ID   FNR_AGGAC               Reviewed;         257 AA.
AC   Q9EXQ1; Q46157;
DT   30-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Anaerobic regulatory protein;
GN   Name=fnr; Synonyms=actX;
OS   Aggregatibacter actinomycetemcomitans (Actinobacillus
OS   actinomycetemcomitans) (Haemophilus actinomycetemcomitans).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Aggregatibacter.
OX   NCBI_TaxID=714;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 43718 / FDC Y4 / Serotype b;
RX   PubMed=11130862; DOI=10.1016/s0923-2508(00)01137-2;
RA   Kokeguchi S., Hirosue M., Maeda H., Miyamoto M., Takashiba S., Murayama Y.;
RT   "Molecular characterization of the hlyX-like gene of Actinobacillus
RT   actinomycetemcomitans Y4.";
RL   Res. Microbiol. 151:721-725(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 125-216.
RC   STRAIN=301-b / Serotype a;
RX   PubMed=8998988; DOI=10.1111/j.1574-6968.1996.tb08108.x;
RA   Hattori T., Takahashi K., Nakanishi T., Ohta H., Fukui K., Taniguchi S.,
RA   Takigawa M.;
RT   "Novel FNR homologues identified in four representative oral facultative
RT   anaerobes: Capnocytophaga ochracea, Capnocytophaga sputigena, Haemophilus
RT   aphrophilus, and Actinobacillus actinomycetemcomitans.";
RL   FEMS Microbiol. Lett. 137:213-220(1996).
CC   -!- FUNCTION: It is involved in the activation of genes necessary for
CC       anaerobic respiration. It probably also activates genes involved in the
CC       production of virulence factors.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit. {ECO:0000250};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- INDUCTION: By anaerobiosis.
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DR   EMBL; D89096; BAB19627.1; -; Genomic_DNA.
DR   EMBL; D78251; BAA11315.1; -; Genomic_DNA.
DR   RefSeq; WP_005568756.1; NZ_CP065604.1.
DR   AlphaFoldDB; Q9EXQ1; -.
DR   SMR; Q9EXQ1; -.
DR   STRING; 714.ACT75_00055; -.
DR   eggNOG; COG0664; Bacteria.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd00038; CAP_ED; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR018490; cNMP-bd-like.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR012318; HTH_CRP.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR018335; Tscrpt_reg_HTH_Crp-type_CS.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00027; cNMP_binding; 1.
DR   Pfam; PF13545; HTH_Crp_2; 1.
DR   PRINTS; PR00034; HTHCRP.
DR   SMART; SM00100; cNMP; 1.
DR   SMART; SM00419; HTH_CRP; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF51206; SSF51206; 1.
DR   PROSITE; PS50042; CNMP_BINDING_3; 1.
DR   PROSITE; PS00042; HTH_CRP_1; 1.
DR   PROSITE; PS51063; HTH_CRP_2; 1.
PE   2: Evidence at transcript level;
KW   4Fe-4S; Activator; DNA-binding; Iron; Iron-sulfur; Metal-binding;
KW   Transcription; Transcription regulation; Virulence.
FT   CHAIN           1..257
FT                   /note="Anaerobic regulatory protein"
FT                   /id="PRO_0000100172"
FT   DOMAIN          167..240
FT                   /note="HTH crp-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT   DNA_BIND        200..219
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT   REGION          23..32
FT                   /note="Essential for the oxygen-regulated activity"
FT                   /evidence="ECO:0000250"
FT   BINDING         23
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
FT   BINDING         26
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
FT   BINDING         32
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
FT   BINDING         125
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        189
FT                   /note="F -> S (in Ref. 2; BAA11315)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   257 AA;  28929 MW;  A4D82081369BD042 CRC64;
     MKILATETKL GRRVQSGGCA IHCQNCSISQ LCIPFTLNQH ELDQLDNIIE RKKPIQKSQV
     LFKAGDELTS LYAIRSGTIK SYTISETGEE QITSFHLPGD LVGFDAIMNM QHPSFAQALE
     TAMVCEIPFD ILDDLSGKMP KLRQQIMRLM SNEIKSDQEM ILLLSKMNAE ERLAAFIYNL
     SQRYSARGFS AREFRLTMTR GDIGNYLGLT VETISRLLGR FQKLGVLSVQ GKYITINNMA
     ELIELSGTNK NKIQLII