ID   FLP_ZYGRO               Reviewed;         490 AA.
AC   P13785;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Recombinase Flp protein;
GN   Name=R;
OS   Zygosaccharomyces rouxii (Candida mogii).
OG   Plasmid pSR1.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Zygosaccharomyces.
OX   NCBI_TaxID=4956;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3889347; DOI=10.1016/0022-2836(85)90338-9;
RA   Araki H., Jearnpipatkul A., Tatsumi H., Sakurai T., Ushio T.S.H., Muta T.,
RA   Oshima Y.;
RT   "Molecular and functional organization of yeast plasmid pSR1.";
RL   J. Mol. Biol. 182:191-203(1985).
CC   -!- FUNCTION: Catalyzes the recombination between the large inverted
CC       repetitions of the plasmid.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; X02398; CAA26245.1; -; Genomic_DNA.
DR   PIR; S28355; S28355.
DR   AlphaFoldDB; P13785; -.
DR   SMR; P13785; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR005626; Recombinase_Flp_C.
DR   InterPro; IPR022647; Recombinase_Flp_N.
DR   Pfam; PF05202; Flp_C; 1.
DR   Pfam; PF03930; Flp_N; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51899; TYR_RECOMBINASE_FLP; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; Plasmid.
FT   CHAIN           1..490
FT                   /note="Recombinase Flp protein"
FT                   /id="PRO_0000197572"
FT   DOMAIN          152..437
FT                   /note="Tyr recombinase Flp-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01247"
FT   ACT_SITE        358
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01247"
SQ   SEQUENCE   490 AA;  55755 MW;  D8471C3498F43BDD CRC64;
     MQLTKDTEIS TINRQMSDFS ELSQILPLHQ ISKIKDILEN ENPLPKEKLA SHLTMIILMA
     NLASQKRKDV PVKRSTFLKY QRSISKTLQY DSSTKTVSFE YHLKDPSKLI KGLEDVVSPY
     RFVVGVHEKP DDVMSHLSAV HMRKEAGRKR DLGNKINDEI TKIAETQETI WGFVGKTMDL
     IEARTTRPTT KAAYNLLLQA TFMNCCRADD LKNTDIKTFE VIPDKHLGRM LRAFVPETKT
     GTRFVYFFPC KGRCDPLLAL DSYLQWTDPI PKTRTTDEDA RYDYQLLRNS LLGSYDGFIS
     KQSDESIFKI PNGPKAHLGR HVTASYLSNN EMDKEATLYG NWSAAREEGV SRVAKARYMH
     TIEKSPPSYL FAFLSGFYNI TAERACELVD PNSNPCEQDK NIPMISDIET LMARYGKNAE
     IIPMDVLVFL SSYARFKNNE GKEYKLQARS SRGVPDFPDN GRTALYNALT AAHVKRRKIS
     IVVGRSIDTS