ID   ALAE_ECO57              Reviewed;         149 AA.
AC   P64552; P76626;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=L-alanine exporter AlaE {ECO:0000255|HAMAP-Rule:MF_00914};
GN   Name=alaE {ECO:0000255|HAMAP-Rule:MF_00914};
GN   OrderedLocusNames=Z3970, ECs3531;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Exports L-alanine. {ECO:0000255|HAMAP-Rule:MF_00914}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00914}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00914}.
CC   -!- SIMILARITY: Belongs to the AlaE exporter family. {ECO:0000255|HAMAP-
CC       Rule:MF_00914}.
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DR   EMBL; AE005174; AAG57778.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB36954.1; -; Genomic_DNA.
DR   PIR; C91070; C91070.
DR   PIR; F85914; F85914.
DR   RefSeq; NP_311558.1; NC_002695.1.
DR   RefSeq; WP_000492656.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P64552; -.
DR   STRING; 155864.EDL933_3834; -.
DR   EnsemblBacteria; AAG57778; AAG57778; Z3970.
DR   EnsemblBacteria; BAB36954; BAB36954; ECs_3531.
DR   GeneID; 66673460; -.
DR   GeneID; 914753; -.
DR   KEGG; ece:Z3970; -.
DR   KEGG; ecs:ECs_3531; -.
DR   PATRIC; fig|386585.9.peg.3685; -.
DR   eggNOG; ENOG502ZRFS; Bacteria.
DR   HOGENOM; CLU_126493_0_0_6; -.
DR   OMA; ADWHQIA; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0034639; F:L-amino acid efflux transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032973; P:amino acid export across plasma membrane; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00914; L_Ala_exporter; 1.
DR   InterPro; IPR010574; Ala_export_AlaE.
DR   Pfam; PF06610; AlaE; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..149
FT                   /note="L-alanine exporter AlaE"
FT                   /id="PRO_0000169303"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        112..132
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
SQ   SEQUENCE   149 AA;  16949 MW;  0B1D31F498DBE4BC CRC64;
     MFSPQSRLRH AVADTFAMVV YCSVVNMCIE VFLSGMSFEQ SFYSRLVAIP VNILIAWPYG
     MYRDLFMRAA RKVSPSGWIK NLADILAYVT FQSPVYVAIL LVVGADWHQI MAAVSSNIVV
     SMLMGAVYGY FLDYCRRLFK VSRYQQVKA