FLOT1_MOUSE
ID FLOT1_MOUSE Reviewed; 428 AA.
AC O08917;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Flotillin-1;
GN Name=Flot1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 133-152; 220-240 AND
RP 302-311, CHARACTERIZATION, AND SUBCELLULAR LOCATION.
RC TISSUE=Adipocyte, and Lung;
RX PubMed=9153235; DOI=10.1074/jbc.272.21.13793;
RA Bickel P.E., Scherer P.E., Schnitzer J.E., Oh P., Lisanti M.P.,
RA Lodish H.F.;
RT "Flotillin and epidermal surface antigen define a new family of caveolae-
RT associated integral membrane proteins.";
RL J. Biol. Chem. 272:13793-13802(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBUNIT.
RX PubMed=10212252; DOI=10.1074/jbc.274.18.12702;
RA Volonte D., Galbiati F., Li S., Nishiyama K., Okamoto T., Lisanti M.P.;
RT "Flotillins/cavatellins are differentially expressed in cells and tissues
RT and form a hetero-oligomeric complex with caveolins in vivo.
RT Characterization and epitope-mapping of a novel flotillin-1 monoclonal
RT antibody probe.";
RL J. Biol. Chem. 274:12702-12709(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Liver, Lung, Pancreas, and
RC Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May act as a scaffolding protein within caveolar membranes,
CC functionally participating in formation of caveolae or caveolae-like
CC vesicles.
CC -!- SUBUNIT: Heterooligomeric complex of flotillin-1 and flotillin-2 and
CC caveolin-1 and caveolin-2. Interacts with ECPAS (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:O75955};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:O75955}. Endosome
CC {ECO:0000250|UniProtKB:O75955}. Membrane, caveola
CC {ECO:0000269|PubMed:9153235}; Peripheral membrane protein
CC {ECO:0000269|PubMed:9153235}. Melanosome
CC {ECO:0000250|UniProtKB:O75955}. Membrane raft
CC {ECO:0000250|UniProtKB:O75955}. Note=Identified by mass spectrometry in
CC melanosome fractions from stage I to stage IV (By similarity).
CC Membrane-associated protein of caveola (PubMed:9153235).
CC {ECO:0000250|UniProtKB:O75955, ECO:0000269|PubMed:9153235}.
CC -!- TISSUE SPECIFICITY: High expression in brain, white adipose tissue,
CC heart muscle, skeletal muscle and lung. Low expression in spleen, liver
CC and testis.
CC -!- MISCELLANEOUS: Expression increases at least 10-fold as 3T3-L1 cells
CC differentiate into adipocytes.
CC -!- SIMILARITY: Belongs to the band 7/mec-2 family. Flotillin subfamily.
CC {ECO:0000305}.
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DR EMBL; U90435; AAB58583.1; -; mRNA.
DR EMBL; AF145044; AAD33945.1; -; mRNA.
DR EMBL; BC004647; AAH04647.1; -; mRNA.
DR CCDS; CCDS28705.1; -.
DR RefSeq; NP_032053.1; NM_008027.2.
DR RefSeq; XP_006523719.1; XM_006523656.3.
DR AlphaFoldDB; O08917; -.
DR SMR; O08917; -.
DR BioGRID; 199704; 72.
DR IntAct; O08917; 65.
DR MINT; O08917; -.
DR STRING; 10090.ENSMUSP00000001569; -.
DR iPTMnet; O08917; -.
DR PhosphoSitePlus; O08917; -.
DR SwissPalm; O08917; -.
DR EPD; O08917; -.
DR jPOST; O08917; -.
DR PaxDb; O08917; -.
DR PeptideAtlas; O08917; -.
DR PRIDE; O08917; -.
DR ProteomicsDB; 267598; -.
DR Antibodypedia; 655; 419 antibodies from 39 providers.
DR DNASU; 14251; -.
DR Ensembl; ENSMUST00000001569; ENSMUSP00000001569; ENSMUSG00000059714.
DR GeneID; 14251; -.
DR KEGG; mmu:14251; -.
DR UCSC; uc008cip.1; mouse.
DR CTD; 10211; -.
DR MGI; MGI:1100500; Flot1.
DR VEuPathDB; HostDB:ENSMUSG00000059714; -.
DR eggNOG; KOG2668; Eukaryota.
DR GeneTree; ENSGT00560000077232; -.
DR HOGENOM; CLU_038134_1_0_1; -.
DR InParanoid; O08917; -.
DR OMA; ERQYDSE; -.
DR OrthoDB; 812555at2759; -.
DR PhylomeDB; O08917; -.
DR TreeFam; TF324879; -.
DR Reactome; R-MMU-5213460; RIPK1-mediated regulated necrosis.
DR Reactome; R-MMU-5675482; Regulation of necroptotic cell death.
DR Reactome; R-MMU-8849932; Synaptic adhesion-like molecules.
DR Reactome; R-MMU-8980692; RHOA GTPase cycle.
DR Reactome; R-MMU-9013026; RHOB GTPase cycle.
DR Reactome; R-MMU-9013106; RHOC GTPase cycle.
DR BioGRID-ORCS; 14251; 5 hits in 78 CRISPR screens.
DR PRO; PR:O08917; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; O08917; protein.
DR Bgee; ENSMUSG00000059714; Expressed in granulocyte and 280 other tissues.
DR ExpressionAtlas; O08917; baseline and differential.
DR Genevisible; O08917; MM.
DR GO; GO:0005912; C:adherens junction; ISO:MGI.
DR GO; GO:0016323; C:basolateral plasma membrane; ISO:MGI.
DR GO; GO:0005901; C:caveola; IDA:MGI.
DR GO; GO:0044291; C:cell-cell contact zone; ISO:MGI.
DR GO; GO:0005911; C:cell-cell junction; ISO:MGI.
DR GO; GO:0034451; C:centriolar satellite; ISO:MGI.
DR GO; GO:0008180; C:COP9 signalosome; ISO:MGI.
DR GO; GO:0030864; C:cortical actin cytoskeleton; ISO:MGI.
DR GO; GO:0031410; C:cytoplasmic vesicle; ISO:MGI.
DR GO; GO:0098691; C:dopaminergic synapse; ISO:MGI.
DR GO; GO:0005769; C:early endosome; ISO:MGI.
DR GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR GO; GO:0009897; C:external side of plasma membrane; IDA:MGI.
DR GO; GO:0016600; C:flotillin complex; IDA:UniProtKB.
DR GO; GO:0098982; C:GABA-ergic synapse; ISO:MGI.
DR GO; GO:0098978; C:glutamatergic synapse; ISO:MGI.
DR GO; GO:0030027; C:lamellipodium; ISO:MGI.
DR GO; GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0045121; C:membrane raft; IDA:MGI.
DR GO; GO:0005815; C:microtubule organizing center; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0044853; C:plasma membrane raft; IDA:UniProtKB.
DR GO; GO:0098793; C:presynapse; ISO:MGI.
DR GO; GO:0048786; C:presynaptic active zone; ISO:MGI.
DR GO; GO:0042383; C:sarcolemma; IDA:MGI.
DR GO; GO:0045202; C:synapse; ISO:MGI.
DR GO; GO:0001931; C:uropod; ISO:MGI.
DR GO; GO:0035255; F:ionotropic glutamate receptor binding; ISO:MGI.
DR GO; GO:0002020; F:protease binding; ISO:MGI.
DR GO; GO:0007411; P:axon guidance; ISO:MGI.
DR GO; GO:0007409; P:axonogenesis; IMP:MGI.
DR GO; GO:0071360; P:cellular response to exogenous dsRNA; ISO:MGI.
DR GO; GO:0033227; P:dsRNA transport; ISO:MGI.
DR GO; GO:0006897; P:endocytosis; ISO:MGI.
DR GO; GO:0022617; P:extracellular matrix disassembly; ISO:MGI.
DR GO; GO:0044854; P:plasma membrane raft assembly; ISO:MGI.
DR GO; GO:0044857; P:plasma membrane raft organization; ISO:MGI.
DR GO; GO:1901890; P:positive regulation of cell junction assembly; IMP:UniProtKB.
DR GO; GO:2000049; P:positive regulation of cell-cell adhesion mediated by cadherin; IMP:UniProtKB.
DR GO; GO:0001819; P:positive regulation of cytokine production; ISO:MGI.
DR GO; GO:0045807; P:positive regulation of endocytosis; IMP:UniProtKB.
DR GO; GO:0034116; P:positive regulation of heterotypic cell-cell adhesion; ISO:MGI.
DR GO; GO:0032728; P:positive regulation of interferon-beta production; ISO:MGI.
DR GO; GO:1901741; P:positive regulation of myoblast fusion; IMP:UniProtKB.
DR GO; GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; ISO:MGI.
DR GO; GO:0032092; P:positive regulation of protein binding; IMP:UniProtKB.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; ISO:MGI.
DR GO; GO:0048643; P:positive regulation of skeletal muscle tissue development; IMP:UniProtKB.
DR GO; GO:0032226; P:positive regulation of synaptic transmission, dopaminergic; IMP:UniProtKB.
DR GO; GO:0034141; P:positive regulation of toll-like receptor 3 signaling pathway; ISO:MGI.
DR GO; GO:0070528; P:protein kinase C signaling; IMP:UniProtKB.
DR GO; GO:0072659; P:protein localization to plasma membrane; IMP:UniProtKB.
DR GO; GO:0050821; P:protein stabilization; ISO:MGI.
DR GO; GO:0051580; P:regulation of neurotransmitter uptake; ISO:MGI.
DR GO; GO:0002090; P:regulation of receptor internalization; IMP:UniProtKB.
DR GO; GO:0035023; P:regulation of Rho protein signal transduction; IMP:MGI.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; ISO:MGI.
DR Gene3D; 3.30.479.30; -; 1.
DR InterPro; IPR001107; Band_7.
DR InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR InterPro; IPR031905; Flotillin_C.
DR InterPro; IPR027705; Flotillin_fam.
DR PANTHER; PTHR13806; PTHR13806; 1.
DR Pfam; PF01145; Band_7; 1.
DR Pfam; PF15975; Flot; 1.
DR SMART; SM00244; PHB; 1.
DR SUPFAM; SSF117892; SSF117892; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Endosome; Membrane;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..428
FT /note="Flotillin-1"
FT /id="PRO_0000094045"
FT MOD_RES 19
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75955"
FT MOD_RES 163
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75955"
FT MOD_RES 385
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75955"
FT MOD_RES 387
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O75955"
SQ SEQUENCE 428 AA; 47513 MW; 4B17C7EA1263F52C CRC64;
MFFTCGPNEA MVVSGFCRSP PVMVAGGRVF VLPCIQQIQR ISLNTLTLNV KSEKVYTRHG
VPISVTGIAQ VKIQGQNKEM LAAACQMFLG KTEAEIAHIA LETLEGHQRA IMAHMTVEEI
YKDRQKFSEQ VFKVASSDLV NMGISVVSYT LKDIHDDQDY LHSLGKARTA QVQKDARIGE
AEAKRDAGIR EAKAKQEKVS AQCLSEIEMA KAQRDYELKK ATYDIEVNTR RAQADLAYQL
QVAKTKQQIE EQRVQVQVVE RAQQVAVQEQ EIARREKELE ARVRKPAEAE RYRLERLAEA
EKAQLIMQAE AEAESVRMRG EAEAFAIGAR ARAEAEQMAK KAEAFQMYQE AAQLDMLLEK
LPQVAEEISG PLTSANKITL VSSGSGTMGA AKVTGEVLDI LSRLPESVER LTGVSISQVN
HNKPLRTA
