012).
CC -!- FUNCTION: Promotes flowering transition in long days (LD).
CC {ECO:0000269|PubMed:22319055}.
CC -!- SUBUNIT: Interacts with MADS domain transcription factors during flower
CC development. Component of a complex made of FLOR1, VSP1 and AGAMOUS
CC (AG). Binds directly with AG. {ECO:0000269|PubMed:11689012}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|Ref.7}. Nucleus
CC {ECO:0000269|Ref.7}. Cytoplasm, perinuclear region {ECO:0000269|Ref.7}.
CC Cell membrane {ECO:0000269|Ref.7}. Note=In carpels, observed both in
CC cytoplasm and nucleus, as well as in the perinuclear and cell membrane
CC in a vesicle-like pattern. In style cells, present in cytoplasm as well
CC as in nucleus possibly in the perinuclear membrane. In leaf tissue,
CC observed in the plasma membrane and in the perinuclear membrane. In
CC roots and tapetum cells, restricted to the cytoplasm.
CC {ECO:0000269|Ref.7}.
CC -!- TISSUE SPECIFICITY: Confined to flowers and inflorescences (e.g.
CC inflorescence meristems, floral meristems, stamens and carpels).
CC {ECO:0000269|PubMed:11689012, ECO:0000269|PubMed:22319055,
CC ECO:0000269|Ref.7}.
CC -!- DEVELOPMENTAL STAGE: Induced in the early inflorescence meristem
CC (PubMed:22319055). First present when floral induction occurs in
CC inflorescence and floral meristems. Strongly expressed upon flower
CC induction in inflorescence meristems along two lateral strips on the L3
CC of the flank meristem, where flower primordia are initiated. In the L3
CC of the central initiation zone, also present at lower levels.
CC Accumulates at high levels in the L3 of stages 1-4 of flower meristems.
CC In developing flowers, detected from stage 3. By stages 7 and 8,
CC specifically observed in stamen and gynoecium primordia. In stamens,
CC restricted to the anthers, probably to the endothecium, tapetum and the
CC middle layer. In carpels, first uniformly expressed in carpel
CC primordia, but later restricted to the placentae, the funiculus and to
CC the inner and outer integuments (Ref.7, PubMed:22319055).
CC {ECO:0000269|PubMed:22319055, ECO:0000269|Ref.7}.
CC -!- INDUCTION: By shift to long days (LD). {ECO:0000269|PubMed:22319055}.
CC -!- DISRUPTION PHENOTYPE: Delayed flowering. {ECO:0000269|PubMed:22319055}.
CC -!- MISCELLANEOUS: 'Flor' means flower in Spanish.
CC {ECO:0000305|PubMed:11689012}.
CC -!- SIMILARITY: Belongs to the polygalacturonase-inhibiting protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51067.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF136588; AAF65195.1; -; mRNA.
DR EMBL; AP002063; BAB01964.1; -; Genomic_DNA.
DR EMBL; AC069472; AAG51067.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75158.1; -; Genomic_DNA.
DR EMBL; AY057648; AAL15279.1; -; mRNA.
DR EMBL; AY059802; AAL24284.1; -; mRNA.
DR EMBL; BT001172; AAN65059.1; -; mRNA.
DR EMBL; AY085937; AAM63148.1; -; mRNA.
DR RefSeq; NP_974291.4; NM_202562.5.
DR AlphaFoldDB; Q9LH52; -.
DR SMR; Q9LH52; -.
DR STRING; 3702.AT3G12145.1; -.
DR PaxDb; Q9LH52; -.
DR PRIDE; Q9LH52; -.
DR ProteomicsDB; 228971; -.
DR EnsemblPlants; AT3G12145.1; AT3G12145.1; AT3G12145.
DR GeneID; 820391; -.
DR Gramene; AT3G12145.1; AT3G12145.1; AT3G12145.
DR KEGG; ath:AT3G12145; -.
DR Araport; AT3G12145; -.
DR TAIR; locus:2082274; AT3G12145.
DR eggNOG; ENOG502SKQ2; Eukaryota.
DR HOGENOM; CLU_000288_18_22_1; -.
DR InParanoid; Q9LH52; -.
DR OMA; AHNICLC; -.
DR OrthoDB; 1111550at2759; -.
DR PhylomeDB; Q9LH52; -.
DR PRO; PR:Q9LH52; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LH52; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0048574; P:long-day photoperiodism, flowering; IEP:UniProtKB.
DR GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:UniProtKB.
DR GO; GO:0010228; P:vegetative to reproductive phase transition of meristem; IEP:TAIR.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR013210; LRR_N_plant-typ.
DR Pfam; PF00560; LRR_1; 1.
DR Pfam; PF08263; LRRNT_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasm; Direct protein sequencing; Leucine-rich repeat;
KW Membrane; Nucleus; Reference proteome; Repeat.
FT CHAIN 1..325
FT /note="Leucine-rich repeat protein FLOR 1"
FT /id="PRO_5010148311"
FT REPEAT 65..88
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 89..114
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 115..140
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 142..162
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 163..185
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 187..211
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 213..233
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 234..256
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 257..280
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 281..305
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT CONFLICT 23
FT /note="S -> N (in Ref. 1; AAF65195)"
FT /evidence="ECO:0000305"
FT CONFLICT 135..137
FT /note="ISE -> SQ (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 184
FT /note="G -> S (in Ref. 5; AAL15279)"
FT /evidence="ECO:0000305"
FT CONFLICT 252
FT /note="Missing (in Ref. 1; AAF65195)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 325 AA; 35808 MW; 52CF6E6AD87C0B6E CRC64;
MKLFVHLSIF FSILFITLPS SYSCTENDKN ALLQIKKALG NPPLLSSWNP RTDCCTGWTG
VECTNRRVTG LSVTSGEVSG QISYQIGDLV DLRTLDFSYL PHLTGNIPRT ITKLKNLNTL
YLKHTSLSGP IPDYISELKS LTFLDLSFNQ FTGPIPGSLS QMPKLEAIQI NDNKLTGSIP
NSFGSFVGNV PNLYLSNNKL SGKIPESLSK YDFNAVDLSG NGFEGDAFMF FGRNKTTVRV
DLSRNMFNFD LVKVKFARSI VSLDLSQNHI YGKIPPALTK LHLEHFNVSD NHLCGKIPSG
GLLQTFEPSA FAHNICLCGT PLKAC
