FLOWR_DROME
ID FLOWR_DROME Reviewed; 194 AA.
AC Q95T12; Q8IQM8; Q9VUU2;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Calcium channel flower {ECO:0000303|PubMed:19737521};
DE Short=3L5 {ECO:0000303|PubMed:19737521};
GN Name=fwe; Synonyms=flower; ORFNames=CG6151;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAN11767.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAN11767.1}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAL25424.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL25424.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE
RP SPECIFICITY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF GLU-79 AND GLY-128.
RX PubMed=19737521; DOI=10.1016/j.cell.2009.06.033;
RA Yao C.-K., Lin Y.Q., Ly C.V., Ohyama T., Haueter C.M.,
RA Moiseenkova-Bell V.Y., Wensel T.G., Bellen H.J.;
RT "A synaptic vesicle-associated Ca2+ channel promotes endocytosis and
RT couples exocytosis to endocytosis.";
RL Cell 138:947-960(2009).
CC -!- FUNCTION: A calcium channel that regulates synaptic endocytosis and
CC hence couples exo- with endocytosis. Isoform A and isoform B are mainly
CC required in the nervous system and necessary in photoreceptor cells.
CC {ECO:0000269|PubMed:19737521}.
CC -!- SUBUNIT: Homomultimer; Isoform B. {ECO:0000269|PubMed:19737521}.
CC -!- INTERACTION:
CC Q95T12; Q95T12: fwe; NbExp=7; IntAct=EBI-169467, EBI-169467;
CC Q95T12-2; Q95T12-2: fwe; NbExp=5; IntAct=EBI-2497181, EBI-2497181;
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic
CC vesicle membrane {ECO:0000269|PubMed:19737521}; Multi-pass membrane
CC protein {ECO:0000269|PubMed:19737521}. Note=Upon fusion of the synaptic
CC vesicle with the presynaptic membrane, protein is present in the
CC periactive zones, where endocytosis is known to occur. {ECO:0000255,
CC ECO:0000269|PubMed:19737521}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=A {ECO:0000269|PubMed:10731132, ECO:0000269|PubMed:19737521};
CC IsoId=Q95T12-1; Sequence=Displayed;
CC Name=B {ECO:0000269|PubMed:10731132, ECO:0000269|PubMed:19737521};
CC IsoId=Q95T12-2; Sequence=VSP_053166;
CC Name=C {ECO:0000269|PubMed:10731132, ECO:0000269|PubMed:19737521};
CC IsoId=Q95T12-3; Sequence=VSP_053165;
CC -!- TISSUE SPECIFICITY: Isoform B is expressed in the neuropils of
CC embryonic, larval, adult CNS, and R1-R6 terminals. Expression in the
CC central nervous system (CNS) starts at embryonic stage 13.
CC {ECO:0000269|PubMed:19737521}.
CC -!- DISRUPTION PHENOTYPE: Impaired intracellular resting calcium levels and
CC impaired endocytosis in presynaptic terminals, exocytosis is normal.
CC Mutant boutons at the neuromuscular junctions exhibit a significant
CC depletion in the number of synaptic vesicles. There are numerous extra
CC boutons which are often small, clustered, and flowery in nature. Mutant
CC nerve terminals display omega structures and collared pits.
CC {ECO:0000269|PubMed:19737521}.
CC -!- SIMILARITY: Belongs to the calcium channel flower family.
CC {ECO:0000305}.
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DR EMBL; AE014296; AAF49581.1; -; Genomic_DNA.
DR EMBL; AE014296; AAN11767.1; -; Genomic_DNA.
DR EMBL; AE014296; AAN11768.1; -; Genomic_DNA.
DR EMBL; AY060385; AAL25424.1; -; mRNA.
DR RefSeq; NP_648804.1; NM_140547.5. [Q95T12-1]
DR RefSeq; NP_730071.1; NM_168632.4. [Q95T12-3]
DR RefSeq; NP_730072.1; NM_168633.4. [Q95T12-2]
DR AlphaFoldDB; Q95T12; -.
DR BioGRID; 65034; 8.
DR IntAct; Q95T12; 27.
DR STRING; 7227.FBpp0075297; -.
DR TCDB; 1.A.55.1.2; the synaptic vesicle-associated ca(2+) channel, flower (flower) family.
DR SwissPalm; Q95T12; -.
DR PaxDb; Q95T12; -.
DR PRIDE; Q95T12; -.
DR DNASU; 39720; -.
DR EnsemblMetazoa; FBtr0075541; FBpp0075296; FBgn0261722. [Q95T12-2]
DR EnsemblMetazoa; FBtr0075542; FBpp0075297; FBgn0261722. [Q95T12-1]
DR EnsemblMetazoa; FBtr0075543; FBpp0075298; FBgn0261722. [Q95T12-3]
DR GeneID; 39720; -.
DR KEGG; dme:Dmel_CG6151; -.
DR UCSC; CG6151-RA; d. melanogaster. [Q95T12-1]
DR UCSC; CG6151-RB; d. melanogaster.
DR UCSC; CG6151-RC; d. melanogaster.
DR CTD; 39720; -.
DR FlyBase; FBgn0261722; fwe.
DR VEuPathDB; VectorBase:FBgn0261722; -.
DR eggNOG; KOG4085; Eukaryota.
DR GeneTree; ENSGT00390000000529; -.
DR HOGENOM; CLU_108196_0_0_1; -.
DR InParanoid; Q95T12; -.
DR OMA; CEAPCCC; -.
DR PhylomeDB; Q95T12; -.
DR BioGRID-ORCS; 39720; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 39720; -.
DR PRO; PR:Q95T12; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0261722; Expressed in wing disc and 31 other tissues.
DR ExpressionAtlas; Q95T12; baseline and differential.
DR Genevisible; Q95T12; DM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0030285; C:integral component of synaptic vesicle membrane; IDA:UniProtKB.
DR GO; GO:0044214; C:spanning component of plasma membrane; IDA:FlyBase.
DR GO; GO:0005262; F:calcium channel activity; IDA:FlyBase.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0150008; P:bulk synaptic vesicle endocytosis; IMP:FlyBase.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IMP:FlyBase.
DR GO; GO:0006816; P:calcium ion transport; IDA:FlyBase.
DR GO; GO:0035212; P:cell competition in a multicellular organism; IMP:FlyBase.
DR GO; GO:0150007; P:clathrin-dependent synaptic vesicle endocytosis; IMP:FlyBase.
DR GO; GO:0046530; P:photoreceptor cell differentiation; IMP:UniProtKB.
DR GO; GO:0043525; P:positive regulation of neuron apoptotic process; IMP:FlyBase.
DR GO; GO:0099533; P:positive regulation of presynaptic cytosolic calcium concentration; IMP:FlyBase.
DR GO; GO:0042981; P:regulation of apoptotic process; IMP:FlyBase.
DR GO; GO:0048488; P:synaptic vesicle endocytosis; IMP:UniProtKB.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR InterPro; IPR019365; TVP18/Ca-channel_flower.
DR PANTHER; PTHR13314; PTHR13314; 1.
DR Pfam; PF10233; Cg6151-P; 1.
DR SMART; SM01077; Cg6151-P; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Calcium; Calcium channel; Calcium transport;
KW Cytoplasmic vesicle; Endocytosis; Ion channel; Ion transport; Membrane;
KW Reference proteome; Synapse; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..194
FT /note="Calcium channel flower"
FT /id="PRO_0000389234"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT SITE 79
FT /note="Calcium ion selectivity"
FT /evidence="ECO:0000269|PubMed:19737521"
FT VAR_SEQ 147..194
FT /note="KASAEDMRAAAQQTFGGNTPAQTNDRAGIVNNAQPFSFTGAVGTDSNV ->
FT NKHIFLVRNYI (in isoform C)"
FT /evidence="ECO:0000303|PubMed:10731132,
FT ECO:0000303|PubMed:19737521"
FT /id="VSP_053165"
FT VAR_SEQ 150..194
FT /note="AEDMRAAAQQTFGGNTPAQTNDRAGIVNNAQPFSFTGAVGTDSNV -> RED
FT MAAAATSPTQMAGSQAGGQMQMGGDQHITLMEDPDVWRPT (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10731132,
FT ECO:0000303|PubMed:19737521"
FT /id="VSP_053166"
FT MUTAGEN 79
FT /note="E->Q: Heterologous expression in salivary glands
FT shows inhibition of calcium influx."
FT /evidence="ECO:0000269|PubMed:19737521"
FT MUTAGEN 128
FT /note="G->D: In allele DB25; defect in synaptic
FT transmission and homozygous lethal."
FT /evidence="ECO:0000269|PubMed:19737521"
SQ SEQUENCE 194 AA; 20620 MW; 2D2817158AD73A57 CRC64;
MSFAEKITGL LARPNQQDPI GPEQPWYLKY GSRLLGIVAA FFAILFGLWN VFSIITLSVS
CLVAGILQMV AGFVVMLLEA PCCFVCFGQV NEIAEKVESK PLYFRAGLYI AMAIPPIILC
FGLASLFGSG LIFGTGVVYG MMALGKKASA EDMRAAAQQT FGGNTPAQTN DRAGIVNNAQ
PFSFTGAVGT DSNV
