FNDC1_RAT
ID FNDC1_RAT Reviewed; 1779 AA.
AC Q2Q0I9;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 2.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Fibronectin type III domain-containing protein 1;
DE AltName: Full=Activator of G-protein signaling 8;
DE Flags: Precursor;
GN Name=Fndc1; Synonyms=Ags8;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 50-1779, FUNCTION, AND INDUCTION BY ISCHEMIA.
RC STRAIN=Wistar; TISSUE=Heart;
RX PubMed=16407149; DOI=10.1073/pnas.0507467103;
RA Sato M., Cismowski M.J., Toyota E., Smrcka A.V., Lucchesi P.A.,
RA Chilian W.M., Lanier S.M.;
RT "Identification of a receptor-independent activator of G protein signaling
RT (AGS8) in ischemic heart and its interaction with Gbetagamma.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:797-802(2006).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=16641100; DOI=10.1073/pnas.0600895103;
RA Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.;
RT "Quantitative phosphoproteomics of vasopressin-sensitive renal cells:
RT regulation of aquaporin-2 phosphorylation at two sites.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-699, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: May be an activator of G protein signaling.
CC {ECO:0000269|PubMed:16407149}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- INDUCTION: By ischemia. {ECO:0000269|PubMed:16407149}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-50 is the initiator.
CC {ECO:0000305}.
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DR EMBL; AABR03000391; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03002558; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03004065; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DQ256268; ABB82299.1; -; mRNA.
DR RefSeq; NP_001033704.1; NM_001038615.1.
DR AlphaFoldDB; Q2Q0I9; -.
DR SMR; Q2Q0I9; -.
DR DIP; DIP-61094N; -.
DR IntAct; Q2Q0I9; 2.
DR STRING; 10116.ENSRNOP00000029878; -.
DR GlyGen; Q2Q0I9; 1 site.
DR iPTMnet; Q2Q0I9; -.
DR PhosphoSitePlus; Q2Q0I9; -.
DR PaxDb; Q2Q0I9; -.
DR PRIDE; Q2Q0I9; -.
DR Ensembl; ENSRNOT00000034842; ENSRNOP00000029878; ENSRNOG00000030210.
DR GeneID; 308099; -.
DR KEGG; rno:308099; -.
DR CTD; 84624; -.
DR RGD; 1310640; Fndc1.
DR eggNOG; KOG4221; Eukaryota.
DR GeneTree; ENSGT00530000063558; -.
DR InParanoid; Q2Q0I9; -.
DR PhylomeDB; Q2Q0I9; -.
DR TreeFam; TF337588; -.
DR PRO; PR:Q2Q0I9; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000030210; Expressed in esophagus and 18 other tissues.
DR ExpressionAtlas; Q2Q0I9; baseline and differential.
DR Genevisible; Q2Q0I9; RN.
DR GO; GO:0005911; C:cell-cell junction; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0031966; C:mitochondrial membrane; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0071456; P:cellular response to hypoxia; IMP:MGI.
DR GO; GO:0010666; P:positive regulation of cardiac muscle cell apoptotic process; IMP:MGI.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; IDA:MGI.
DR GO; GO:0051223; P:regulation of protein transport; IDA:MGI.
DR CDD; cd00063; FN3; 4.
DR Gene3D; 2.60.40.10; -; 5.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR Pfam; PF00041; fn3; 3.
DR SMART; SM00060; FN3; 3.
DR SUPFAM; SSF49265; SSF49265; 4.
DR PROSITE; PS50853; FN3; 4.
PE 1: Evidence at protein level;
KW Glycoprotein; Phosphoprotein; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..1779
FT /note="Fibronectin type III domain-containing protein 1"
FT /id="PRO_0000284832"
FT DOMAIN 33..126
FT /note="Fibronectin type-III 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 103..203
FT /note="Fibronectin type-III 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 207..302
FT /note="Fibronectin type-III 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 307..402
FT /note="Fibronectin type-III 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 1543..1637
FT /note="Fibronectin type-III 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT REGION 400..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 459..1108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1120..1227
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1330..1401
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 400..434
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 495..510
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 534..549
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 569..583
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 586..611
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 625..668
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 679..695
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 762..786
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 816..830
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 863..932
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 952..981
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 999..1019
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1047..1061
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1072..1086
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1137..1181
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1330..1390
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 651
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:16641100"
FT MOD_RES 699
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT CARBOHYD 1546
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 439
FT /note="M -> T (in Ref. 2; ABB82299)"
FT /evidence="ECO:0000305"
FT CONFLICT 441
FT /note="P -> S (in Ref. 2; ABB82299)"
FT /evidence="ECO:0000305"
FT CONFLICT 493
FT /note="N -> D (in Ref. 2; ABB82299)"
FT /evidence="ECO:0000305"
FT CONFLICT 1016
FT /note="P -> R (in Ref. 2; ABB82299)"
FT /evidence="ECO:0000305"
FT CONFLICT 1423
FT /note="P -> L (in Ref. 2; ABB82299)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1779 AA; 194049 MW; B9F21FBEAF3233A4 CRC64;
MAPEARASPR LLLRAALLLL AALLPVASSA GPPVDHPLKP RHVKLLSANM GLKVTWDPPK
DATSRPVEHY NIAYGKSLKS LKSIKVNAET HSFLIKDVEK EVPNKPLRMR VRASDDRLSV
AWKAPRLSGA KSPRRSRGFL LGYGESGRKM NYVPLTRDER SHEIKKLASE SVYVVSLQST
NSQGQSQPVY RAALTKRKNA EEDELDVPED ISVRVMSSQS VLVAWVDPLV EKQKRVVASR
QYTVRYREKG ESARWDYKQV SNRRALVDSL IPDTVYEFAV RISQGERDGK WSASVFQRTP
ESAPTTAPEN LRVWPVNGKP TVVTVSWDAL PESEGKVKEY ILSYAPALKP FGAKSLTFSG
HTTSALVDGL QPGERYLFKI RATNRRGQGP HSKAFIVAIP TTSSTEASVQ PNGRDNGKPE
KPQQPSSSAP KVAASSQHMP PAKNVKDALS DLKNKIQTNG VAPGRTQLHS KVGELDPQST
EVTGEEELDS LENPRSSRLE TLNQKQPLRV PSRSGHGALA PGRTPARAGL PVLSRKEGMD
RRGPSLDPHP HPRVEPSASS AYHQLSSTDN DSVDRKEDDQ AGSPDPKAAS SGSSPKNPGR
SRPTSAPSRH AASNMLRDKS RVHPGTKAAS SSTSRQSHSS TSEEDSSAQP SRHFPLHRGS
STSPLSRGWK DRQDTHASSS HTTSRTASSS HPSALTEGSE EEDGGADSDR AAEDTIRRAE
ATAQIQQTRP GLGHFSLIRN KPFTPHSRNP NRFPRLRGPR LQPSVSPQST SASKVLTRSP
SLPASHTRPG SDVYGDGEDE EPLPATVIND RTPSYPRHPI SGSSDTLRRG PQRGASLYRK
EPIPENSKAA GADVPPGGRS PLSSKAQGFQ QSTTDEGAPQ TSPASTSRQP SPARPPASRS
QPSPGSTVPR RMTPDRSSEL SSSQSKDRSL SQPKLSVAHA GHDHPHTANS RGVLPSAPQN
QNEGAQSTYE DNSTEIEGPD SRTPTHSARA KDTTPPILKP RQVGSQSWSS DNRPQPSQAG
ASERPIRPGS THPRAQVPGR AGVQATSVKK VSPSKRPLPL ESQQSVFAEE EEENEGMLKG
KEDSLSTSVK KWPSSSSPRD KYADRNLDKD KAAIGLLVQE ENTVPGRRPP GSPAIASHPS
TRHQPRNPAT ASPIANTHSW PRYTTRAPSS YSSTTPMLSL RQRMQRRFRT PVSRQPPPPR
PVLTPGYNGR PNAEENIPPG SIGKPNGQRI INGPQGTKWV VDLDRGLVLN AEGRYLQDSH
GNPLRVRLGG DGRTIVDLGG TPMVSPDGLP LFGQGRHGKP VASAQDKPIL SLGGKPLVGL
EVVRTTTQVP TTTMPPSTTT TTVPPTTTLP PTTTTTRRTT TTRRTTTTRR PTTTTRATRR
TTTTTTTPEP TTPSPTCPPG TLEHRDEAGN LIMGSNGIPE CYPEEDDFSG LEIDTALPTE
EDYVVYDDDY GLETTRPPTS TMPSTTAATP KVVPEQGTVS SFPEEEFDLA GKRRFVAPYV
TYLSKDPAAP CSLTDALDHF QVESLDELIP NDLTKNDLPP QHAPRNITVV AMEGCHSFVI
VDWNKAIPGD VVTGYLVYSA SYEDFIRNKW STQTSSVTHL PIENLKPNTR YYFKVQAKNP
HGYGPVSPSV SFVTESDNPL LVVRPPGGEP IWIPFAFKHD PGYTDCHGRQ YVKRTWYKKF
VGVVLCNSLR YKIYLSDNLK DTFYSIGDSW GRGEDHCQFV DSHLDGRTGP QSYVEALPTI
QGYYRQYRQE PVSFGHIGFG TPYYYVGWYE CGVSIPGKW
