FLOT2_DROME
ID FLOT2_DROME Reviewed; 438 AA.
AC O61492; Q0KHS9; Q8SZW0; Q9I7S0; Q9VXZ9;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-JAN-2003, sequence version 3.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Flotillin-2;
GN Name=Flo2 {ECO:0000312|FlyBase:FBgn0264078};
GN Synonyms=Flo-2 {ECO:0000312|FlyBase:FBgn0264078}, FLODm-2;
GN ORFNames=CG32593 {ECO:0000312|FlyBase:FBgn0264078};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 50-438 (ISOFORM B).
RX PubMed=9573373; DOI=10.1016/s0378-1119(98)00064-x;
RA Galbiati F., Volonte D., Goltz J.S., Steele Z., Sen J., Jurcsak J.,
RA Stein D., Stevens L., Lisanti M.P.;
RT "Identification, sequence and developmental expression of invertebrate
RT flotillins from Drosophila melanogaster.";
RL Gene 210:229-237(1998).
CC -!- FUNCTION: May play a role in axon growth and regeneration. May be
CC involved in epidermal cell adhesion and epidermal structure and
CC function.
CC -!- SUBUNIT: Heterooligomeric complex of flotillins 1 and 2. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}. Note=In neuronal cells,
CC associated with GPI-anchored cell-adhesion molecules. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=A; Synonyms=E;
CC IsoId=O61492-1; Sequence=Displayed;
CC Name=B; Synonyms=F;
CC IsoId=O61492-2; Sequence=VSP_000509;
CC Name=C;
CC IsoId=O61492-3; Sequence=VSP_000507;
CC Name=D;
CC IsoId=O61492-4; Sequence=VSP_000508;
CC -!- SIMILARITY: Belongs to the band 7/mec-2 family. Flotillin subfamily.
CC {ECO:0000305}.
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DR EMBL; AE014298; AAF48407.2; -; Genomic_DNA.
DR EMBL; AE014298; AAN09343.1; -; Genomic_DNA.
DR EMBL; AE014298; AAN09346.1; -; Genomic_DNA.
DR EMBL; AE014298; AAN09348.1; -; Genomic_DNA.
DR EMBL; AY069755; AAL39900.1; -; mRNA.
DR EMBL; BT009962; AAQ22431.1; -; mRNA.
DR EMBL; AF044916; AAC39013.1; -; mRNA.
DR RefSeq; NP_001162759.1; NM_001169288.1. [O61492-4]
DR RefSeq; NP_001259555.1; NM_001272626.2. [O61492-1]
DR RefSeq; NP_511157.2; NM_078602.4. [O61492-2]
DR RefSeq; NP_727797.1; NM_167415.3. [O61492-1]
DR RefSeq; NP_727798.1; NM_167416.3. [O61492-1]
DR RefSeq; NP_727799.1; NM_167417.3. [O61492-2]
DR RefSeq; NP_727812.2; NM_167419.2.
DR RefSeq; NP_727814.1; NM_167421.2. [O61492-4]
DR AlphaFoldDB; O61492; -.
DR SMR; O61492; -.
DR BioGRID; 58783; 16.
DR IntAct; O61492; 34.
DR STRING; 7227.FBpp0303248; -.
DR PaxDb; O61492; -.
DR DNASU; 32425; -.
DR EnsemblMetazoa; FBtr0073949; FBpp0073766; FBgn0264078. [O61492-1]
DR EnsemblMetazoa; FBtr0073950; FBpp0073767; FBgn0264078. [O61492-2]
DR EnsemblMetazoa; FBtr0073951; FBpp0073768; FBgn0264078. [O61492-1]
DR EnsemblMetazoa; FBtr0073952; FBpp0073769; FBgn0264078. [O61492-2]
DR EnsemblMetazoa; FBtr0073954; FBpp0073771; FBgn0264078. [O61492-4]
DR EnsemblMetazoa; FBtr0300435; FBpp0289664; FBgn0264078. [O61492-4]
DR EnsemblMetazoa; FBtr0330216; FBpp0303249; FBgn0264078. [O61492-1]
DR GeneID; 32425; -.
DR KEGG; dme:Dmel_CG32593; -.
DR CTD; 32425; -.
DR FlyBase; FBgn0264078; Flo2.
DR VEuPathDB; VectorBase:FBgn0264078; -.
DR eggNOG; KOG2668; Eukaryota.
DR GeneTree; ENSGT00560000077232; -.
DR InParanoid; O61492; -.
DR PhylomeDB; O61492; -.
DR Reactome; R-DME-8980692; RHOA GTPase cycle.
DR Reactome; R-DME-9013026; RHOB GTPase cycle.
DR Reactome; R-DME-9013106; RHOC GTPase cycle.
DR SignaLink; O61492; -.
DR BioGRID-ORCS; 32425; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Flo2; fly.
DR GenomeRNAi; 32425; -.
DR PRO; PR:O61492; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0264078; Expressed in capitellum (Drosophila) and 30 other tissues.
DR ExpressionAtlas; O61492; baseline and differential.
DR Genevisible; O61492; DM.
DR GO; GO:0005737; C:cytoplasm; HDA:FlyBase.
DR GO; GO:0031410; C:cytoplasmic vesicle; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:FlyBase.
DR GO; GO:0016600; C:flotillin complex; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:FlyBase.
DR GO; GO:0005198; F:structural molecule activity; NAS:UniProtKB.
DR GO; GO:0007155; P:cell adhesion; NAS:UniProtKB.
DR GO; GO:0035011; P:melanotic encapsulation of foreign target; IMP:FlyBase.
DR GO; GO:0051491; P:positive regulation of filopodium assembly; IDA:FlyBase.
DR GO; GO:0061357; P:positive regulation of Wnt protein secretion; IMP:FlyBase.
DR GO; GO:0035593; P:positive regulation of Wnt signaling pathway by establishment of Wnt protein localization to extracellular region; IMP:FlyBase.
DR GO; GO:0072659; P:protein localization to plasma membrane; IBA:GO_Central.
DR GO; GO:0045661; P:regulation of myoblast differentiation; IBA:GO_Central.
DR Gene3D; 3.30.479.30; -; 1.
DR InterPro; IPR001107; Band_7.
DR InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR InterPro; IPR027705; Flotillin_fam.
DR PANTHER; PTHR13806; PTHR13806; 1.
DR Pfam; PF01145; Band_7; 1.
DR SMART; SM00244; PHB; 1.
DR SUPFAM; SSF117892; SSF117892; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell adhesion; Membrane; Reference proteome.
FT CHAIN 1..438
FT /note="Flotillin-2"
FT /id="PRO_0000094055"
FT VAR_SEQ 1..157
FT /note="Missing (in isoform D)"
FT /evidence="ECO:0000305"
FT /id="VSP_000508"
FT VAR_SEQ 1..92
FT /note="MGNIHTTGPNEALIVSGGCCGSTKKRTIVGGWAWAWWLVTDVQRLSLNVMTL
FT NPMCENVETSQGVPLTVTGVAQCKIMKSSSYKQTDYHNDE -> MPSHTCTPTRGHSHN
FT ETTHTHTHTQTLTHTHTNTLTHTHKHKHVQMMNVYYFHHQ (in isoform C)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_000507"
FT VAR_SEQ 80..92
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:9573373, ECO:0000303|Ref.4"
FT /id="VSP_000509"
FT CONFLICT 183
FT /note="A -> G (in Ref. 1; AAC39013)"
FT /evidence="ECO:0000305"
FT CONFLICT 249
FT /note="E -> Q (in Ref. 1; AAC39013)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 438 AA; 48158 MW; 8A842EEF25B05C41 CRC64;
MGNIHTTGPN EALIVSGGCC GSTKKRTIVG GWAWAWWLVT DVQRLSLNVM TLNPMCENVE
TSQGVPLTVT GVAQCKIMKS SSYKQTDYHN DEADELLGTA SEQFLGKSVK EIKQTILQTL
EGHLRAILGT LTVEEVYKDR DQFAALVREV AAPDVGRMGI EILSFTIKDV YDDVQYLASL
GKAQTAVVKR DADAGVAEAN RDAGIREAEC EKSAMDVKYS TDTKIEDNTR MYKLQKANFD
QEINTAKAES QLAYELQAAK IRQRIRNEEI QIEVVERRKQ IEIESQEVQR KDRELTGTVK
LPAEAEAFRL QTLAQAKQCQ TIEGARAEAE RIRKIGSAEA HAIELVGKAE AERMRMKAHV
YKQYGDAAIM NIVLESLPKI AAEVAAPLAK TDEIVLIGGN DNITNDVTRL VAQLPPSINA
LTGVDLSKVL SKIPGAKA
