ID   FLOT1_MEDTR             Reviewed;         478 AA.
AC   D2XNQ8;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   02-MAR-2010, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Flotillin-like protein 1;
GN   Name=FLOT1;
OS   Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3880;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, AND TISSUE SPECIFICITY.
RX   PubMed=20018678; DOI=10.1073/pnas.0910081107;
RA   Haney C.H., Long S.R.;
RT   "Plant flotillins are required for infection by nitrogen-fixing bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:478-483(2010).
CC   -!- FUNCTION: May act as a scaffolding protein within caveolar membranes,
CC       functionally participating in formation of caveolae or caveolae-like
CC       vesicles (By similarity). May be involved in nodule formation.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}. Membrane, caveola {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in all plant organs. Primarily expressed
CC       in vascular tissues. No change in spatial expression in root upon
CC       inoculation. Expression limited to the nodule vascular tissue.
CC       {ECO:0000269|PubMed:20018678}.
CC   -!- INDUCTION: Not induced during nodulation.
CC       {ECO:0000269|PubMed:20018678}.
CC   -!- PTM: May be palmitoylated. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the band 7/mec-2 family. Flotillin subfamily.
CC       {ECO:0000305}.
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DR   EMBL; GU224278; ADA83094.1; -; mRNA.
DR   RefSeq; XP_003603337.2; XM_003603289.2.
DR   AlphaFoldDB; D2XNQ8; -.
DR   SMR; D2XNQ8; -.
DR   STRING; 3880.AES73588; -.
DR   EnsemblPlants; AES73588; AES73588; MTR_3g106480.
DR   GeneID; 11427312; -.
DR   Gramene; AES73588; AES73588; MTR_3g106480.
DR   eggNOG; KOG2668; Eukaryota.
DR   HOGENOM; CLU_030844_1_1_1; -.
DR   OrthoDB; 812555at2759; -.
DR   ExpressionAtlas; D2XNQ8; differential.
DR   GO; GO:0005901; C:caveola; IEA:UniProtKB-SubCell.
DR   GO; GO:0009877; P:nodulation; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.479.30; -; 1.
DR   InterPro; IPR001107; Band_7.
DR   InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR   InterPro; IPR027705; Flotillin_fam.
DR   PANTHER; PTHR13806; PTHR13806; 1.
DR   Pfam; PF01145; Band_7; 1.
DR   SUPFAM; SSF117892; SSF117892; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Coiled coil; Lipoprotein; Membrane; Nodulation; Palmitate.
FT   CHAIN           1..478
FT                   /note="Flotillin-like protein 1"
FT                   /id="PRO_0000395204"
FT   COILED          235..277
FT                   /evidence="ECO:0000255"
FT   LIPID           35
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   478 AA;  53006 MW;  FE386C146E3C011C CRC64;
     MYRVAKASEY LVITGAGIDD VKLEKKAWIF PGQSCTVFDL SPVNYTFEVQ AMSAEKLPFV
     LPAVFTIGPR VDDYESLLKY AKLISPHDKL SNHVNELVQG IIEGETRVLV ASMTMEEVFR
     GTKEFKQEVF DKVQLELNQF GLWIYNANVK QLVDVPGHEY FSYLGQKTQM EAANQARVDV
     AEAKMKGEIG SKLREGQTIQ NAAKIDAETK VIAMQRAGEG EKQGIKVRTE VKVFENQREA
     EVAEANSELA KKKAAWTMAA QVAELEAAKA VALREAELQG EVERMNALTT TEKLKADFLS
     KASVEYDTKV QEANWELYKK QKEAEAILYE KKAEAEAQKA LADSTFYARK QEAEAELYAK
     KKEAEGIMTL GNAQGAYVST LLNALGNNYT AVRDYLMING GMFQEIAKIN AEAVRGLEPK
     ISIWTNGGDN NGGITEGAMG MKEVAGVYKM LPPLFKTVHE QTGMFPPAWM GSLPDKNS