FLR1_YEAST
ID FLR1_YEAST Reviewed; 548 AA.
AC P38124; D6VQ09;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Fluconazole resistance protein 1;
GN Name=FLR1; OrderedLocusNames=YBR008C; ORFNames=YBR0120;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP CHARACTERIZATION.
RX PubMed=9235926; DOI=10.1074/jbc.272.31.19304;
RA Alarco A.-M., Balan I., Talibi D., Mainville N., Raymond M.;
RT "AP1-mediated multidrug resistance in Saccharomyces cerevisiae requires
RT FLR1 encoding a transporter of the major facilitator superfamily.";
RL J. Biol. Chem. 272:19304-19313(1997).
CC -!- FUNCTION: Probable efflux transporter. Confers resistance to the azole
CC derivative fluconazole (FCZ).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; Z35877; CAA84946.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07129.1; -; Genomic_DNA.
DR PIR; S45860; S45860.
DR RefSeq; NP_009562.1; NM_001178356.1.
DR AlphaFoldDB; P38124; -.
DR SMR; P38124; -.
DR BioGRID; 32709; 27.
DR DIP; DIP-4503N; -.
DR STRING; 4932.YBR008C; -.
DR TCDB; 2.A.1.2.17; the major facilitator superfamily (mfs).
DR PaxDb; P38124; -.
DR PRIDE; P38124; -.
DR EnsemblFungi; YBR008C_mRNA; YBR008C; YBR008C.
DR GeneID; 852293; -.
DR KEGG; sce:YBR008C; -.
DR SGD; S000000212; FLR1.
DR VEuPathDB; FungiDB:YBR008C; -.
DR eggNOG; KOG0255; Eukaryota.
DR HOGENOM; CLU_008455_11_1_1; -.
DR InParanoid; P38124; -.
DR OMA; WFESFAI; -.
DR BioCyc; YEAST:G3O-28995-MON; -.
DR PRO; PR:P38124; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38124; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0015244; F:fluconazole transmembrane transporter activity; IMP:SGD.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; IMP:SGD.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004734; Multidrug-R.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00880; 2_A_01_02; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..548
FT /note="Fluconazole resistance protein 1"
FT /id="PRO_0000173435"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 440..460
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 476..496
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 511..531
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 30..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..53
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..77
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 548 AA; 61629 MW; F734B59FCB4ACF75 CRC64;
MVYTSTYRHT IVVDLLEYLG IVSNLETLQS AREDETRKPE NTDKKECKPD YDIECGPNRS
CSESSTDSDS SGSQIEKNDP FRVDWNGPSD PENPQNWPLL KKSLVVFQIM LLTCVTYMGS
SIYTPGQEYI QEEFHVGHVV ATLNLSLYVL GYGLGPIIFS PLSETARYGR LNLYMVTLFF
FMIFQVGCAT VHNIGGLIVM RFISGILCSP SLATGGGTVA DIISPEMVPL VLGMWSAGAV
AAPVLAPLLG AAMVDAKNWR FIFWLLMWLS AATFILLAFF FPETQHHNIL YRRALKLRKE
TGDDRYYTEQ DKLDREVDAR TFLINTLYRP LKMIIKEPAI LAFDLYIAVA YGCFYLFFEA
FPIVFVGIYH FSLVEVGLAY MGFCVGCVLA YGLFGILNMR IIVPRFRNGT FTPEAFLIVA
MCVCWCLPLS LFLFGWTARV HWILPVISEV FFVLAVFNIF QATFAYLATC YPKYVASVFA
GNGFCRASFA CAFPLFGRAM YDNLATKNYP VAWGSSLVGF LTLGLAIIPF ILYKYGPSLR
TRSSYTEE
