ID   FLP_ZYGBA               Reviewed;         474 AA.
AC   P13769;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Recombinase Flp protein;
OS   Zygosaccharomyces bailii.
OG   Plasmid pSB2.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Zygosaccharomyces.
OX   NCBI_TaxID=4954;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 56075 / NBRC 1047 / NCTC 6969 / NCYC 128 / CBS 12809;
RX   PubMed=3680169; DOI=10.1128/jb.169.12.5537-5545.1987;
RA   Utatsu I., Sakamoto S., Imura T., Toh-e A.;
RT   "Yeast plasmids resembling 2 micron DNA: regional similarities and
RT   diversities at the molecular level.";
RL   J. Bacteriol. 169:5537-5545(1987).
CC   -!- FUNCTION: Catalyzes the recombination between the large inverted
CC       repetitions of the plasmid.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; M18274; AAA35281.1; -; Genomic_DNA.
DR   PIR; A34024; A34024.
DR   RefSeq; NP_040496.1; NC_002055.1.
DR   AlphaFoldDB; P13769; -.
DR   SMR; P13769; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR005626; Recombinase_Flp_C.
DR   InterPro; IPR022647; Recombinase_Flp_N.
DR   Pfam; PF05202; Flp_C; 1.
DR   Pfam; PF03930; Flp_N; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51899; TYR_RECOMBINASE_FLP; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; Plasmid.
FT   CHAIN           1..474
FT                   /note="Recombinase Flp protein"
FT                   /id="PRO_0000197569"
FT   DOMAIN          135..421
FT                   /note="Tyr recombinase Flp-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01247"
FT   REGION          426..474
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        426..466
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        342
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01247"
SQ   SEQUENCE   474 AA;  54355 MW;  759265A00F25DDD7 CRC64;
     MSEFSELVRI LPLDQVAEIK RILSRGDPIP LQRLASLLTM VILTVNMSKK RKSSPIKLST
     FTKYRRNVAK SLYYDMSSKT VFFEYHLKNT QDLQEGLEQA IAPYNFVVKV HKKPIDWQKQ
     LSSVHERKAG HRSILSNNVG AEISKLAETK DSTWSFIERT MDLIEARTRQ PTTRVAYRFL
     LQLTFMNCCR ANDLKNADPS TFQIIADPHL GRILRAFVPE TKTSIERFIY FFPCKGRCDP
     LLALDSYLLW VGPVPKTQTT DEETQYDYQL LQDTLLISYD RFIAKESKEN IFKIPNGPKA
     HLGRHLMASY LGNNSLKSEA TLYGNWSVER QEGVSKMADS RYMHTVKKSP PSYLFAFLSG
     YYKKSNQGEY VLAETLYNPL DYDKTLPITT NEKLICRRYG KNAKVIPKDA LLYLYTYAQQ
     KRKQLADPNE QNRLFSSESP AHPFLTPQST GSSTPLTWTA PKTLSTGLMT PGEE