ID   FLOT2_MEDTR             Reviewed;         480 AA.
AC   D2XNQ9;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   02-MAR-2010, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=Flotillin-like protein 2;
GN   Name=FLOT2;
OS   Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3880;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=20018678; DOI=10.1073/pnas.0910081107;
RA   Haney C.H., Long S.R.;
RT   "Plant flotillins are required for infection by nitrogen-fixing bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:478-483(2010).
CC   -!- FUNCTION: May act as a scaffolding protein within caveolar membranes,
CC       functionally participating in formation of caveolae or caveolae-like
CC       vesicles (By similarity). Required for early symbiotic events and
CC       nodules formation. {ECO:0000250, ECO:0000269|PubMed:20018678}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}. Membrane, caveola {ECO:0000250}. Note=In puncta evenly
CC       distributed in the cell membrane of root hair cells and polarly
CC       localized in root epidermal cells. No changes of localization upon
CC       inoculation with bacteria. {ECO:0000269|PubMed:20018678}.
CC   -!- TISSUE SPECIFICITY: Expressed in flowers in green pods. Primarily
CC       expressed in vascular tissues. Upon induction of nodulation, expansion
CC       of expression in the root cortex in the region of elongating root
CC       hairs, which will eventually become colonized by bacteria. Expressed in
CC       the infection zone in nodules. {ECO:0000269|PubMed:20018678}.
CC   -!- INDUCTION: Up-regulated during nodulation.
CC       {ECO:0000269|PubMed:20018678}.
CC   -!- PTM: May be palmitoylated. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the band 7/mec-2 family. Flotillin subfamily.
CC       {ECO:0000305}.
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DR   EMBL; GU224279; ADA83095.1; -; mRNA.
DR   RefSeq; XP_003603332.1; XM_003603284.2.
DR   AlphaFoldDB; D2XNQ9; -.
DR   SMR; D2XNQ9; -.
DR   STRING; 3880.AES73583; -.
DR   EnsemblPlants; AES73583; AES73583; MTR_3g106420.
DR   GeneID; 11426749; -.
DR   Gramene; AES73583; AES73583; MTR_3g106420.
DR   eggNOG; KOG2668; Eukaryota.
DR   HOGENOM; CLU_030844_1_1_1; -.
DR   OMA; TGGGIQD; -.
DR   OrthoDB; 812555at2759; -.
DR   ExpressionAtlas; D2XNQ9; differential.
DR   GO; GO:0016324; C:apical plasma membrane; IDA:UniProtKB.
DR   GO; GO:0005901; C:caveola; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   GO; GO:0009877; P:nodulation; IMP:UniProtKB.
DR   Gene3D; 3.30.479.30; -; 1.
DR   InterPro; IPR001107; Band_7.
DR   InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR   InterPro; IPR027705; Flotillin_fam.
DR   PANTHER; PTHR13806; PTHR13806; 1.
DR   Pfam; PF01145; Band_7; 1.
DR   SUPFAM; SSF117892; SSF117892; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Coiled coil; Lipoprotein; Membrane; Nodulation; Palmitate.
FT   CHAIN           1..480
FT                   /note="Flotillin-like protein 2"
FT                   /id="PRO_0000395205"
FT   COILED          237..257
FT                   /evidence="ECO:0000255"
FT   LIPID           37
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   480 AA;  53180 MW;  44D00988F2B15C9A CRC64;
     MKIYRVAKAS EYLVITGIFI KDIKLKKKAW IFPGQSCTVL DLSPVNYTFE VQAMSAEKLP
     FVLPAVFTIG PRVDDQESLL KYAKLISPHD RHSNHVNELV QGIIEGETRV LAASMTMEEV
     FRGTKQFKQE VFDKVQLELN QFGLLIYNAN VKQLVDVPGH EYFSYLGQKT QMEAKNQARV
     DVSEAKMKGE IGSKLREGQT LQNAAKIDAE TKVIAMQRAG EGEKEGIKVR TEVKVFENQR
     EAEVAQANSE LAKKKAAWTK AAQVAEVEAK KAVALREAEL QGEVERMNAL TTTEKLKADL
     LSKASVQYET KVQEANWELY KKQKETEAIL YEKKAEAEAQ KASADATFYA SKQAAEAELY
     AKKKEAEGIV TLGQAQGAYV STLLNALGND YTAVRDYLMI NGDMFQEIAK INAEAIRGLE
     PKISIWTNGG DNSGGITDGA MGMKEVAGVY KMLPPLFKTV HEQTGMLPPA WMGALSEKSS