ID   FLP_KLULC               Reviewed;         447 AA.
AC   P13783;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Recombinase Flp protein;
GN   Name=A;
OS   Kluyveromyces lactis (Yeast) (Candida sphaerica).
OG   Plasmid pKD1.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=28985;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 56496 / CBS 2105 / CLIB 601 / NRRL Y-8278;
RX   PubMed=3520486; DOI=10.1093/nar/14.11.4471;
RA   Chen X.J., Saliola M., Falcone C., Bianchi M.M., Fukuhara H.;
RT   "Sequence organization of the circular plasmid pKD1 from the yeast
RT   Kluyveromyces drosophilarum.";
RL   Nucleic Acids Res. 14:4471-4481(1986).
CC   -!- FUNCTION: Catalyzes the recombination between the large inverted
CC       repetitions of the plasmid.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; X03961; CAA27591.1; -; Genomic_DNA.
DR   PIR; S28086; S28086.
DR   AlphaFoldDB; P13783; -.
DR   SMR; P13783; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR005626; Recombinase_Flp_C.
DR   InterPro; IPR022647; Recombinase_Flp_N.
DR   Pfam; PF05202; Flp_C; 1.
DR   Pfam; PF03930; Flp_N; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51899; TYR_RECOMBINASE_FLP; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; Plasmid.
FT   CHAIN           1..447
FT                   /note="Recombinase Flp protein"
FT                   /id="PRO_0000197568"
FT   DOMAIN          130..417
FT                   /note="Tyr recombinase Flp-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01247"
FT   REGION          376..401
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        336
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01247"
SQ   SEQUENCE   447 AA;  51110 MW;  89D08AB52718A104 CRC64;
     MSTFAEAAHL TPHQCANEIN EILESDTFNI NAKEIRNKLA SLFSILTMQS LSIRREMKIN
     TYRSYKSAIG KSLSFDKDDK IIKFTVRLRK TESLQKDIES ALPSYKVVVS PFKNQEVSLF
     DRYEETHKYD ASMVGLQFTN ILSKEKDIWK IVSRIACFFD QSCVTTTKRA EYRLLLLGAV
     GNCCRYSDLK NLDPRTFEIY NNSFLGPIVR ATVTETKSRT ERYVNFYPVN GDCDLLISLY
     DYLRVCSPIE KTVSSNRPTN QTHQFLPESL ARTFSRFLTQ HVDEPVFKIW NGPKSHFGRH
     LMATFLSRSE KGKYVSSLGN WAGDREIQSA VARSHYSHGS VTVDDRVFAF ISGFYKEAPL
     GSEIYVLKDP SNKPLSREEL LEEEGNSLGS PPLSPPSSPR LVAQSFSAHP SLQLFEQWHG
     IISDEVLQFI AEYRRKHELR SQRTVVA