ID   FLP5_ISOHA              Reviewed;          10 AA.
AC   P0DUV2;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   23-FEB-2022, entry version 2.
DE   RecName: Full=FMRFamide-like peptide Sh5a {ECO:0000303|PubMed:34023397};
DE   AltName: Full=FMRFamide-like peptide Sa-112 {ECO:0000303|PubMed:27096870};
DE            Short=Sa112 {ECO:0000303|PubMed:34023397};
DE   Contains:
DE     RecName: Full=FMRFamide-like peptide Sh5b {ECO:0000303|PubMed:31658776};
OS   Isodontia harmandi (Grass-carrying wasp).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea;
OC   Spheciformes; Sphecidae; Sphecinae; Sphecina; Isodontia.
OX   NCBI_TaxID=2838365;
RN   [1]
RP   PROTEIN SEQUENCE OF 2-10, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR
RP   LOCATION, FUNCTION, SYNTHESIS, AND AMIDATION AT PHE-10.
RC   TISSUE=Venom;
RX   PubMed=31658776; DOI=10.3390/toxins11100585;
RA   Hernandez C., Konno K., Salceda E., Vega R., Zaharenko A.J., Soto E.;
RT   "Sa12b peptide from solitary wasp inhibits ASIC currents in rat dorsal root
RT   ganglion neurons.";
RL   Toxins 11:0-0(2019).
RN   [2]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS
RP   SPECTROMETRY, FUNCTION, AND AMIDATION AT PHE-10.
RA   Nihei K.-I., Kazuma K., Ando K., Konno K.;
RT   "96. Chemical and biological characterization of a novel neuropeptide in
RT   the venom of solitary digger wasp.";
RL   Toxicon 60:144-144(2012).
RN   [3]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND PARTIAL AMIDATION AT PHE-10.
RC   TISSUE=Venom;
RX   PubMed=34023397; DOI=10.1016/j.peptides.2021.170575;
RA   Nihei K.I., Peigneur S., Tytgat J., Lange A.B., Konno K.;
RT   "Isolation and characterization of FMRFamide-like peptides in the venoms of
RT   solitary sphecid wasps.";
RL   Peptides 142:170575-170575(2021).
RN   [4]
RP   REVIEW.
RX   PubMed=27096870; DOI=10.3390/toxins8040114;
RA   Konno K., Kazuma K., Nihei K.;
RT   "Peptide toxins in solitary wasp venoms.";
RL   Toxins 8:114-114(2016).
CC   -!- FUNCTION: [FMRFamide-like peptide Sh5a]: May be directly involved in a
CC       paralyzing effect, by inhibiting muscle contraction, or may also act
CC       centrally to modulate prey behaviors (Probable). Inhibits both the
CC       frequency and amplitude of spontaneous contractions of the locust
CC       oviducts (tested at 50 nM) (Ref.2). {ECO:0000269|Ref.2,
CC       ECO:0000305|PubMed:34023397}.
CC   -!- FUNCTION: [FMRFamide-like peptide Sh5b]: Does not produce consistent
CC       and reproducible effects on ASIC currents (PubMed:31658776). Inhibits
CC       spontaneous oviduct contractions to the same extent as that of
CC       SchistoFLRFamide (Probable). {ECO:0000269|PubMed:31658776,
CC       ECO:0000305|PubMed:34023397}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31658776,
CC       ECO:0000269|PubMed:34023397, ECO:0000269|Ref.2}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:31658776, ECO:0000305|PubMed:34023397,
CC       ECO:0000305|Ref.2}.
CC   -!- PTM: Both the long peptide (Sh5a) and the short peptide (Sh5b) (residue
CC       2-10) are amidated. {ECO:0000269|PubMed:31658776}.
CC   -!- MASS SPECTROMETRY: Mass=1274.6; Method=MALDI; Note=Sh5a, Monoisotopic
CC       mass.; Evidence={ECO:0000269|PubMed:34023397};
CC   -!- MASS SPECTROMETRY: Mass=1145.6; Method=MALDI; Note=Sh5b, Monoisotopic
CC       mass.; Evidence={ECO:0000269|PubMed:34023397};
CC   -!- SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Neurotoxin; Secreted; Toxin.
FT   PEPTIDE         1..10
FT                   /note="FMRFamide-like peptide Sh5a"
FT                   /evidence="ECO:0000269|Ref.2"
FT                   /id="PRO_0000453657"
FT   PEPTIDE         2..10
FT                   /note="FMRFamide-like peptide Sh5b"
FT                   /evidence="ECO:0000269|PubMed:31658776"
FT                   /id="PRO_0000453658"
FT   MOD_RES         10
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:31658776"
SQ   SEQUENCE   10 AA;  1276 MW;  D3D51629D2C1EAB2 CRC64;
     EDVDHVFLRF