FLX_ARATH
ID FLX_ARATH Reviewed; 288 AA.
AC F4IMQ0; O64735; Q93ZA1;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=Protein FLC EXPRESSOR;
DE Short=AtFLX;
DE AltName: Full=Protein SUPPRESSOR OF FRI 5;
GN Name=FLX; Synonyms=SUF5; OrderedLocusNames=At2g30120; ORFNames=E13.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, ALTERNATIVE SPLICING, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY,
RP AND SUBCELLULAR LOCATION.
RC STRAIN=cv. C24;
RX PubMed=18156133; DOI=10.1093/pcp/pcm176;
RA Andersson C.R., Helliwell C.A., Bagnall D.J., Hughes T.P., Finnegan E.J.,
RA Peacock W.J., Dennis E.S.;
RT "The FLX gene of Arabidopsis is required for FRI-dependent activation of
RT FLC expression.";
RL Plant Cell Physiol. 49:191-200(2008).
RN [6]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY IN THE FRI-C COMPLEX,
RP INTERACTION WITH FRI; SWC6; TAF14B; RIN1 AND FES1, TISSUE SPECIFICITY, AND
RP SUBCELLULAR LOCATION.
RX PubMed=21282526; DOI=10.1105/tpc.110.075911;
RA Choi K., Kim J., Hwang H.J., Kim S., Park C., Kim S.Y., Lee I.;
RT "The FRIGIDA complex activates transcription of FLC, a strong flowering
RT repressor in Arabidopsis, by recruiting chromatin modification factors.";
RL Plant Cell 23:289-303(2011).
RN [7]
RP FUNCTION, AND MUTAGENESIS OF ARG-176.
RX PubMed=23864009; DOI=10.1038/ncomms3186;
RA Lee J., Amasino R.M.;
RT "Two FLX family members are non-redundantly required to establish the
RT vernalization requirement in Arabidopsis.";
RL Nat. Commun. 4:2186-2186(2013).
CC -!- FUNCTION: Transactivator required for FRIGIDA-mediated activation of
CC FLC and for the expression of the repressors of flowering AGL27/MAF1
CC and AGL31/MAF2. Does not alter expression of known activators of FLC
CC expression. Not required for the photoperiod response or for the
CC vernalization response. Not redundant with FLXL4.
CC {ECO:0000269|PubMed:18156133, ECO:0000269|PubMed:21282526,
CC ECO:0000269|PubMed:23864009}.
CC -!- SUBUNIT: Homodimer. Component of the transcription activator complex
CC FRI-C composed of FRI, FRL1, SUF4, FLX and FES1. Interacts with FES1
CC and (via N-terminus) with FRI (via C-terminus). Interacts with SWC6,
CC TAF14B and RIN1, three components of the SWR1 chromatin-remodeling
CC complex. {ECO:0000269|PubMed:21282526}.
CC -!- INTERACTION:
CC F4IMQ0; Q9LEZ3: BIM1; NbExp=3; IntAct=EBI-4447281, EBI-617095;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18156133,
CC ECO:0000269|PubMed:21282526}. Nucleus {ECO:0000269|PubMed:18156133,
CC ECO:0000269|PubMed:21282526}. Note=homodimers present in the cytosol
CC and heterodimers with FRI and FES1 detected only in the nucleus.
CC {ECO:0000269|PubMed:21282526}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4IMQ0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4IMQ0-2; Sequence=VSP_053267;
CC -!- TISSUE SPECIFICITY: Expressed in roots and leaves. Expressed in shoot
CC and root apical meristems and vascular tissues.
CC {ECO:0000269|PubMed:18156133, ECO:0000269|PubMed:21282526}.
CC -!- DISRUPTION PHENOTYPE: Early flowering. Decreased level of H3K4me3 and
CC histone H3 and H4 acetylation in the region spanning the transcription-
CC translation start site of FLC, but not of UFC or DFC.
CC {ECO:0000269|PubMed:18156133}.
CC -!- SIMILARITY: Belongs to the FLX family. {ECO:0000305}.
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DR EMBL; AC004165; AAC16960.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC08347.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08348.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62825.1; -; Genomic_DNA.
DR EMBL; AY057695; AAL15325.1; -; mRNA.
DR EMBL; AY116952; AAM51586.1; -; mRNA.
DR EMBL; AK221657; BAD95329.1; -; mRNA.
DR PIR; T00586; T00586.
DR RefSeq; NP_001154541.1; NM_001161069.1. [F4IMQ0-1]
DR RefSeq; NP_001324953.1; NM_001336242.1. [F4IMQ0-2]
DR RefSeq; NP_565694.1; NM_128567.3. [F4IMQ0-2]
DR AlphaFoldDB; F4IMQ0; -.
DR SMR; F4IMQ0; -.
DR BioGRID; 2913; 13.
DR IntAct; F4IMQ0; 5.
DR STRING; 3702.AT2G30120.2; -.
DR PaxDb; F4IMQ0; -.
DR EnsemblPlants; AT2G30120.1; AT2G30120.1; AT2G30120. [F4IMQ0-2]
DR EnsemblPlants; AT2G30120.2; AT2G30120.2; AT2G30120. [F4IMQ0-1]
DR EnsemblPlants; AT2G30120.6; AT2G30120.6; AT2G30120. [F4IMQ0-2]
DR GeneID; 817564; -.
DR Gramene; AT2G30120.1; AT2G30120.1; AT2G30120. [F4IMQ0-2]
DR Gramene; AT2G30120.2; AT2G30120.2; AT2G30120. [F4IMQ0-1]
DR Gramene; AT2G30120.6; AT2G30120.6; AT2G30120. [F4IMQ0-2]
DR KEGG; ath:AT2G30120; -.
DR Araport; AT2G30120; -.
DR TAIR; locus:2060848; AT2G30120.
DR eggNOG; ENOG502RJEI; Eukaryota.
DR HOGENOM; CLU_051930_4_1_1; -.
DR InParanoid; F4IMQ0; -.
DR OMA; ITMTAEI; -.
DR PRO; PR:F4IMQ0; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IMQ0; baseline and differential.
DR Genevisible; F4IMQ0; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR InterPro; IPR040353; FLX/FLX-like.
DR PANTHER; PTHR33405; PTHR33405; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Coiled coil; Cytoplasm;
KW Developmental protein; Differentiation; Flowering; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..288
FT /note="Protein FLC EXPRESSOR"
FT /id="PRO_0000423734"
FT REGION 225..257
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 38..94
FT /evidence="ECO:0000255"
FT COILED 132..231
FT /evidence="ECO:0000255"
FT VAR_SEQ 177..288
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172, ECO:0000303|Ref.4"
FT /id="VSP_053267"
FT MUTAGEN 176
FT /note="R->K: In flx-3; early flowering."
FT /evidence="ECO:0000269|PubMed:23864009"
SQ SEQUENCE 288 AA; 32086 MW; 123F1678A852DD96 CRC64;
MAGRDRYIPS SAVSTSSSSR LLESQLIESD RNRARSVILE DRIAIQHREI QSLLNDNQRL
AVAHIGLKDQ LNVAKRELER LLETAVKVKA EGEAKVREVY QNALRMEAEA RVIDGLGAEL
GQVRSDVQRL GSDRQELATE LAMFDDEMAK AKPNSDRAIE VKLEIEILRG EIRKGRAALE
LEKKTRASNL HHERGMEKTI DHLNREIVKL EEELVDLETK AREANAAAEA APTPSPGLAA
SYGNNTDDIY GGQGRQYPEA NGTHELVLRE KSYVHRLVSV QLVQVSVG
