ID   ALAE_ALIF1              Reviewed;         150 AA.
AC   Q5E684;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=L-alanine exporter AlaE {ECO:0000255|HAMAP-Rule:MF_00914};
GN   Name=alaE {ECO:0000255|HAMAP-Rule:MF_00914}; OrderedLocusNames=VF_0967;
OS   Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=312309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700601 / ES114;
RX   PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA   Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA   Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA   Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT   "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with
RT   pathogenic congeners.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
CC   -!- FUNCTION: Exports L-alanine. {ECO:0000255|HAMAP-Rule:MF_00914}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00914}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00914}.
CC   -!- SIMILARITY: Belongs to the AlaE exporter family. {ECO:0000255|HAMAP-
CC       Rule:MF_00914}.
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DR   EMBL; CP000020; AAW85462.1; -; Genomic_DNA.
DR   RefSeq; WP_005418661.1; NC_006840.2.
DR   RefSeq; YP_204350.1; NC_006840.2.
DR   AlphaFoldDB; Q5E684; -.
DR   STRING; 312309.VF_0967; -.
DR   EnsemblBacteria; AAW85462; AAW85462; VF_0967.
DR   GeneID; 64240922; -.
DR   KEGG; vfi:VF_0967; -.
DR   PATRIC; fig|312309.11.peg.964; -.
DR   eggNOG; ENOG502ZRFS; Bacteria.
DR   HOGENOM; CLU_126493_0_0_6; -.
DR   OMA; ADWHQIA; -.
DR   OrthoDB; 1555708at2; -.
DR   Proteomes; UP000000537; Chromosome I.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0034639; F:L-amino acid efflux transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032973; P:amino acid export across plasma membrane; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00914; L_Ala_exporter; 1.
DR   InterPro; IPR010574; Ala_export_AlaE.
DR   Pfam; PF06610; AlaE; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..150
FT                   /note="L-alanine exporter AlaE"
FT                   /id="PRO_0000415632"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        48..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
SQ   SEQUENCE   150 AA;  16513 MW;  6D4C4E32620092BA CRC64;
     MSKKGPFNIR NAAADTFAMV VFCFITGMFI EILISGMTFE QSLASRTLSI PVNIAIAWPY
     GVFRDFMLRQ GARISPSKFM KNIADLVAYV TFQSPAYAMI LLVVGATPEQ IITAVSSNVV
     VSCVMGVFYG YFLDACRKAF KVPGYYTPQA