FLP06_CAEEL
ID FLP06_CAEEL Reviewed; 170 AA.
AC Q19165; P81283; Q21257; Q9TZZ5;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 3.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=FMRFamide-like neuropeptides 6;
DE Contains:
DE RecName: Full=QQDSEVEREMM;
DE Contains:
DE RecName: Full=KSAYMRF-amide 1;
DE AltName: Full=AF8 1;
DE AltName: Full=PF3 1;
DE Contains:
DE RecName: Full=KSAYMRF-amide 2;
DE AltName: Full=AF8 2;
DE AltName: Full=PF3 2;
DE Contains:
DE RecName: Full=KSAYMRF-amide 3;
DE AltName: Full=AF8 3;
DE AltName: Full=PF3 3;
DE Contains:
DE RecName: Full=KSAYMRF-amide 4;
DE AltName: Full=AF8 4;
DE AltName: Full=PF3 4;
DE Contains:
DE RecName: Full=KSAYMRF-amide 5;
DE AltName: Full=AF8 5;
DE AltName: Full=PF3 5;
DE Contains:
DE RecName: Full=KSAYMRF-amide 6;
DE AltName: Full=AF8 6;
DE AltName: Full=PF3 6;
DE Flags: Precursor;
GN Name=flp-6 {ECO:0000312|EMBL:CAA94889.2, ECO:0000312|WormBase:F07D3.2};
GN ORFNames=F07D3.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAC08943.1}
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:AAC08943.1};
RX PubMed=9685599; DOI=10.1016/s0169-328x(98)00106-5;
RA Nelson L.S., Kim K., Memmott J.E., Li C.;
RT "FMRFamide-related gene family in the nematode, Caenorhabditis elegans.";
RL Brain Res. Mol. Brain Res. 58:103-111(1998).
RN [2] {ECO:0000312|EMBL:CAA94889.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP PROTEIN SEQUENCE OF 20-30, AND PYROGLUTAMATE FORMATION AT GLN-20.
RC STRAIN=Bristol N2 {ECO:0000269|PubMed:16061202};
RX PubMed=16061202; DOI=10.1016/j.bbrc.2005.07.044;
RA Husson S.J., Clynen E., Baggerman G., De Loof A., Schoofs L.;
RT "Discovering neuropeptides in Caenorhabditis elegans by two dimensional
RT liquid chromatography and mass spectrometry.";
RL Biochem. Biophys. Res. Commun. 335:76-86(2005).
RN [4] {ECO:0000305}
RP PROTEIN SEQUENCE OF 33-39; 54-60; 84-90; 107-113; 139-145 AND 162-168, MASS
RP SPECTROMETRY, AND AMIDATION AT PHE-39; PHE-60; PHE-90; PHE-113; PHE-145 AND
RP PHE-168.
RX PubMed=9675153; DOI=10.1006/bbrc.1998.8982;
RA Marks N.J., Maule A.G., Geary T.G., Thompson D.P., Li C., Halton D.W.,
RA Shaw C.;
RT "KSAYMRFamide (PF3/AF8) is present in the free-living nematode,
RT Caenorhabditis elegans.";
RL Biochem. Biophys. Res. Commun. 248:422-425(1998).
RN [5]
RP PROTEIN SEQUENCE OF 33-39; 54-60; 84-90; 107-113; 139-145 AND 162-168,
RP AMIDATION AT PHE-39; PHE-60; PHE-90; PHE-113; PHE-145 AND PHE-168, AND MASS
RP SPECTROMETRY.
RX PubMed=28847365; DOI=10.7554/elife.28877;
RA Ohno H., Yoshida M., Sato T., Kato J., Miyazato M., Kojima M., Ida T.,
RA Iino Y.;
RT "Luqin-like RYamide peptides regulate food-evoked responses in C.
RT elegans.";
RL Elife 6:0-0(2017).
RN [6]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=15236235; DOI=10.1002/cne.20189;
RA Kim K., Li C.;
RT "Expression and regulation of an FMRFamide-related neuropeptide gene family
RT in Caenorhabditis elegans.";
RL J. Comp. Neurol. 475:540-550(2004).
RN [7]
RP FUNCTION.
RX PubMed=16187307; DOI=10.1002/neu.20201;
RA Papaioannou S., Marsden D., Franks C.J., Walker R.J., Holden-Dye L.;
RT "Role of a FMRFamide-like family of neuropeptides in the pharyngeal nervous
RT system of Caenorhabditis elegans.";
RL J. Neurobiol. 65:304-319(2005).
CC -!- FUNCTION: FMRFamides and FMRFamide-like peptides are neuropeptides.
CC KSAYMRF-amide has an excitatory effect on dissected pharyngeal myogenic
CC muscle system. {ECO:0000269|PubMed:16187307}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Each flp gene is expressed in a distinct set of
CC neurons. Flp-6 is expressed in the ASE sensory neurons, AFD, ASG, PVT
CC and I1 neurons. {ECO:0000269|PubMed:15236235}.
CC -!- DEVELOPMENTAL STAGE: Expressed from the comma stage of embryogenesis,
CC during all larval stages, and in adults. {ECO:0000269|PubMed:15236235,
CC ECO:0000269|PubMed:9685599}.
CC -!- MASS SPECTROMETRY: [KSAYMRF-amide 1]: Mass=919; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:9675153};
CC -!- MASS SPECTROMETRY: [KSAYMRF-amide 1]: Mass=901.27; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:28847365};
CC -!- SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.
CC {ECO:0000255}.
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DR EMBL; AF042392; AAC08943.1; -; mRNA.
DR EMBL; Z71179; CAA94889.2; -; Genomic_DNA.
DR EMBL; Z70755; CAA94889.2; JOINED; Genomic_DNA.
DR PIR; T20554; T20554.
DR PIR; T42413; T42413.
DR RefSeq; NP_505444.1; NM_073043.3.
DR AlphaFoldDB; Q19165; -.
DR STRING; 6239.F07D3.2; -.
DR PaxDb; Q19165; -.
DR PeptideAtlas; Q19165; -.
DR EnsemblMetazoa; F07D3.2.1; F07D3.2.1; WBGene00001449.
DR GeneID; 179327; -.
DR KEGG; cel:CELE_F07D3.2; -.
DR UCSC; F07D3.2; c. elegans.
DR CTD; 179327; -.
DR WormBase; F07D3.2; CE29252; WBGene00001449; flp-6.
DR eggNOG; ENOG502SF14; Eukaryota.
DR GeneTree; ENSGT00970000197308; -.
DR HOGENOM; CLU_134007_0_0_1; -.
DR InParanoid; Q19165; -.
DR OMA; NPMEMEK; -.
DR OrthoDB; 1525789at2759; -.
DR PhylomeDB; Q19165; -.
DR PRO; PR:Q19165; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00001449; Expressed in larva and 3 other tissues.
DR GO; GO:0005576; C:extracellular region; IC:WormBase.
DR GO; GO:0048018; F:receptor ligand activity; IC:WormBase.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:WormBase.
DR GO; GO:0030718; P:germ-line stem cell population maintenance; IMP:WormBase.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IMP:WormBase.
DR GO; GO:0009408; P:response to heat; IMP:WormBase.
DR InterPro; IPR002544; FMRFamid-related_peptide-like.
DR Pfam; PF01581; FARP; 6.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Neuropeptide; Pyrrolidone carboxylic acid; Reference proteome; Repeat;
KW Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255, ECO:0000303|PubMed:16061202"
FT PEPTIDE 20..30
FT /note="QQDSEVEREMM"
FT /evidence="ECO:0000269|PubMed:16061202"
FT /id="PRO_0000312048"
FT PEPTIDE 33..39
FT /note="KSAYMRF-amide 1"
FT /evidence="ECO:0000269|PubMed:28847365,
FT ECO:0000269|PubMed:9675153"
FT /id="PRO_0000312049"
FT PROPEP 42..51
FT /evidence="ECO:0000255"
FT /id="PRO_0000312050"
FT PEPTIDE 54..60
FT /note="KSAYMRF-amide 2"
FT /evidence="ECO:0000269|PubMed:28847365,
FT ECO:0000269|PubMed:9675153"
FT /id="PRO_0000312051"
FT PROPEP 63..81
FT /evidence="ECO:0000255"
FT /id="PRO_0000312052"
FT PEPTIDE 84..90
FT /note="KSAYMRF-amide 3"
FT /evidence="ECO:0000269|PubMed:28847365,
FT ECO:0000269|PubMed:9675153"
FT /id="PRO_0000312053"
FT PROPEP 93..104
FT /evidence="ECO:0000255"
FT /id="PRO_0000312054"
FT PEPTIDE 107..113
FT /note="KSAYMRF-amide 4"
FT /evidence="ECO:0000269|PubMed:28847365,
FT ECO:0000269|PubMed:9675153"
FT /id="PRO_0000312055"
FT PROPEP 116..136
FT /evidence="ECO:0000255"
FT /id="PRO_0000312056"
FT PEPTIDE 139..145
FT /note="KSAYMRF-amide 5"
FT /evidence="ECO:0000269|PubMed:28847365,
FT ECO:0000269|PubMed:9675153"
FT /id="PRO_0000312057"
FT PROPEP 148..159
FT /evidence="ECO:0000255"
FT /id="PRO_0000312058"
FT PEPTIDE 162..168
FT /note="KSAYMRF-amide 6"
FT /evidence="ECO:0000269|PubMed:28847365,
FT ECO:0000269|PubMed:9675153"
FT /id="PRO_0000312059"
FT REGION 150..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 150..164
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 20
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:16061202"
FT MOD_RES 39
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:28847365,
FT ECO:0000269|PubMed:9675153"
FT MOD_RES 60
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:28847365,
FT ECO:0000269|PubMed:9675153"
FT MOD_RES 90
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:28847365,
FT ECO:0000269|PubMed:9675153"
FT MOD_RES 113
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:28847365,
FT ECO:0000269|PubMed:9675153"
FT MOD_RES 145
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:28847365,
FT ECO:0000269|PubMed:9675153"
FT MOD_RES 168
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:28847365,
FT ECO:0000269|PubMed:9675153"
SQ SEQUENCE 170 AA; 19593 MW; 4FAA09B46E73FF32 CRC64;
MNSRGLILTL GVVIAVAFAQ QDSEVEREMM KRKSAYMRFG RSDGGNPMEM EKRKSAYMRF
GKRSSGGDEQ ELVGGDDIDM EKRKSAYMRF GKRSGPQEDD MPMEKRKSAY MRFGKRSSDM
EVIGNEGVDG DAHDLFKRKS AYMRFGKRSM GEEEDHDMMK RKSAYMRFGR
