ID   FLP_ZYGBI               Reviewed;         568 AA.
AC   P13784;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Recombinase Flp protein;
GN   Name=A;
OS   Zygosaccharomyces bisporus.
OG   Plasmid pSB3.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Zygosaccharomyces.
OX   NCBI_TaxID=4957;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2989791; DOI=10.1093/nar/13.12.4267;
RA   Toh-e A., Utatsu I.;
RT   "Physical and functional structure of a yeast plasmid, pSB3, isolated from
RT   Zygosaccharomyces bisporus.";
RL   Nucleic Acids Res. 13:4267-4283(1985).
CC   -!- FUNCTION: Catalyzes the recombination between the large inverted
CC       repetitions of the plasmid.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; X02608; CAA26455.1; -; Genomic_DNA.
DR   PIR; S28089; S28089.
DR   AlphaFoldDB; P13784; -.
DR   SMR; P13784; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR005626; Recombinase_Flp_C.
DR   InterPro; IPR022647; Recombinase_Flp_N.
DR   Pfam; PF05202; Flp_C; 1.
DR   Pfam; PF03930; Flp_N; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51899; TYR_RECOMBINASE_FLP; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; Plasmid.
FT   CHAIN           1..568
FT                   /note="Recombinase Flp protein"
FT                   /id="PRO_0000197570"
FT   DOMAIN          132..420
FT                   /note="Tyr recombinase Flp-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01247"
FT   REGION          442..517
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          529..568
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        471..495
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        496..517
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        549..568
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        339
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01247"
SQ   SEQUENCE   568 AA;  64671 MW;  7267556A59CA3D39 CRC64;
     MSSYMDLVDD EPATLYHKFV ECLKAGENFC GDKLSGIITM AILKAIKALT EVKKTTFNKY
     KTTIKQGLQY DVGSSTISFV YHLKDCDELS RGLSDAFEPY KFKIKSNKEA TSFKTLFRGP
     SFGSQKNWRK KEVDREVDNL FHSTETDESI FKFILNTLDS IETQTNTDRQ KTVLTFILLM
     TFFNCCRNND LMNVDPSTFK IVKNKFVGYL LQAEVKQTKT RKSRNIFFFP IRENRFDLFL
     ALHDFFRTCQ PTPKSRLSDQ VSEQKWQLFR DSMVIDYNRF FRKFPASPIF AIKHGPKSHL
     GRHLMNSFLH KNELDSWANS LGNWSSSQNQ RESGARLGYT HGGRDLPQPL FGFLAGYCVR
     NEEGHIVGLG LEKDINDLFD GIMDPLNEKE DTEICESYGE WAKIVSKDVL IFLKRYHSKN
     ACRRYQNSTL YARTFLKTES VTLSGSKGSE EPSSPVRIPI LSMGKASPSE GRKLRASEHA
     NDDNEIEKID SDSSQSEEIP IEMSDSEDET TASNISGIYL DMSKANSNVV YSPPSQTGRA
     AGAGRKRGVG GRRTVESKRR RVLAPINR