ID   FLP_LACFM               Reviewed;         372 AA.
AC   P13770;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Recombinase Flp protein;
OS   Lachancea fermentati (Zygosaccharomyces fermentati).
OG   Plasmid pSM1.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Lachancea.
OX   NCBI_TaxID=4955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NBRC 0021;
RX   PubMed=3680169; DOI=10.1128/jb.169.12.5537-5545.1987;
RA   Utatsu I., Sakamoto S., Imura T., Toh-e A.;
RT   "Yeast plasmids resembling 2 micron DNA: regional similarities and
RT   diversities at the molecular level.";
RL   J. Bacteriol. 169:5537-5545(1987).
CC   -!- FUNCTION: Catalyzes the recombination between the large inverted
CC       repetitions of the plasmid.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; M18275; AAA35279.1; -; Genomic_DNA.
DR   PIR; B34024; B34024.
DR   RefSeq; NP_040495.1; NC_002054.1.
DR   AlphaFoldDB; P13770; -.
DR   SMR; P13770; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR005626; Recombinase_Flp_C.
DR   InterPro; IPR022647; Recombinase_Flp_N.
DR   Pfam; PF05202; Flp_C; 1.
DR   Pfam; PF03930; Flp_N; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51899; TYR_RECOMBINASE_FLP; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; Plasmid.
FT   CHAIN           1..372
FT                   /note="Recombinase Flp protein"
FT                   /id="PRO_0000197571"
FT   DOMAIN          85..367
FT                   /note="Tyr recombinase Flp-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01247"
FT   ACT_SITE        292
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01247"
SQ   SEQUENCE   372 AA;  42876 MW;  6656AC8CB927A469 CRC64;
     MATFSKLSER KRSTFIKYSR EIRQSVQYDR EAQIVKFNYH LKRPHELKDV LDKTFAPIVF
     EVSSTKKVES MVELAAKMDK VEGKGGHNAV AEEITKIVRA DDIWTLLSGV EVTIQKRAFK
     RSLRAELKYV LITSFFNCSR HSDLKNADPT KFELVKNRYL NRVLRVLVCE TKTRKPRYIY
     FFPVNKKTDP LIALHDLFSE AEPVPKSRAS HQKTDQEWQM LRDSLLTNYD RFIATHAKQA
     VFGIKHGPKS HLGRHLMSSY LSHTNHGQWV SPFGNWSAGK DTVESNVARA KYVHIQADIP
     DELFAFLSQY YIQTPSGDFE LIDSSEQPTT FINNLSTQED ISKSYGTWTQ VVGQDVLEYV
     HSYAMGKLGI RK