FLP_MESAU
ID FLP_MESAU Reviewed; 172 AA.
AC Q99MG7;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=Female-specific lacrimal gland protein {ECO:0000303|PubMed:15950295};
DE Short=Female-specific LG protein {ECO:0000303|PubMed:15950295};
DE Flags: Precursor;
GN Name=FLP {ECO:0000303|PubMed:15950295};
OS Mesocricetus auratus (Golden hamster).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Mesocricetus.
OX NCBI_TaxID=10036 {ECO:0000312|EMBL:AAK25813.1};
RN [1] {ECO:0000312|EMBL:AAK25813.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-30, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Lacrimal gland {ECO:0000312|EMBL:AAK25813.1};
RX PubMed=15950295; DOI=10.1016/j.bbaexp.2005.04.008;
RA Srikantan S., Parekh V., De P.K.;
RT "cDNA cloning and regulation of two sex-hormone-repressed hamster tear
RT lipocalins having homology with odorant/pheromone-binding proteins.";
RL Biochim. Biophys. Acta 1729:154-165(2005).
RN [2] {ECO:0000312|EMBL:ABB79786.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Srikantan S., Parekh V., De P.K.;
RT "Cloning and characterization of a gene encoding the female-specific
RT lacrimal protein (FLP) of hamster.";
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=7887957; DOI=10.1006/bbrc.1995.1353;
RA Ranganathan V., De P.K.;
RT "Androgens and estrogens markedly inhibit expression of a 20-kDa major
RT protein in hamster exorbital lacrimal gland.";
RL Biochem. Biophys. Res. Commun. 208:412-417(1995).
RN [4] {ECO:0000305}
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=9634868;
RA De P.K., Ranganathan V.;
RT "Hormonal influences on Syrian hamster lacrimal gland. Marked repression of
RT a major 20 kDa secretory protein by estrogens, androgens, and thyroid
RT hormones.";
RL Adv. Exp. Med. Biol. 438:85-88(1998).
RN [5] {ECO:0000305}
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=10622403; DOI=10.1016/s0960-0760(99)00103-x;
RA Ranganathan V., Jana N.R., De P.K.;
RT "Hormonal effects on hamster lacrimal gland female-specific major 20 kDa
RT secretory protein and its immunological similarity with submandibular gland
RT major male-specific proteins.";
RL J. Steroid Biochem. Mol. Biol. 70:151-158(1999).
RN [6] {ECO:0000305}
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=17316636; DOI=10.1016/j.ygcen.2007.01.003;
RA Srikantan S., Paliwal A., Quintanar-Stephano A., De P.K.;
RT "Estrogen and androgen repression of two female specific lacrimal
RT lipocalins in hamster: Pituitary independent and sex hormone receptor
RT mediated action.";
RL Gen. Comp. Endocrinol. 151:172-179(2007).
RN [7]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=18703064; DOI=10.1016/j.ygcen.2008.07.019;
RA Srikantan S., De P.K.;
RT "Sex differences in expression and differential regulation by androgen and
RT estrogen of two odorant-binding tear lipocalins in lacrimal glands of
RT immature hamsters.";
RL Gen. Comp. Endocrinol. 158:268-276(2008).
RN [8] {ECO:0000305}
RP CRYSTALLIZATION.
RX PubMed=20445246; DOI=10.1107/s1744309110008237;
RA Dubey V.P., Pal B., Srikantan S., Pottabathini S., De P.K.,
RA Sankaranarayanan R.;
RT "Cloning, overexpression, purification, crystallization and preliminary X-
RT ray analysis of a female-specific lipocalin (FLP) expressed in the lacrimal
RT glands of Syrian hamsters.";
RL Acta Crystallogr. F 66:509-512(2010).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15950295,
CC ECO:0000269|PubMed:7887957}. Note=Secreted by the lacrimal gland into
CC tears. {ECO:0000269|PubMed:15950295, ECO:0000269|PubMed:7887957}.
CC -!- TISSUE SPECIFICITY: Expressed in the lacrimal gland from where it is
CC secreted into tears (at protein level). {ECO:0000269|PubMed:10622403,
CC ECO:0000269|PubMed:15950295, ECO:0000269|PubMed:17316636,
CC ECO:0000269|PubMed:18703064, ECO:0000269|PubMed:9634868}.
CC -!- DEVELOPMENTAL STAGE: Expressed at low levels in lacrimal gland of both
CC immature females and males at 10 days of age (at protein level)
CC (PubMed:18703064). At 15 days of age, higher levels are found in males
CC than females (at protein level) (PubMed:18703064). Levels decrease in
CC males after day 20 and there is no expression in males by day 36 (at
CC protein level) (PubMed:18703064). Highly expressed in lactating females
CC when estrogen levels are low (PubMed:15950295, PubMed:9634868,
CC PubMed:10622403). Levels drop by 25 days post-weaning
CC (PubMed:10622403). {ECO:0000269|PubMed:10622403,
CC ECO:0000269|PubMed:15950295, ECO:0000269|PubMed:18703064,
CC ECO:0000269|PubMed:9634868}.
CC -!- INDUCTION: Repressed by estrogens, androgens and thyroxine in adults
CC (at protein level) (PubMed:7887957, PubMed:9634868, PubMed:10622403,
CC PubMed:17316636). Repression by estrogens and androgens does not occur
CC in immature 20-day old animals (PubMed:18703064). Induced by prolonged
CC light deprivation and starvation (at protein level) (PubMed:10622403).
CC {ECO:0000269|PubMed:10622403, ECO:0000269|PubMed:17316636,
CC ECO:0000269|PubMed:18703064, ECO:0000269|PubMed:7887957,
CC ECO:0000269|PubMed:9634868}.
CC -!- SIMILARITY: Belongs to the calycin superfamily. Lipocalin family.
CC {ECO:0000255|RuleBase:RU003695}.
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DR EMBL; AF345648; AAK25813.1; -; mRNA.
DR EMBL; DQ272346; ABB79786.1; -; Genomic_DNA.
DR AlphaFoldDB; Q99MG7; -.
DR SMR; Q99MG7; -.
DR Allergome; 11698; Mes a 1.
DR Proteomes; UP000189706; Unplaced.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0036094; F:small molecule binding; IEA:InterPro.
DR Gene3D; 2.40.128.20; -; 1.
DR InterPro; IPR012674; Calycin.
DR InterPro; IPR002345; Lipocalin.
DR InterPro; IPR022272; Lipocalin_CS.
DR InterPro; IPR000566; Lipocln_cytosolic_FA-bd_dom.
DR InterPro; IPR002448; OBP-like.
DR PANTHER; PTHR11430; PTHR11430; 1.
DR Pfam; PF00061; Lipocalin; 1.
DR PRINTS; PR01173; ODORANTBNDNG.
DR SUPFAM; SSF50814; SSF50814; 1.
DR PROSITE; PS00213; LIPOCALIN; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000269|PubMed:15950295"
FT CHAIN 17..172
FT /note="Female-specific lacrimal gland protein"
FT /evidence="ECO:0000305"
FT /id="PRO_5000059985"
FT DISULFID 60..64
FT /evidence="ECO:0000250|UniProtKB:P08937"
FT DISULFID 79..170
FT /evidence="ECO:0000250|UniProtKB:P08937"
SQ SEQUENCE 172 AA; 19369 MW; F2E29E87B831F629 CRC64;
MVKFLLLALA LGVSCAHYQN LEVSPSEVDG KWYSLYIAAD NKEKVSEGGP LRAYIKNVEC
IDECQTLKIT FYTKVEGVCQ EHTIVGRKGE DGKYITDFSG QNYFHIVEKS DDTMTFHNVN
VDDSGKTNVI LVVGRGESSS IEQKQRFEKT AEEYDIPKEN IEDLVPTDNC DQ
