FLR2_CANGA
ID FLR2_CANGA Reviewed; 589 AA.
AC Q6FRT5;
DT 28-FEB-2018, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Multidrug transporter FLR2 {ECO:0000303|PubMed:28066366};
DE AltName: Full=Drug:H(+) antiporter FLR2 {ECO:0000303|PubMed:28066366};
DE Short=DHA FLR2 {ECO:0000303|PubMed:28066366};
DE AltName: Full=Flucytosine exporter FLR2 {ECO:0000303|PubMed:28066366};
GN Name=FLR2 {ECO:0000303|PubMed:28066366}; OrderedLocusNames=CAGL0H06039g;
OS Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS Y-65) (Yeast) (Torulopsis glabrata).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC Nakaseomyces/Candida clade.
OX NCBI_TaxID=284593;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
RN [2]
RP DISRUPTION PHENOTYPE, FUNCTION, INDUCTION, AND SUBCELLULAR LOCATION.
RX PubMed=28066366; DOI=10.3389/fmicb.2016.02045;
RA Pais P., Pires C., Costa C., Okamoto M., Chibana H., Teixeira M.C.;
RT "Membrane Proteomics analysis of the Candida glabrata response to 5-
RT flucytosine: unveiling the role and regulation of the drug efflux
RT transporters CgFlr1 and CgFlr2.";
RL Front. Microbiol. 7:2045-2045(2016).
CC -!- FUNCTION: Multidrug transporter that confers resistance to 5-
CC flucytosine (5-FC) and clotrimazole (PubMed:28066366). Further confers
CC azole drug resistance (PubMed:28066366). Plays direct roles in
CC extrusion of 5-flucytosine and clotrimazole (PubMed:28066366).
CC {ECO:0000269|PubMed:28066366}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28066366};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- INDUCTION: Expression is regulated by the transcription factors AP1 and
CC PDR1 (PubMed:28066366). {ECO:0000269|PubMed:28066366}.
CC -!- DISRUPTION PHENOTYPE: Increases the intracellular accumulation of 5-
CC flucytosine and clotrimazole (PubMed:28066366).
CC {ECO:0000269|PubMed:28066366}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; CR380954; CAG59992.1; -; Genomic_DNA.
DR RefSeq; XP_447059.1; XM_447059.1.
DR AlphaFoldDB; Q6FRT5; -.
DR STRING; 5478.XP_447059.1; -.
DR EnsemblFungi; CAG59992; CAG59992; CAGL0H06039g.
DR GeneID; 2888685; -.
DR KEGG; cgr:CAGL0H06039g; -.
DR CGD; CAL0130474; FLR2.
DR VEuPathDB; FungiDB:CAGL0H06039g; -.
DR eggNOG; KOG0255; Eukaryota.
DR HOGENOM; CLU_008455_11_1_1; -.
DR InParanoid; Q6FRT5; -.
DR OMA; RDYMIAI; -.
DR Proteomes; UP000002428; Chromosome H.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:CGD.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; IMP:CGD.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004734; Multidrug-R.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00880; 2_A_01_02; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..589
FT /note="Multidrug transporter FLR2"
FT /id="PRO_0000443412"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 275..295
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 480..500
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 516..536
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 551..571
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 50..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 51..73
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 57
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 136
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 589 AA; 65989 MW; 4CBCC6ED9F5618B3 CRC64;
MYIGAFQDTL FVDMLEYFGW VTVGKEYLDI YRPNGVPVAP NAVAASISGK EEMKQDNQTS
TDSMSTSTQQ ETDASNEDIE RAMDTDNGLD KAMSGGQGVF GTEEDDSSTK DASKPEEADP
FLVEFLGEDD PRKPWNWSFS KKTFVIVQLM VLTCINYMGS SIYTPGQEQI QHEFHVGHVV
GTLNLSMYVL GYAIGPIIFS PLSEVSSIGR MPLYLWTFIL FTILQVACAL VRNIAGLVIL
RFITGILCSP VLATGGASVG DVCFPRYVPR FLGAWAVGAV AAPVMAPILG AAMVVAKDWR
WIFWLMLFMC GATLLSIIFF FPETSHECIL HRRAKRLRKL TGDDRYYTKK EKQEEALPVS
VFIKNTLWRP IKMIALEPII LAFDVYIALC YGAFYLFFEA FPIVFAGIYH FTLVEVGLAF
LGFCVGCVFA YTALIIFQEK VIRKKFLEGK FRPELFLILA MCLGWCLPFS LFFFGWTARI
HWILPIIAEL FFVLSVFNLF QATFSYLAVC YPEYVASVFA GNGLCRGAFA AAFPLFGKAM
YDRLSTKKYP VAWGSTLIGF ITVVLSLIPF VLYKYGPALR ARSRFSPDS
