FLOT1_DROME
ID FLOT1_DROME Reviewed; 426 AA.
AC O61491; Q9V7D7;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Flotillin-1;
GN Name=Flo1 {ECO:0000312|FlyBase:FBgn0024754}; Synonyms=Flo, Flo-1, FLODM-1;
GN ORFNames=CG8200 {ECO:0000312|FlyBase:FBgn0024754};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RX PubMed=9573373; DOI=10.1016/s0378-1119(98)00064-x;
RA Galbiati F., Volonte D., Goltz J.S., Steele Z., Sen J., Jurcsak J.,
RA Stein D., Stevens L., Lisanti M.P.;
RT "Identification, sequence and developmental expression of invertebrate
RT flotillins from Drosophila melanogaster.";
RL Gene 210:229-237(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: May act as a scaffolding protein within caveolar membranes,
CC functionally participating in formation of caveolae or caveolae-like
CC vesicles. {ECO:0000250}.
CC -!- SUBUNIT: Heterooligomeric complex of flotillins 1 and 2 and caveolins 1
CC and 2. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:9573373};
CC Peripheral membrane protein {ECO:0000269|PubMed:9573373}. Membrane,
CC caveola {ECO:0000269|PubMed:9573373}; Peripheral membrane protein
CC {ECO:0000269|PubMed:9573373}. Note=Membrane-associated protein of
CC caveolae.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Short;
CC IsoId=O61491-1; Sequence=Displayed;
CC Name=Long;
CC IsoId=O61491-2; Sequence=VSP_000506;
CC -!- TISSUE SPECIFICITY: Expressed in brain and ventral nerve cord from
CC stage 12-16 of embryogenesis. {ECO:0000269|PubMed:9573373}.
CC -!- SIMILARITY: Belongs to the band 7/mec-2 family. Flotillin subfamily.
CC {ECO:0000305}.
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DR EMBL; AF044734; AAC39012.1; -; mRNA.
DR EMBL; AE013599; AAF58120.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF58121.1; -; Genomic_DNA.
DR EMBL; AY058794; AAL14023.1; -; mRNA.
DR RefSeq; NP_477358.1; NM_058010.4. [O61491-1]
DR RefSeq; NP_725476.1; NM_166102.2. [O61491-2]
DR AlphaFoldDB; O61491; -.
DR SMR; O61491; -.
DR BioGRID; 62460; 10.
DR DIP; DIP-48829N; -.
DR IntAct; O61491; 4.
DR STRING; 7227.FBpp0086485; -.
DR SwissPalm; O61491; -.
DR PaxDb; O61491; -.
DR PRIDE; O61491; -.
DR DNASU; 36726; -.
DR EnsemblMetazoa; FBtr0087351; FBpp0086484; FBgn0024754. [O61491-1]
DR EnsemblMetazoa; FBtr0087352; FBpp0086485; FBgn0024754. [O61491-2]
DR GeneID; 36726; -.
DR KEGG; dme:Dmel_CG8200; -.
DR UCSC; CG8200-RA; d. melanogaster. [O61491-1]
DR CTD; 36726; -.
DR FlyBase; FBgn0024754; Flo1.
DR VEuPathDB; VectorBase:FBgn0024754; -.
DR eggNOG; KOG2668; Eukaryota.
DR GeneTree; ENSGT00560000077232; -.
DR HOGENOM; CLU_038134_1_0_1; -.
DR InParanoid; O61491; -.
DR OMA; ERQYDSE; -.
DR PhylomeDB; O61491; -.
DR Reactome; R-DME-8980692; RHOA GTPase cycle.
DR Reactome; R-DME-9013026; RHOB GTPase cycle.
DR Reactome; R-DME-9013106; RHOC GTPase cycle.
DR SignaLink; O61491; -.
DR BioGRID-ORCS; 36726; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Flo1; fly.
DR GenomeRNAi; 36726; -.
DR PRO; PR:O61491; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0024754; Expressed in oviduct (Drosophila) and 45 other tissues.
DR Genevisible; O61491; DM.
DR GO; GO:0005737; C:cytoplasm; HDA:FlyBase.
DR GO; GO:0031410; C:cytoplasmic vesicle; IBA:GO_Central.
DR GO; GO:0016600; C:flotillin complex; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005198; F:structural molecule activity; NAS:UniProtKB.
DR GO; GO:1901890; P:positive regulation of cell junction assembly; IBA:GO_Central.
DR GO; GO:2000049; P:positive regulation of cell-cell adhesion mediated by cadherin; IBA:GO_Central.
DR GO; GO:0045807; P:positive regulation of endocytosis; IBA:GO_Central.
DR GO; GO:0070528; P:protein kinase C signaling; IBA:GO_Central.
DR GO; GO:0072659; P:protein localization to plasma membrane; IBA:GO_Central.
DR GO; GO:0002090; P:regulation of receptor internalization; IBA:GO_Central.
DR Gene3D; 3.30.479.30; -; 1.
DR InterPro; IPR001107; Band_7.
DR InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR InterPro; IPR031905; Flotillin_C.
DR InterPro; IPR027705; Flotillin_fam.
DR PANTHER; PTHR13806; PTHR13806; 1.
DR Pfam; PF01145; Band_7; 1.
DR Pfam; PF15975; Flot; 1.
DR SMART; SM00244; PHB; 1.
DR SUPFAM; SSF117892; SSF117892; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Developmental protein; Membrane;
KW Reference proteome.
FT CHAIN 1..426
FT /note="Flotillin-1"
FT /id="PRO_0000094054"
FT VAR_SEQ 162
FT /note="G -> GDSKG (in isoform Long)"
FT /evidence="ECO:0000305"
FT /id="VSP_000506"
SQ SEQUENCE 426 AA; 47135 MW; 64C99F9CB4676C0C CRC64;
MTWGFVTCGP NEALVVSGCC YMKPLLVPGG RAFVWPVGQQ VQRISLNTMT LQVESPCVYT
SQGVPISVTG IAQVKVQGQN EDMLLTACEQ FLGKSEAEIN HIALVTLEGH QRAIMGSMTV
EEIYKDRKKF SKQVFEVASS DLANMGITVV SYTIKDLRDE EGYLRSLGMA RTAEVKRDAR
IGEAEARAEA HIKEAIAEEQ RMAARFLNDT DIAKAQRDFE LKKAAYDVEV QTKKAEAEMA
YELQAAKTKQ RIKEEQMQVK VIERTQEIAV QEQEIMRRER ELEATIRRPA EAEKFRMEKL
AEANKQRVVM EAEAEAESIR IRGEAEAFAI AAKAKAEAEQ MAMKAEAYRE YREAAMVEML
LDTLPKVAAE VAAPLSQAKK ITMVSSGTGD IGAAKLTGEV LSIVNKVPEL VKNITGVDIA
RSVHAG
