FLP2_CAEEL
ID FLP2_CAEEL Reviewed; 106 AA.
AC G5EFN6; G5EDZ1;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=FMRFamide-like neuropeptides 2 {ECO:0000305};
DE Contains:
DE RecName: Full=SPREPIRF-amide {ECO:0000305|PubMed:15809090, ECO:0000305|PubMed:24533288};
DE Contains:
DE RecName: Full=LRGEPIRF-amide {ECO:0000305|PubMed:15809090, ECO:0000305|PubMed:24533288};
DE Flags: Precursor;
GN Name=flp-2 {ECO:0000312|WormBase:W07E11.3a};
GN Synonyms=flp-2a {ECO:0000312|EMBL:AAC08938.1},
GN flp-2b {ECO:0000312|EMBL:AAC08939.1};
GN ORFNames=W07E11.3 {ECO:0000312|WormBase:W07E11.3a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION.
RX PubMed=15809090; DOI=10.1016/j.bbrc.2005.03.071;
RA Mertens I., Meeusen T., Janssen T., Nachman R., Schoofs L.;
RT "Molecular characterization of two G protein-coupled receptor splice
RT variants as FLP2 receptors in Caenorhabditis elegans.";
RL Biochem. Biophys. Res. Commun. 330:967-974(2005).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=24533288; DOI=10.1016/j.ijpddr.2012.10.002;
RA Larsen M.J., Lancheros E.R., Williams T., Lowery D.E., Geary T.G.,
RA Kubiak T.M.;
RT "Functional expression and characterization of the C. elegans G-protein-
RT coupled FLP-2 Receptor (T19F4.1) in mammalian cells and yeast.";
RL Int. J. Parasitol. Drugs Drug Resist. 3:1-7(2013).
RN [4] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=27767096; DOI=10.1038/cr.2016.118;
RA Shao L.W., Niu R., Liu Y.;
RT "Neuropeptide signals cell non-autonomous mitochondrial unfolded protein
RT response.";
RL Cell Res. 26:1182-1196(2016).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=27585848; DOI=10.1534/genetics.116.192898;
RA Chen D., Taylor K.P., Hall Q., Kaplan J.M.;
RT "The Neuropeptides FLP-2 and PDF-1 Act in Concert To Arouse Caenorhabditis
RT elegans Locomotion.";
RL Genetics 204:1151-1159(2016).
CC -!- FUNCTION: FMRFamide-like neuropeptides (PubMed:15809090,
CC PubMed:24533288). Involved in mediating arousal from the sleep-like
CC state called lethargus, which occurs during molting between larval and
CC adult stages, in part by regulating touch sensitivity, and working in
CC concert with neuropeptide pdf-1 (PubMed:27585848). Involved in neural
CC modulation of systemic mitochondrial unfolded protein response
CC (PubMed:27767096). {ECO:0000269|PubMed:15809090,
CC ECO:0000269|PubMed:24533288, ECO:0000269|PubMed:27585848,
CC ECO:0000269|PubMed:27767096}.
CC -!- FUNCTION: [SPREPIRF-amide]: Acts as a ligand to FMRFamide peptide
CC receptor frpr-18 in vitro. {ECO:0000269|PubMed:15809090,
CC ECO:0000269|PubMed:24533288}.
CC -!- FUNCTION: [LRGEPIRF-amide]: Acts as a ligand to FMRFamide peptide
CC receptor frpr-18 in vitro. {ECO:0000269|PubMed:15809090,
CC ECO:0000269|PubMed:24533288}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:W07E11.3a};
CC IsoId=G5EFN6-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:W07E11.3b};
CC IsoId=G5EFN6-2; Sequence=VSP_061253;
CC -!- DISRUPTION PHENOTYPE: Knockouts generated by CRISPR-Cas9-mediated gene
CC editing strongly inhibited the peripheral induction of the
CC mitochondrial unfolded protein response. {ECO:0000269|PubMed:27767096}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF042387; AAC08938.1; -; mRNA.
DR EMBL; AF042388; AAC08939.1; -; mRNA.
DR EMBL; BX284606; CAA90031.1; -; Genomic_DNA.
DR EMBL; BX284606; CAC42354.1; -; Genomic_DNA.
DR PIR; T26263; T26263.
DR PIR; T42409; T42409.
DR RefSeq; NP_001024945.1; NM_001029774.2.
DR AlphaFoldDB; G5EFN6; -.
DR SMR; G5EFN6; -.
DR STRING; 6239.W07E11.3a; -.
DR PaxDb; G5EFN6; -.
DR EnsemblMetazoa; W07E11.3a.1; W07E11.3a.1; WBGene00001445. [G5EFN6-1]
DR EnsemblMetazoa; W07E11.3b.1; W07E11.3b.1; WBGene00001445. [G5EFN6-2]
DR GeneID; 181222; -.
DR CTD; 181222; -.
DR WormBase; W07E11.3a; CE02374; WBGene00001445; flp-2.
DR WormBase; W07E11.3b; CE28258; WBGene00001445; flp-2.
DR eggNOG; ENOG502TI3P; Eukaryota.
DR HOGENOM; CLU_177989_0_0_1; -.
DR InParanoid; G5EFN6; -.
DR OMA; VQPKRIL; -.
DR OrthoDB; 1789029at2759; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00001445; Expressed in larva and 3 other tissues.
DR ExpressionAtlas; G5EFN6; baseline and differential.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IMP:UniProtKB.
DR GO; GO:0040011; P:locomotion; IMP:UniProtKB.
DR GO; GO:0034514; P:mitochondrial unfolded protein response; IMP:UniProtKB.
DR GO; GO:0050714; P:positive regulation of protein secretion; IGI:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IMP:UniProtKB.
DR GO; GO:0030431; P:sleep; IGI:UniProtKB.
PE 3: Inferred from homology;
KW Alternative splicing; Reference proteome; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..106
FT /note="FMRFamide-like neuropeptides 2"
FT /evidence="ECO:0000255"
FT /id="PRO_5015091973"
FT PEPTIDE 75..82
FT /note="LRGEPIRF-amide"
FT /evidence="ECO:0000269|PubMed:15809090"
FT /id="PRO_0000454191"
FT PEPTIDE 86..93
FT /note="SPREPIRF-amide"
FT /evidence="ECO:0000269|PubMed:15809090"
FT /id="PRO_0000454190"
FT REGION 82..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 19..49
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_061253"
SQ SEQUENCE 106 AA; 11897 MW; C8512718002DE1D7 CRC64;
MQVSGILSAL FLVLLAVIVS PFQFVQPKRI LPIPTSRDQL LRGQLAYLKG TTVAQPAVND
NTLGIFEASA MAKRLRGEPI RFGKRSPREP IRFGKRFNPL PDYDFQ
