FMDC_METTH
ID FMDC_METTH Reviewed; 400 AA.
AC O27002;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Molybdenum-containing formylmethanofuran dehydrogenase 1 subunit C;
DE EC=1.2.7.12 {ECO:0000250|UniProtKB:Q48943};
DE AltName: Full=Molybdenum-containing formylmethanofuran dehydrogenase I subunit C;
GN Name=fmdC; OrderedLocusNames=MTH_918;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
CC -!- FUNCTION: Catalyzes the reversible oxidation of CO(2) and methanofuran
CC (MFR) to N-formylmethanofuran (CHO-MFR). Can only oxidize
CC formylmethanofuran. This enzyme is oxygen-labile.
CC {ECO:0000250|UniProtKB:Q48943}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + N-formylmethanofuran + 2 oxidized [2Fe-2S]-[ferredoxin]
CC = CO2 + H(+) + methanofuran + 2 reduced [2Fe-2S]-[ferredoxin];
CC Xref=Rhea:RHEA:19841, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57727,
CC ChEBI:CHEBI:58151; EC=1.2.7.12;
CC Evidence={ECO:0000250|UniProtKB:Q48943};
CC -!- ACTIVITY REGULATION: Inactivated by cyanide.
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.
CC -!- SUBUNIT: Consists of five subunits; FmdA, FmdB, FmdC, FmdD, and FmdE.
CC -!- INDUCTION: By growth on molybdenum, under anaerobic conditions.
CC -!- SIMILARITY: In the N-terminal section; belongs to the FwdC/FmdC family.
CC {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the molybdenum
CC dinucleotide binding protein family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE000666; AAB85416.1; -; Genomic_DNA.
DR PIR; H69222; H69222.
DR RefSeq; WP_010876551.1; NC_000916.1.
DR AlphaFoldDB; O27002; -.
DR SMR; O27002; -.
DR STRING; 187420.MTH_918; -.
DR EnsemblBacteria; AAB85416; AAB85416; MTH_918.
DR GeneID; 1471326; -.
DR KEGG; mth:MTH_918; -.
DR PATRIC; fig|187420.15.peg.903; -.
DR HOGENOM; CLU_661592_0_0_2; -.
DR OMA; SSWIGME; -.
DR BioCyc; MetaCyc:FMDCMAUTO-MON; -.
DR UniPathway; UPA00640; UER00692.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0018493; F:formylmethanofuran dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0030151; F:molybdenum ion binding; IEA:InterPro.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR CDD; cd00980; FwdC/FmdC; 1.
DR CDD; cd02789; MopB_CT_FmdC-FwdD; 1.
DR Gene3D; 2.160.20.60; -; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR041717; FmdC/FwdD_MopB-bd.
DR InterPro; IPR012048; Formylmethanofuran_DH_csu/dsu.
DR InterPro; IPR017550; Formylmethanofuran_DH_suC.
DR InterPro; IPR036485; Glu_synth_asu_C_sf.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR Pfam; PF01568; Molydop_binding; 1.
DR PIRSF; PIRSF036633; FmdC_D; 1.
DR SUPFAM; SSF50692; SSF50692; 1.
DR SUPFAM; SSF69336; SSF69336; 1.
DR TIGRFAMs; TIGR03122; one_C_dehyd_C; 1.
PE 2: Evidence at transcript level;
KW Methanogenesis; Oxidoreductase; Reference proteome; Repeat.
FT CHAIN 1..400
FT /note="Molybdenum-containing formylmethanofuran
FT dehydrogenase 1 subunit C"
FT /id="PRO_0000144192"
FT REPEAT 76..88
FT /note="1"
FT REPEAT 95..107
FT /note="2"
FT REPEAT 114..126
FT /note="3"
FT REPEAT 140..152
FT /note="4"
FT REPEAT 159..171
FT /note="5"
FT REPEAT 178..190
FT /note="6"
FT REPEAT 197..209
FT /note="7"
FT REGION 76..209
FT /note="7 X 13 AA repeats of [GW]-X-X-M-X-X-G-X-I-X-[IV]-X-
FT G"
SQ SEQUENCE 400 AA; 43288 MW; DBC32FF73A5DDF05 CRC64;
MGFVLVPKSD FQIPLEADTI RPDLFEGLDL DEIRSLQVYE GNIKRPLGEF FEIAETPHAD
QLIRIDGDVS RVKYIGSGMK SGKIIINGDV GLQLGCEMKG GEIEVNGNVS SWIGMEMHGG
TIKINGNAGD YVGCAYRGEW RGMKGGKIII QGNAGNNIGG GMMAGEIYIG GDAGNFCGIR
MNGGEITVRG DAGRAPGAEM VSGIIKIHGR ISSLLPGFKE ISTFKEDGSL MILFKGDLSE
KNPEGNLYIN YNKNLHILEN ETDEGRVITK KGIKVIYNSG STIREGQIIK GGNKLTDDYI
DECARCCISP EDYKLLGEPE NVVVSSHGNE VVLRAVEDPG IQMGTIFIPR GIWANVLTPP
YTESTGSPMY KGVPVYLRKA SQGERILSAE ELVEEYGVGK
