FMT_BACAN
ID FMT_BACAN Reviewed; 314 AA.
AC Q81WH2; Q6HUM0; Q6KNV4;
DT 04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Methionyl-tRNA formyltransferase {ECO:0000255|HAMAP-Rule:MF_00182};
DE EC=2.1.2.9 {ECO:0000255|HAMAP-Rule:MF_00182};
GN Name=fmt {ECO:0000255|HAMAP-Rule:MF_00182};
GN OrderedLocusNames=BA_4004, GBAA_4004, BAS3717;
OS Bacillus anthracis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1392;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames / isolate Porton;
RX PubMed=12721629; DOI=10.1038/nature01586;
RA Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C.,
RA Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y.,
RA Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M.,
RA Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E.,
RA White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT "The genome sequence of Bacillus anthracis Ames and comparison to closely
RT related bacteria.";
RL Nature 423:81-86(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames ancestor;
RX PubMed=18952800; DOI=10.1128/jb.01347-08;
RA Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL J. Bacteriol. 191:445-446(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sterne;
RA Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA Richardson P., Rubin E., Tice H.;
RT "Complete genome sequence of Bacillus anthracis Sterne.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Attaches a formyl group to the free amino group of methionyl-
CC tRNA(fMet). The formyl group appears to play a dual role in the
CC initiator identity of N-formylmethionyl-tRNA by promoting its
CC recognition by IF2 and preventing the misappropriation of this tRNA by
CC the elongation apparatus. {ECO:0000255|HAMAP-Rule:MF_00182}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) =
CC (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-
CC tRNA(fMet); Xref=Rhea:RHEA:24380, Rhea:RHEA-COMP:9952, Rhea:RHEA-
CC COMP:9953, ChEBI:CHEBI:15378, ChEBI:CHEBI:57453, ChEBI:CHEBI:57454,
CC ChEBI:CHEBI:78530, ChEBI:CHEBI:78844; EC=2.1.2.9;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00182};
CC -!- SIMILARITY: Belongs to the Fmt family. {ECO:0000255|HAMAP-
CC Rule:MF_00182}.
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DR EMBL; AE016879; AAP27732.1; -; Genomic_DNA.
DR EMBL; AE017334; AAT33120.1; -; Genomic_DNA.
DR EMBL; AE017225; AAT56019.1; -; Genomic_DNA.
DR RefSeq; NP_846246.1; NC_003997.3.
DR RefSeq; WP_000598790.1; NZ_WXXJ01000026.1.
DR RefSeq; YP_029968.1; NC_005945.1.
DR PDB; 4IQF; X-ray; 2.40 A; A/B/C/D=1-314.
DR PDBsum; 4IQF; -.
DR AlphaFoldDB; Q81WH2; -.
DR SMR; Q81WH2; -.
DR STRING; 260799.BAS3717; -.
DR DNASU; 1086742; -.
DR EnsemblBacteria; AAP27732; AAP27732; BA_4004.
DR EnsemblBacteria; AAT33120; AAT33120; GBAA_4004.
DR GeneID; 45023695; -.
DR KEGG; ban:BA_4004; -.
DR KEGG; bar:GBAA_4004; -.
DR KEGG; bat:BAS3717; -.
DR PATRIC; fig|198094.11.peg.3974; -.
DR eggNOG; COG0223; Bacteria.
DR HOGENOM; CLU_033347_1_1_9; -.
DR OMA; CCPVVAY; -.
DR Proteomes; UP000000427; Chromosome.
DR Proteomes; UP000000594; Chromosome.
DR GO; GO:0004479; F:methionyl-tRNA formyltransferase activity; IEA:UniProtKB-UniRule.
DR CDD; cd08646; FMT_core_Met-tRNA-FMT_N; 1.
DR CDD; cd08704; Met_tRNA_FMT_C; 1.
DR Gene3D; 3.10.25.10; -; 1.
DR HAMAP; MF_00182; Formyl_trans; 1.
DR InterPro; IPR005794; Fmt.
DR InterPro; IPR005793; Formyl_trans_C.
DR InterPro; IPR037022; Formyl_trans_C_sf.
DR InterPro; IPR002376; Formyl_transf_N.
DR InterPro; IPR036477; Formyl_transf_N_sf.
DR InterPro; IPR011034; Formyl_transferase-like_C_sf.
DR InterPro; IPR001555; GART_AS.
DR InterPro; IPR044135; Met-tRNA-FMT_C.
DR InterPro; IPR041711; Met-tRNA-FMT_N.
DR Pfam; PF02911; Formyl_trans_C; 1.
DR Pfam; PF00551; Formyl_trans_N; 1.
DR SUPFAM; SSF50486; SSF50486; 1.
DR SUPFAM; SSF53328; SSF53328; 1.
DR TIGRFAMs; TIGR00460; fmt; 1.
DR PROSITE; PS00373; GART; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Protein biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..314
FT /note="Methionyl-tRNA formyltransferase"
FT /id="PRO_0000082910"
FT BINDING 110..113
FT /ligand="(6S)-5,6,7,8-tetrahydrofolate"
FT /ligand_id="ChEBI:CHEBI:57453"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00182"
FT STRAND 3..8
FT /evidence="ECO:0007829|PDB:4IQF"
FT HELIX 13..22
FT /evidence="ECO:0007829|PDB:4IQF"
FT STRAND 26..31
FT /evidence="ECO:0007829|PDB:4IQF"
FT TURN 39..42
FT /evidence="ECO:0007829|PDB:4IQF"
FT HELIX 48..55
FT /evidence="ECO:0007829|PDB:4IQF"
FT HELIX 69..78
FT /evidence="ECO:0007829|PDB:4IQF"
FT STRAND 81..87
FT /evidence="ECO:0007829|PDB:4IQF"
FT HELIX 94..97
FT /evidence="ECO:0007829|PDB:4IQF"
FT STRAND 104..110
FT /evidence="ECO:0007829|PDB:4IQF"
FT STRAND 114..118
FT /evidence="ECO:0007829|PDB:4IQF"
FT HELIX 120..126
FT /evidence="ECO:0007829|PDB:4IQF"
FT STRAND 130..138
FT /evidence="ECO:0007829|PDB:4IQF"
FT STRAND 148..155
FT /evidence="ECO:0007829|PDB:4IQF"
FT HELIX 162..185
FT /evidence="ECO:0007829|PDB:4IQF"
FT HELIX 196..198
FT /evidence="ECO:0007829|PDB:4IQF"
FT HELIX 207..210
FT /evidence="ECO:0007829|PDB:4IQF"
FT HELIX 218..226
FT /evidence="ECO:0007829|PDB:4IQF"
FT TURN 227..232
FT /evidence="ECO:0007829|PDB:4IQF"
FT STRAND 234..238
FT /evidence="ECO:0007829|PDB:4IQF"
FT STRAND 241..252
FT /evidence="ECO:0007829|PDB:4IQF"
FT STRAND 261..265
FT /evidence="ECO:0007829|PDB:4IQF"
FT STRAND 270..272
FT /evidence="ECO:0007829|PDB:4IQF"
FT STRAND 275..287
FT /evidence="ECO:0007829|PDB:4IQF"
FT HELIX 295..301
FT /evidence="ECO:0007829|PDB:4IQF"
SQ SEQUENCE 314 AA; 34736 MW; 8E81D02492DABEE2 CRC64;
MIKVVFMGTP DFSVPVLRRL IEDGYDVIGV VTQPDRPVGR KKVLTPTPVK VEAEKHGIPV
LQPLRIREKD EYEKVLALEP DLIVTAAFGQ IVPNEILEAP KYGCINVHAS LLPELRGGAP
IHYAIMEGKE KTGITIMYMV EKLDAGDILT QVEVEIEERE TTGSLFDKLS EAGAHLLSKT
VPLLIQGKLE PIKQNEEEVT FAYNIKREQE KIDWTKTGEE VYNHIRGLNP WPVAYTTLAG
QVVKVWWGEK VPVTKSAEAG TIVAIEEDGF VVATGNETGV KITELQPSGK KRMSCSQFLR
GTKPEIGTKL GENA
