ID   FMAH_DICNO              Reviewed;         156 AA.
AC   P04953;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Type IV major fimbrial protein FimA;
DE   AltName: Full=265 antigen;
DE   AltName: Full=Pilin;
DE   AltName: Full=Serogroup H1;
DE   AltName: Full=Two subunits pilin;
DE   Contains:
DE     RecName: Full=Pilin subunit 1;
DE   Contains:
DE     RecName: Full=Pilin subunit 2;
DE   Flags: Precursor;
GN   Name=fimA;
OS   Dichelobacter nodosus (Bacteroides nodosus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Cardiobacteriales;
OC   Cardiobacteriaceae; Dichelobacter.
OX   NCBI_TaxID=870;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Serogroup H isolate 265;
RX   PubMed=2873127; DOI=10.1128/jb.167.1.243-250.1986;
RA   Elleman T.C., Hoyne P.A., McKern N.M., Stewart D.J.;
RT   "Nucleotide sequence of the gene encoding the two-subunit pilin of
RT   Bacteroides nodosus 265.";
RL   J. Bacteriol. 167:243-250(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Serogroup H1 isolate VCS1215;
RX   PubMed=1675419; DOI=10.1111/j.1365-2958.1991.tb00727.x;
RA   Mattick J.S., Anderson B.J., Cox P.T., Dalrymple B.P., Bills M.M.,
RA   Hobbs M., Egerton J.R.;
RT   "Gene sequences and comparison of the fimbrial subunits representative of
RT   Bacteroides nodosus serotypes A to I: class I and class II strains.";
RL   Mol. Microbiol. 5:561-573(1991).
CC   -!- FUNCTION: Major component of the type IV fimbriae that plays an
CC       essential role in twitching motility, natural transformation, and
CC       protease secretion. {ECO:0000250|UniProtKB:A5EWR9}.
CC   -!- SUBUNIT: The pili are polar flexible filaments of about 5.4 nanometers
CC       diameter and 2.5 micrometers average length; they consist of only a
CC       single polypeptide chain arranged in a helical configuration of five
CC       subunits per turn in the assembled pilus.
CC   -!- SUBCELLULAR LOCATION: Fimbrium {ECO:0000250|UniProtKB:A5EWR9}. Membrane
CC       {ECO:0000255}; Single-pass membrane protein {ECO:0000255}.
CC   -!- MISCELLANEOUS: The sequence shown is that of isolate 265.
CC   -!- SIMILARITY: Belongs to the N-Me-Phe pilin family. {ECO:0000305}.
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DR   EMBL; M13765; AAA23346.1; -; Genomic_DNA.
DR   EMBL; X52390; CAA36619.1; -; Genomic_DNA.
DR   PIR; S15267; S15267.
DR   AlphaFoldDB; P04953; -.
DR   SMR; P04953; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009289; C:pilus; IEA:UniProtKB-SubCell.
DR   GO; GO:0015627; C:type II protein secretion system complex; IEA:InterPro.
DR   GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR   GO; GO:0015628; P:protein secretion by the type II secretion system; IEA:InterPro.
DR   InterPro; IPR000983; Bac_GSPG_pilin.
DR   InterPro; IPR012902; N_methyl_site.
DR   InterPro; IPR001082; Pilin.
DR   InterPro; IPR045584; Pilin-like.
DR   Pfam; PF07963; N_methyl; 1.
DR   Pfam; PF00114; Pilin; 1.
DR   PRINTS; PR00813; BCTERIALGSPG.
DR   SUPFAM; SSF54523; SSF54523; 1.
DR   TIGRFAMs; TIGR02532; IV_pilin_GFxxxE; 1.
DR   PROSITE; PS00409; PROKAR_NTER_METHYL; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Fimbrium; Membrane; Methylation; Transmembrane;
KW   Transmembrane helix.
FT   PROPEP          1..7
FT                   /note="Leader sequence"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01070"
FT                   /id="PRO_0000024137"
FT   CHAIN           8..79
FT                   /note="Pilin subunit 1"
FT                   /id="PRO_0000024138"
FT   CHAIN           80..156
FT                   /note="Pilin subunit 2"
FT                   /id="PRO_0000024139"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         8
FT                   /note="N-methylphenylalanine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01070"
FT   DISULFID        57..67
FT   DISULFID        141..154
FT   VARIANT         91
FT                   /note="A -> E (in strain: Serogroup H1 isolate VCS1215)"
FT   VARIANT         105
FT                   /note="E -> G (in strain: Serogroup H1 isolate VCS1215)"
FT   VARIANT         156
FT                   /note="K -> Q (in strain: Serogroup H1 isolate VCS1215)"
SQ   SEQUENCE   156 AA;  16637 MW;  7AB398C25AF9B6A6 CRC64;
     MKSLQKGFTL IELMIVVAII GILAAIAIPQ YQNYIARSQV SRVMSETGQM RTAIETCLLD
     GKEGKDCFIG WTTSNLLAAA GGSTTNNATA ADPGQGGLNI TYALESTAEN KIEATFGQNA
     AATLHGKKLT WTRSPEATWS CSTDVDEKFK PTGCKK