ID   FMDA_METME              Reviewed;         407 AA.
AC   Q50228;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Formamidase;
DE            EC=3.5.1.49;
DE   AltName: Full=Formamide amidohydrolase;
GN   Name=fmdA;
OS   Methylophilus methylotrophus (Bacterium W3A1).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Methylophilaceae; Methylophilus.
OX   NCBI_TaxID=17;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, AND MASS
RP   SPECTROMETRY.
RC   STRAIN=ATCC 53528 / AS1 / DSM 46235 / LMG 6787 / NCIMB 10515;
RX   PubMed=8841393; DOI=10.1111/j.1432-1033.1996.0314h.x;
RA   Wyborn N.R., Mills J., Williams S.G., Jones C.W.;
RT   "Molecular characterisation of formamidase from Methylophilus
RT   methylotrophus.";
RL   Eur. J. Biochem. 240:314-322(1996).
RN   [2]
RP   CHARACTERIZATION, AND PROTEIN SEQUENCE OF 1-5.
RC   STRAIN=ATCC 53528 / AS1 / DSM 46235 / LMG 6787 / NCIMB 10515;
RA   Wyborn N.R., Scherr D.J., Jones C.W.;
RT   "Purification, properties and heterologous expression of formamidase from
RT   Methylophilus methylotrophus.";
RL   Microbiology 140:191-195(1994).
CC   -!- FUNCTION: Hydrolyzes formamide with the production of ammonia which can
CC       be used as a source of nitrogen for growth. Also acts, more slowly, on
CC       acetamide, propanamide and butanamide.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=formamide + H2O = formate + NH4(+); Xref=Rhea:RHEA:21948,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15740, ChEBI:CHEBI:16397,
CC         ChEBI:CHEBI:28938; EC=3.5.1.49;
CC   -!- SUBUNIT: Homotrimer.
CC   -!- MASS SPECTROMETRY: Mass=44481; Mass_error=26; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:8841393};
CC   -!- SIMILARITY: Belongs to the acetamidase/formamidase family.
CC       {ECO:0000305}.
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DR   EMBL; X99632; CAA67953.1; -; Genomic_DNA.
DR   PIR; S74213; S74213.
DR   AlphaFoldDB; Q50228; -.
DR   SMR; Q50228; -.
DR   STRING; 1122236.KB905141_gene1523; -.
DR   GO; GO:0004328; F:formamidase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR004304; FmdA_AmdA.
DR   PANTHER; PTHR31891; PTHR31891; 1.
DR   Pfam; PF03069; FmdA_AmdA; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase.
FT   CHAIN           1..407
FT                   /note="Formamidase"
FT                   /id="PRO_0000087312"
SQ   SEQUENCE   407 AA;  44466 MW;  C55E888BA4C44A70 CRC64;
     MKTIVKLDLD KKPWEQDGQI HNRWHPDLPM IAMVKPGDEF RVECMDWTGG QIGNNDSAND
     VRDVDLTQVH YLSGPIGVEG AEPGDLMVVD ILDVGTFDDS QWGFNGLFAK ENGGGFLTDH
     FPEASKTIWD FHGVYTTSRH VPKVRYAGIM HPGLIGCLPS KELLDTWNKR EGDLIATDPD
     RVPPLACPPT SQSAVMGRLS GDAAKKAAAE GARTVPPRDH GGNCDIKNLT KGSRVYFPVY
     VKDGGLSMGD LHFSQGDGEI TFCGAIEMAG YLDIKVGLIK DGVKKYGIKN PVFQPSPITP
     TYRDYMIFEG ISVDEAGKQH YLDVHIAYRQ ACLNAIEYLK KFGYSGEQAV SILGTAPVEG
     HISGIVDIPN ACATLWIPTE IFEFDIRPNA DGPKIMVPPG VDVSFTS