ID   ALAE_CROS8              Reviewed;         158 AA.
AC   A7MI12;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=L-alanine exporter AlaE {ECO:0000255|HAMAP-Rule:MF_00914};
GN   Name=alaE {ECO:0000255|HAMAP-Rule:MF_00914}; OrderedLocusNames=ESA_00597;
OS   Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Cronobacter.
OX   NCBI_TaxID=290339;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-894;
RX   PubMed=20221447; DOI=10.1371/journal.pone.0009556;
RA   Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L.,
RA   Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A.,
RA   Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K.,
RA   McClelland M., Forsythe S.J.;
RT   "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic
RT   hybridization analysis with other Cronobacter species.";
RL   PLoS ONE 5:E9556-E9556(2010).
CC   -!- FUNCTION: Exports L-alanine. {ECO:0000255|HAMAP-Rule:MF_00914}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00914}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00914}.
CC   -!- SIMILARITY: Belongs to the AlaE exporter family. {ECO:0000255|HAMAP-
CC       Rule:MF_00914}.
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DR   EMBL; CP000783; ABU75881.1; -; Genomic_DNA.
DR   AlphaFoldDB; A7MI12; -.
DR   EnsemblBacteria; ABU75881; ABU75881; ESA_00597.
DR   KEGG; esa:ESA_00597; -.
DR   HOGENOM; CLU_126493_0_0_6; -.
DR   OMA; ADWHQIA; -.
DR   Proteomes; UP000000260; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0034639; F:L-amino acid efflux transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032973; P:amino acid export across plasma membrane; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00914; L_Ala_exporter; 1.
DR   InterPro; IPR010574; Ala_export_AlaE.
DR   Pfam; PF06610; AlaE; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..158
FT                   /note="L-alanine exporter AlaE"
FT                   /id="PRO_0000415620"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        92..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        117..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
SQ   SEQUENCE   158 AA;  17357 MW;  165C2BBD6F4355E7 CRC64;
     MSQELNSMFS PHSRLRHAVA DTFAMVVYCS VVGMMIEIFV SGMSFEQSLS SRLVAIPVNM
     VIAWPYGLYR DAVMRLAARV GKGRLVRNLA DVIAYITFQS PVYAAILLFV GADIPQIITA
     VSSNIVVSMM MGAAYGYFLD YCRRLFRVSP AAPVSAQA