FNBA_STAAW
ID FNBA_STAAW Reviewed; 1015 AA.
AC Q8NUU7;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=Fibronectin-binding protein A;
DE Flags: Precursor;
GN Name=fnbA; OrderedLocusNames=MW2421;
OS Staphylococcus aureus (strain MW2).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=196620;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MW2;
RX PubMed=12044378; DOI=10.1016/s0140-6736(02)08713-5;
RA Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y.,
RA Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K.;
RT "Genome and virulence determinants of high virulence community-acquired
RT MRSA.";
RL Lancet 359:1819-1827(2002).
CC -!- FUNCTION: Promotes bacterial attachment to multiple substrates, such as
CC fibronectin (Fn), fibrinogen (Fg), elastin peptides and tropoelastin.
CC This confers to S.aureus the ability to invade endothelial cells.
CC Promotes adherence to and aggregation of activated platelets (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00477}. Note=Anchored to the cell wall by sortase A (By
CC similarity). {ECO:0000250|UniProtKB:P14738}.
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DR EMBL; BA000033; BAB96286.1; -; Genomic_DNA.
DR RefSeq; WP_000794648.1; NC_003923.1.
DR AlphaFoldDB; Q8NUU7; -.
DR SMR; Q8NUU7; -.
DR EnsemblBacteria; BAB96286; BAB96286; BAB96286.
DR KEGG; sam:MW2421; -.
DR HOGENOM; CLU_009849_1_0_9; -.
DR OMA; DANKPGN; -.
DR PRO; PR:Q8NUU7; -.
DR Proteomes; UP000000418; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.1280; -; 1.
DR InterPro; IPR011266; Adhesin_Fg-bd_dom_2.
DR InterPro; IPR008966; Adhesion_dom_sf.
DR InterPro; IPR011252; Fibrogen-bd_dom1.
DR InterPro; IPR004237; Fibron_repeat-bd.
DR InterPro; IPR019931; LPXTG_anchor.
DR InterPro; IPR041171; SDR_Ig.
DR InterPro; IPR005877; YSIRK_signal_dom.
DR Pfam; PF17961; Big_8; 1.
DR Pfam; PF02986; Fn_bind; 3.
DR Pfam; PF00746; Gram_pos_anchor; 1.
DR Pfam; PF10425; SdrG_C_C; 1.
DR Pfam; PF04650; YSIRK_signal; 1.
DR SUPFAM; SSF49401; SSF49401; 2.
DR TIGRFAMs; TIGR01168; YSIRK_signal; 1.
DR PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE 3: Inferred from homology;
KW Cell adhesion; Cell wall; Peptidoglycan-anchor; Repeat; Secreted; Signal;
KW Virulence.
FT SIGNAL 1..36
FT /evidence="ECO:0000255"
FT CHAIN 37..982
FT /note="Fibronectin-binding protein A"
FT /id="PRO_0000313888"
FT PROPEP 983..1015
FT /note="Removed by sortase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT /id="PRO_0000313889"
FT REPEAT 546..575
FT /note="B-1"
FT REPEAT 576..605
FT /note="B-2"
FT REPEAT 746..783
FT /note="D-1"
FT REPEAT 784..821
FT /note="D-2"
FT REPEAT 822..860
FT /note="D-3"
FT REPEAT 861..875
FT /note="D-4; truncated"
FT REPEAT 876..889
FT /note="WR 1"
FT REPEAT 890..903
FT /note="WR 2"
FT REPEAT 904..917
FT /note="WR 3"
FT REPEAT 918..931
FT /note="WR 4"
FT REPEAT 932..945
FT /note="WR 5"
FT REGION 37..512
FT /note="Ligand-binding A region"
FT REGION 75..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 194..512
FT /note="Fibrinogen/elastin/tropoelastin-binding"
FT /evidence="ECO:0000250"
FT REGION 513..873
FT /note="Fibronectin-binding"
FT /evidence="ECO:0000250"
FT REGION 546..605
FT /note="2 X approximate tandem repeats"
FT REGION 596..623
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 741..815
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 746..875
FT /note="4 X approximate tandem repeats"
FT REGION 828..953
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 876..945
FT /note="5 X tandem repeats, Pro-rich (WR)"
FT REGION 966..992
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 7..18
FT /note="YSIRK-G/S signaling motif"
FT /evidence="ECO:0000250|UniProtKB:P14738"
FT MOTIF 979..983
FT /note="LPXTG sorting signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT COMPBIAS 75..110
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 113..127
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 128..173
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..195
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 792..806
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 859..873
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 874..933
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 982
FT /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
SQ SEQUENCE 1015 AA; 111146 MW; D0F9281BB64D44D2 CRC64;
MKNNLRYGIR KHKLGAASVF LGTMIVVGMG QDKEAAASEQ KTTTVEENGN SATENKVNET
QTTTTNVNTI DETQSYSATA TEQPSNATQV TTEEAPKAVQ APQTAQPANL ETVKEEVVKE
EAKPQVKETT QSQDNSGDQR QVDLTPKKAT QNQVAETQVE VAQPRTASES KPRVTRSADV
VEAKEASDEK VETGTDVTSK VTVESGSIEA PQGNKVEPHA GQRVVLKYKL KFADGLKRGD
YFDFTLSNNV NTYGVSTARK VPEIKNGSVV MATGEILGNG NIRYTFTNEI EHKVEVTANL
EINLFIDPKT VQSNGEQKIT SKLNGEETEK TIPVVYNPGV SNSYTNVNGS IETFNKESNK
FTHIAYIKPM NGNQSNTVSV TGTLTEGSNL AGGQPTVKVY EYLGKKDELP QSVYANTSDT
NKFKDVTKEM NGKLSVQDNG SYSLNLDKLD KTYVIHYTGE YLQGSDQVNF RTELYGYPER
AYKSYYVYGG YRLTWDNGLV LYSNKADGNG KNGQIIQNND FEYKEDTAKG TMSGQYDAKQ
IIETEENQDN TPLDIDYHTA IDGEGGYVDG YIETIEETDS SAIDIDYHTA VDSEAGHVGG
YTESSEESNP IDFEESTHEN SKHHADVVEY EEDTNPGGGQ VTTESNLVEF DEESTKGIVT
GAVSDHTTIE DTKEYTTESN LIELVDELPE EHGQAQGPIE EITENNHHIS HSGLGTENGH
GNYGVIEEIE ENSHVDIKSE LGYEGGQNSG NQSFEEDTEE DKPKYEQGGN IVDIDFDSVP
QIQGQNNGNQ SFEEDTEKDK PKYEQGGNII DIDFDSVPQI HGFNKHTEII EEDTNKDKPN
YQFGGHNSVD FEEDTLPKVS GQNEGQQTIE EDTTPPTPPT PEVPSEPETP TPPTPEVPSE
PETPTPPTPE VPSEPETPTP PTPEVPAEPG KPVPPAKEEP KKPSKPVEQG KVVTPVIEIN
EKVKAVAPTK KAQSKKSELP ETGGEESTNK GMLFGGLFSI LGLALLRRNK KNNKA
