FMRF_APLCA
ID FMRF_APLCA Reviewed; 597 AA.
AC P08021; P08020; P08022; Q16963; Q16964; Q16965;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 3.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=FMRF-amide neuropeptides;
DE Contains:
DE RecName: Full=FLRF-amide;
DE Contains:
DE RecName: Full=FMRF-amide;
DE Flags: Precursor;
OS Aplysia californica (California sea hare).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Heterobranchia; Euthyneura; Tectipleura; Aplysiida; Aplysioidea;
OC Aplysiidae; Aplysia.
OX NCBI_TaxID=6500;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND ALTERNATIVE SPLICING.
RX PubMed=3816495; DOI=10.1089/dna.1.1986.5.453;
RA Taussig R., Scheller R.H.;
RT "The Aplysia FMRFamide gene encodes sequences related to mammalian brain
RT peptides.";
RL DNA 5:453-461(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 154-597 (ISOFORM 3).
RX PubMed=3838698; DOI=10.1016/s0092-8674(85)80019-2;
RA Schaefer M., Picciotto M.R., Kreiner T., Kaldany R.-R., Taussig R.,
RA Scheller R.H.;
RT "Aplysia neurons express a gene encoding multiple FMRFamide
RT neuropeptides.";
RL Cell 41:457-467(1985).
RN [3]
RP MASS SPECTROMETRY, AND AMIDATION AT PHE-72; PHE-156; PHE-163; PHE-201;
RP PHE-208; PHE-223; PHE-239; PHE-246; PHE-262; PHE-277; PHE-292; PHE-307;
RP PHE-322; PHE-337; PHE-352; PHE-367; PHE-382; PHE-397; PHE-413; PHE-429;
RP PHE-445; PHE-461; PHE-477; PHE-493; PHE-509; PHE-525; PHE-541; PHE-557 AND
RP PHE-564.
RX PubMed=9809658; DOI=10.1016/s0196-9781(98)00094-1;
RA Li L., Moroz T.P., Garden R.W., Floyd P.D., Weiss K.R., Sweedler J.V.;
RT "Mass spectrometric survey of interganglionically transported peptides in
RT Aplysia.";
RL Peptides 19:1425-1433(1998).
CC -!- FUNCTION: FMRFamide induces contractions in visceral and somatic
CC musculature as well as in the heart.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=P08021-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P08021-2; Sequence=VSP_029897, VSP_029898;
CC Name=3;
CC IsoId=P08021-3; Sequence=VSP_029899;
CC -!- MASS SPECTROMETRY: [FLRF-amide]: Mass=580.43; Mass_error=0.10;
CC Method=MALDI; Note=FLRF-amide.; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [FMRF-amide]: Mass=598.38; Mass_error=0.09;
CC Method=MALDI; Note=FMRF-amide.; Evidence={ECO:0000269|PubMed:9809658};
CC -!- SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L29473; AAB59170.1; -; mRNA.
DR EMBL; M11282; AAA27751.1; -; mRNA.
DR EMBL; M11283; AAB59169.1; -; mRNA.
DR EMBL; M14956; AAA27754.1; -; mRNA.
DR EMBL; M14958; AAA27755.1; -; Genomic_DNA.
DR EMBL; M14960; AAA27752.1; -; mRNA.
DR EMBL; M14961; AAA27753.1; -; mRNA.
DR PIR; A23172; A23172.
DR RefSeq; NP_001191475.1; NM_001204546.1.
DR AlphaFoldDB; P08021; -.
DR PRIDE; P08021; -.
DR GeneID; 100533232; -.
DR OrthoDB; 975215at2759; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR002544; FMRFamid-related_peptide-like.
DR Pfam; PF01581; FARP; 30.
PE 1: Evidence at protein level;
KW Alternative splicing; Amidation; Cleavage on pair of basic residues;
KW Neuropeptide; Repeat; Secreted.
FT PROPEP 1..68
FT /note="Removed in mature form"
FT /evidence="ECO:0000305"
FT /id="PRO_0000441101"
FT PEPTIDE 69..72
FT /note="FLRF-amide"
FT /id="PRO_0000009626"
FT PROPEP 75..150
FT /evidence="ECO:0000250"
FT /id="PRO_0000288252"
FT PEPTIDE 153..156
FT /note="FMRF-amide"
FT /id="PRO_0000009627"
FT PEPTIDE 160..163
FT /note="FMRF-amide"
FT /id="PRO_0000009628"
FT PROPEP 166..195
FT /evidence="ECO:0000250"
FT /id="PRO_0000288253"
FT PEPTIDE 198..201
FT /note="FMRF-amide"
FT /id="PRO_0000009629"
FT PEPTIDE 205..208
FT /note="FMRF-amide"
FT /id="PRO_0000009630"
FT PROPEP 212..217
FT /evidence="ECO:0000250"
FT /id="PRO_0000288254"
FT PEPTIDE 220..223
FT /note="FMRF-amide"
FT /id="PRO_0000009631"
FT PROPEP 226..233
FT /evidence="ECO:0000250"
FT /id="PRO_0000288255"
FT PEPTIDE 236..239
FT /note="FMRF-amide"
FT /id="PRO_0000009632"
FT PEPTIDE 243..246
FT /note="FMRF-amide"
FT /id="PRO_0000009633"
FT PROPEP 249..257
FT /evidence="ECO:0000250"
FT /id="PRO_0000288256"
FT PEPTIDE 259..262
FT /note="FMRF-amide"
FT /id="PRO_0000009634"
FT PROPEP 265..271
FT /evidence="ECO:0000250"
FT /id="PRO_0000288257"
FT PEPTIDE 274..277
FT /note="FMRF-amide"
FT /id="PRO_0000009635"
FT PROPEP 280..286
FT /evidence="ECO:0000250"
FT /id="PRO_0000288258"
FT PEPTIDE 289..292
FT /note="FMRF-amide"
FT /id="PRO_0000009636"
FT PROPEP 295..301
FT /evidence="ECO:0000250"
FT /id="PRO_0000288259"
FT PEPTIDE 304..307
FT /note="FMRF-amide"
FT /id="PRO_0000009637"
FT PROPEP 310..316
FT /evidence="ECO:0000250"
FT /id="PRO_0000288260"
FT PEPTIDE 319..322
FT /note="FMRF-amide"
FT /id="PRO_0000009638"
FT PROPEP 325..331
FT /evidence="ECO:0000250"
FT /id="PRO_0000288261"
FT PEPTIDE 334..337
FT /note="FMRF-amide"
FT /id="PRO_0000009639"
FT PROPEP 340..346
FT /evidence="ECO:0000250"
FT /id="PRO_0000288262"
FT PEPTIDE 349..352
FT /note="FMRF-amide"
FT /id="PRO_0000009640"
FT PROPEP 355..361
FT /evidence="ECO:0000250"
FT /id="PRO_0000288263"
FT PEPTIDE 364..367
FT /note="FMRF-amide"
FT /id="PRO_0000009641"
FT PROPEP 370..376
FT /evidence="ECO:0000250"
FT /id="PRO_0000288264"
FT PEPTIDE 379..382
FT /note="FMRF-amide"
FT /id="PRO_0000009642"
FT PROPEP 385..391
FT /evidence="ECO:0000250"
FT /id="PRO_0000288265"
FT PEPTIDE 394..397
FT /note="FMRF-amide"
FT /id="PRO_0000009643"
FT PROPEP 400..407
FT /evidence="ECO:0000250"
FT /id="PRO_0000288266"
FT PEPTIDE 410..413
FT /note="FMRF-amide"
FT /id="PRO_0000009644"
FT PROPEP 416..423
FT /evidence="ECO:0000250"
FT /id="PRO_0000288267"
FT PEPTIDE 426..429
FT /note="FMRF-amide"
FT /id="PRO_0000009645"
FT PROPEP 432..439
FT /evidence="ECO:0000250"
FT /id="PRO_0000288268"
FT PEPTIDE 442..445
FT /note="FMRF-amide"
FT /id="PRO_0000009646"
FT PROPEP 448..455
FT /evidence="ECO:0000250"
FT /id="PRO_0000288269"
FT PEPTIDE 458..461
FT /note="FMRF-amide"
FT /id="PRO_0000009647"
FT PROPEP 464..471
FT /evidence="ECO:0000250"
FT /id="PRO_0000288270"
FT PEPTIDE 474..477
FT /note="FMRF-amide"
FT /id="PRO_0000009648"
FT PROPEP 480..487
FT /evidence="ECO:0000250"
FT /id="PRO_0000288271"
FT PEPTIDE 490..493
FT /note="FMRF-amide"
FT /id="PRO_0000009649"
FT PROPEP 496..503
FT /evidence="ECO:0000250"
FT /id="PRO_0000288272"
FT PEPTIDE 506..509
FT /note="FMRF-amide"
FT /id="PRO_0000009650"
FT PROPEP 512..519
FT /evidence="ECO:0000250"
FT /id="PRO_0000288273"
FT PEPTIDE 522..525
FT /note="FMRF-amide"
FT /id="PRO_0000009651"
FT PROPEP 528..535
FT /evidence="ECO:0000250"
FT /id="PRO_0000288274"
FT PEPTIDE 538..541
FT /note="FMRF-amide"
FT /id="PRO_0000009652"
FT PROPEP 544..551
FT /evidence="ECO:0000250"
FT /id="PRO_0000288275"
FT PEPTIDE 554..557
FT /note="FMRF-amide"
FT /id="PRO_0000009653"
FT PEPTIDE 561..564
FT /note="FMRF-amide"
FT /id="PRO_0000009654"
FT PROPEP 567..597
FT /evidence="ECO:0000250"
FT /id="PRO_0000288276"
FT REGION 98..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 569..597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 571..597
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 72
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 156
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 163
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 201
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 208
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 223
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 239
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 246
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 262
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 277
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 292
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 307
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 322
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 337
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 352
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 367
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 382
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 397
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 413
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 429
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 445
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 461
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 477
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 493
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 509
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 525
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 541
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 557
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT MOD_RES 564
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:9809658"
FT VAR_SEQ 156..200
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_029897"
FT VAR_SEQ 262..351
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_029898"
FT VAR_SEQ 313..405
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:3838698"
FT /id="VSP_029899"
FT CONFLICT 112
FT /note="V -> L (in Ref. 1; AAA27754)"
FT /evidence="ECO:0000305"
FT CONFLICT 173
FT /note="S -> N (in Ref. 1; AAA27754)"
FT /evidence="ECO:0000305"
FT CONFLICT 299
FT /note="D -> G (in Ref. 1; AAA27752)"
FT /evidence="ECO:0000305"
FT CONFLICT 327
FT /note="D -> G (in Ref. 1; AAA27753)"
FT /evidence="ECO:0000305"
FT CONFLICT 421
FT /note="E -> V (in Ref. 1; AAA27752)"
FT /evidence="ECO:0000305"
FT CONFLICT 439
FT /note="N -> D (in Ref. 1; AAA27751/AAA27752/AAA27753)"
FT /evidence="ECO:0000305"
FT CONFLICT 455
FT /note="N -> D (in Ref. 1; AAA27751/AAA27752/AAA27753)"
FT /evidence="ECO:0000305"
FT CONFLICT 481
FT /note="L -> V (in Ref. 1; AAB59170)"
FT /evidence="ECO:0000305"
FT CONFLICT 483
FT /note="T -> S (in Ref. 1; AAB59170)"
FT /evidence="ECO:0000305"
FT CONFLICT 485
FT /note="D -> E (in Ref. 1; AAB59170)"
FT /evidence="ECO:0000305"
FT CONFLICT 497
FT /note="L -> V (in Ref. 1; AAB59170)"
FT /evidence="ECO:0000305"
FT CONFLICT 498
FT /note="G -> E (in Ref. 1; AAA27752)"
FT /evidence="ECO:0000305"
FT CONFLICT 503
FT /note="D -> N (in Ref. 1; AAA27752)"
FT /evidence="ECO:0000305"
FT CONFLICT 516
FT /note="E -> D (in Ref. 1; AAB59170)"
FT /evidence="ECO:0000305"
FT CONFLICT 532
FT /note="D -> E (in Ref. 1; AAB59170)"
FT /evidence="ECO:0000305"
FT CONFLICT 575
FT /note="S -> G (in Ref. 1; AAA27751/AAB59170)"
FT /evidence="ECO:0000305"
FT CONFLICT 580
FT /note="S -> N (in Ref. 1; AAA27751/AAA27752/AAB59170)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 597 AA; 69630 MW; EE1386461CEF6EAF CRC64;
MRPWCQLALL ACLSLKWLTS HVTAESFLCD DSELCENGYL RFGRSMSVEE PHFRLERRSY
PPVVYHKRFL RFGRSQEPDI EDYARAIALI ESEEPLYRKR RSADADGQSE KVLHRARREA
ESEHKSLEEV SPDTKQDVEK RDADDVLDAE KRFMRFGKRF MRFGRGSSDD DESGDDDVQD
LTDIGDGLGG EGEVNKRFMR FGKRFMRFGK REDGEPDKRF MRFGKSMADN DLDKRFMRFG
KRFMRFGKSL PDSEVDKRFM RFGKSVDGDV DKRFMRFGKS VDGDVDKRFM RFGKSVDGDV
DKRFMRFGKS VDGDVDKRFM RFGKSVDGDV DKRFMRFGKS VDDDVDKRFM RFGKSVDGDV
DKRFMRFGKS VDDAVDKRFM RFGKSVDSDL DKRFMRFGKS VGSDEVDKRF MRFGKSVGSD
EVDKRFMRFG KSLGTDDVNK RFMRFGKSLG TDDVNKRFMR FGKSLGTEDV DKRFMRFGKS
LGTDDVDKRF MRFGKSLGTD DVDKRFMRFG KSLGTEDVDK RFMRFGKSLG TDDVDKRFMR
FGKSLGTEDV DKRFMRFGKR FMRFGRSVGD SKYRSASSES VMTTDSKQTT EQATNKS
