ALAE_ECOLI
ID ALAE_ECOLI Reviewed; 149 AA.
AC P64550; P76626; Q2MAC5;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=L-alanine exporter AlaE {ECO:0000255|HAMAP-Rule:MF_00914};
GN Name=alaE {ECO:0000255|HAMAP-Rule:MF_00914}; Synonyms=ygaW;
GN OrderedLocusNames=b2670, JW2645;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP FUNCTION IN ALANINE EXPORT, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION
RP PHENOTYPE, AND GENE NAME.
RC STRAIN=K12;
RX PubMed=21531828; DOI=10.1128/aem.00003-11;
RA Hori H., Yoneyama H., Tobe R., Ando T., Isogai E., Katsumata R.;
RT "Inducible L-alanine exporter encoded by the novel gene ygaW (alaE) in
RT Escherichia coli.";
RL Appl. Environ. Microbiol. 77:4027-4034(2011).
CC -!- FUNCTION: Exports L-alanine. {ECO:0000255|HAMAP-Rule:MF_00914,
CC ECO:0000269|PubMed:21531828}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_00914, ECO:0000269|PubMed:21531828}; Multi-pass membrane
CC protein {ECO:0000255|HAMAP-Rule:MF_00914, ECO:0000269|PubMed:21531828}.
CC -!- INDUCTION: Induced in the presence of L-alanyl-L-alanine dipeptides.
CC {ECO:0000269|PubMed:21531828}.
CC -!- DISRUPTION PHENOTYPE: Mutants show a significant accumulation of
CC intracellular L-alanine and a reduction in the L-alanine export rate.
CC {ECO:0000269|PubMed:21531828}.
CC -!- SIMILARITY: Belongs to the AlaE exporter family. {ECO:0000255|HAMAP-
CC Rule:MF_00914}.
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DR EMBL; U00096; AAC75717.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76781.1; -; Genomic_DNA.
DR PIR; G65046; G65046.
DR RefSeq; NP_417156.1; NC_000913.3.
DR RefSeq; WP_000492656.1; NZ_STEB01000042.1.
DR AlphaFoldDB; P64550; -.
DR BioGRID; 4261134; 9.
DR STRING; 511145.b2670; -.
DR PaxDb; P64550; -.
DR PRIDE; P64550; -.
DR EnsemblBacteria; AAC75717; AAC75717; b2670.
DR EnsemblBacteria; BAE76781; BAE76781; BAE76781.
DR GeneID; 66673460; -.
DR GeneID; 947147; -.
DR KEGG; ecj:JW2645; -.
DR KEGG; eco:b2670; -.
DR PATRIC; fig|1411691.4.peg.4071; -.
DR EchoBASE; EB3297; -.
DR eggNOG; ENOG502ZRFS; Bacteria.
DR HOGENOM; CLU_126493_0_0_6; -.
DR OMA; ADWHQIA; -.
DR PhylomeDB; P64550; -.
DR BioCyc; EcoCyc:G7399-MON; -.
DR BioCyc; MetaCyc:G7399-MON; -.
DR PRO; PR:P64550; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0034639; F:L-amino acid efflux transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015299; F:solute:proton antiporter activity; IDA:EcoCyc.
DR GO; GO:0032973; P:amino acid export across plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0015808; P:L-alanine transport; IDA:EcoCyc.
DR HAMAP; MF_00914; L_Ala_exporter; 1.
DR InterPro; IPR010574; Ala_export_AlaE.
DR Pfam; PF06610; AlaE; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..149
FT /note="L-alanine exporter AlaE"
FT /id="PRO_0000169302"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
SQ SEQUENCE 149 AA; 16949 MW; 0B1D31F498DBE4BC CRC64;
MFSPQSRLRH AVADTFAMVV YCSVVNMCIE VFLSGMSFEQ SFYSRLVAIP VNILIAWPYG
MYRDLFMRAA RKVSPSGWIK NLADILAYVT FQSPVYVAIL LVVGADWHQI MAAVSSNIVV
SMLMGAVYGY FLDYCRRLFK VSRYQQVKA
