ID   FLOT3_MEDTR             Reviewed;         474 AA.
AC   D2XNR0;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   02-MAR-2010, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Flotillin-like protein 3;
GN   Name=FLOT3;
OS   Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3880;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, AND TISSUE SPECIFICITY.
RX   PubMed=20018678; DOI=10.1073/pnas.0910081107;
RA   Haney C.H., Long S.R.;
RT   "Plant flotillins are required for infection by nitrogen-fixing bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:478-483(2010).
CC   -!- FUNCTION: May act as a scaffolding protein within caveolar membranes,
CC       functionally participating in formation of caveolae or caveolae-like
CC       vesicles (By similarity). May be involved in nodule formation.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}. Membrane, caveola {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in all plant organs. Primarily expressed
CC       in vascular tissues. No change in spatial expression in root upon
CC       inoculation. Expression limited to the nodule vascular tissue.
CC       {ECO:0000269|PubMed:20018678}.
CC   -!- INDUCTION: Not induced during nodulation.
CC       {ECO:0000269|PubMed:20018678}.
CC   -!- PTM: May be palmitoylated. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the band 7/mec-2 family. Flotillin subfamily.
CC       {ECO:0000305}.
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DR   EMBL; GU224280; ADA83096.1; -; mRNA.
DR   RefSeq; XP_013461876.1; XM_013606422.1.
DR   AlphaFoldDB; D2XNR0; -.
DR   SMR; D2XNR0; -.
DR   EnsemblPlants; KEH35911; KEH35911; MTR_3g106485.
DR   GeneID; 25490026; -.
DR   Gramene; KEH35911; KEH35911; MTR_3g106485.
DR   HOGENOM; CLU_030844_1_1_1; -.
DR   OrthoDB; 812555at2759; -.
DR   ExpressionAtlas; D2XNR0; differential.
DR   GO; GO:0005901; C:caveola; IEA:UniProtKB-SubCell.
DR   GO; GO:0009877; P:nodulation; IEA:UniProtKB-KW.
DR   GO; GO:0048364; P:root development; IMP:UniProtKB.
DR   Gene3D; 3.30.479.30; -; 1.
DR   InterPro; IPR001107; Band_7.
DR   InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR   InterPro; IPR027705; Flotillin_fam.
DR   PANTHER; PTHR13806; PTHR13806; 1.
DR   Pfam; PF01145; Band_7; 1.
DR   SUPFAM; SSF117892; SSF117892; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Coiled coil; Lipoprotein; Membrane; Nodulation; Palmitate.
FT   CHAIN           1..474
FT                   /note="Flotillin-like protein 3"
FT                   /id="PRO_0000395206"
FT   COILED          235..255
FT                   /evidence="ECO:0000255"
FT   COILED          305..325
FT                   /evidence="ECO:0000255"
FT   LIPID           35
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   474 AA;  52320 MW;  DF56504B953527B5 CRC64;
     MYRVAKASEY LAITGAGIDD IKLQKKAWIF PGQSCTVFDL SPVNYTFEVQ AMSAEKLPFV
     LPAVFTIGPR VDDQESLLKY AKLISPHDRH SNHVNELVQG IIEGETRVLA ASMTMEEVFR
     GTKQFKQEVF DKVQLELNQF GLLIYNANVK QLVDVPGHEY FSYLGQKTQM EAANQAKVDV
     AEAKMKGEIG SKLRVGQTLQ NAAKIDAETK VIAMQRAGES EKQGIKVRTE VKVFENQREA
     EVAEANSELA KKKAAWTKAA QVAEVEAKKA VALREAELQG EVEKMNALTT TEKLKADLLS
     KASVQYETKV QEANWELYKK QKEAEAILFE KKAEAEAQKA LADSTFYARK QEAEAELYAK
     KKEAEGIVTL GNAQGAYVST LLNALGNNYT AVRDYLMING GMFQEIAKIN AEAVRGLEPK
     ISIWTNGGDN SGGEGAMKEV AGVYKMLPPL FKTVHEQTGM LPPAWMGSLS DKSS