FLOT1_ARATH
ID FLOT1_ARATH Reviewed; 470 AA.
AC Q501E6;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Flotillin-like protein 1;
DE Short=AtFLOT1;
DE AltName: Full=Nodulin-like protein 1;
GN Name=FLOT1; OrderedLocusNames=At5g25250; ORFNames=F21J6.108;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP IDENTIFICATION, AND SUBCELLULAR LOCATION.
RX PubMed=15618420; DOI=10.1104/pp.104.053041;
RA Borner G.H., Sherrier D.J., Weimar T., Michaelson L.V., Hawkins N.D.,
RA Macaskill A., Napier J.A., Beale M.H., Lilley K.S., Dupree P.;
RT "Analysis of detergent-resistant membranes in Arabidopsis. Evidence for
RT plasma membrane lipid rafts.";
RL Plant Physiol. 137:104-116(2005).
CC -!- FUNCTION: May act as a scaffolding protein within caveolar membranes,
CC functionally participating in formation of caveolae or caveolae-like
CC vesicles. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}. Membrane, caveola {ECO:0000269|PubMed:15618420}.
CC -!- PTM: May be palmitoylated. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the band 7/mec-2 family. Flotillin subfamily.
CC {ECO:0000305}.
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DR EMBL; AC006259; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED93417.1; -; Genomic_DNA.
DR EMBL; BT022021; AAY25433.1; -; mRNA.
DR EMBL; AK229271; BAF01135.1; -; mRNA.
DR EMBL; BT026368; ABH04475.1; -; mRNA.
DR RefSeq; NP_197907.1; NM_122434.3.
DR AlphaFoldDB; Q501E6; -.
DR SMR; Q501E6; -.
DR BioGRID; 17871; 3.
DR IntAct; Q501E6; 3.
DR STRING; 3702.AT5G25250.1; -.
DR PaxDb; Q501E6; -.
DR PRIDE; Q501E6; -.
DR ProteomicsDB; 230015; -.
DR EnsemblPlants; AT5G25250.1; AT5G25250.1; AT5G25250.
DR GeneID; 832596; -.
DR Gramene; AT5G25250.1; AT5G25250.1; AT5G25250.
DR KEGG; ath:AT5G25250; -.
DR Araport; AT5G25250; -.
DR TAIR; locus:2146965; AT5G25250.
DR eggNOG; KOG2668; Eukaryota.
DR HOGENOM; CLU_030844_1_1_1; -.
DR InParanoid; Q501E6; -.
DR OMA; QPKINVW; -.
DR OrthoDB; 812555at2759; -.
DR PhylomeDB; Q501E6; -.
DR PRO; PR:Q501E6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q501E6; baseline and differential.
DR Genevisible; Q501E6; AT.
DR GO; GO:0005901; C:caveola; IEA:UniProtKB-SubCell.
DR GO; GO:0005768; C:endosome; IDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0044853; C:plasma membrane raft; IDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0071456; P:cellular response to hypoxia; IEP:TAIR.
DR GO; GO:0006897; P:endocytosis; IEP:TAIR.
DR GO; GO:0010324; P:membrane invagination; IMP:TAIR.
DR GO; GO:0072659; P:protein localization to plasma membrane; IBA:GO_Central.
DR Gene3D; 3.30.479.30; -; 1.
DR InterPro; IPR001107; Band_7.
DR InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR InterPro; IPR027705; Flotillin_fam.
DR PANTHER; PTHR13806; PTHR13806; 1.
DR Pfam; PF01145; Band_7; 1.
DR SUPFAM; SSF117892; SSF117892; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Coiled coil; Lipoprotein; Membrane; Palmitate;
KW Reference proteome.
FT CHAIN 1..470
FT /note="Flotillin-like protein 1"
FT /id="PRO_0000395209"
FT COILED 305..354
FT /evidence="ECO:0000255"
FT LIPID 35
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 470 AA; 52310 MW; 80DD6790B718FC3A CRC64;
MFKVARASQY LAITGAGIED IKLSKKSWVF PWQSCTVFDV SPVNYTFKVQ AMSAEKLPFV
LPAVFTIGPR VDDDDALILY ARLISPHDKD SNHVHELVEG VIEGETRVLA ASMTMEEIFK
GTKEFKKEVF DKVQLELNQF GLVIYNANVK QLVDVPGHEY FSYLGQKTQM EAANQARIDV
SEAKMKGEIG AKERTGLTLQ NAAKIDAESK IISMQRQGEG TKEEIKVRTE VKVFENQKEA
DVAKANAELA MKKAAWTKDA QVAEVEATKA VALREAELQT QVEKMNALTR TEKLKAEFLS
KASVEYETKV QEANWELYNK QKQAEAVLYE KQKQAEAQKA QADAAFYSKQ KEAEGLVALA
SAQGTYLRTL LDAVQNDYSC LRDFLMINNG IYQEIAKTNA MAVRDLQPKI SVWNHGGEQG
GGSGNAMKDI AGLYKMLPPV LDTVYEQTGM QPPAWIGTLR GAEPKQVTRS