FOCAD_MOUSE
ID FOCAD_MOUSE Reviewed; 1798 AA.
AC A2AKG8; Q0P620; Q3TPY9; Q3UJY8; Q3UK73; Q6PGE3;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Focadhesin;
GN Name=Focad; Synonyms=Kiaa1797;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1183-1798 (ISOFORM 1).
RC STRAIN=C57BL/6J, and DBA/2J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 483-1798 (ISOFORM 1).
RC STRAIN=C57BL/6J, and FVB/N-3; TISSUE=Embryonic brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=22427331; DOI=10.1093/brain/aws045;
RA Brockschmidt A., Trost D., Peterziel H., Zimmermann K., Ehrler M.,
RA Grassmann H., Pfenning P.N., Waha A., Wohlleber D., Brockschmidt F.F.,
RA Jugold M., Hoischen A., Kalla C., Waha A., Seifert G., Knolle P.A.,
RA Latz E., Hans V.H., Wick W., Pfeifer A., Angel P., Weber R.G.;
RT "KIAA1797/FOCAD encodes a novel focal adhesion protein with tumour
RT suppressor function in gliomas.";
RL Brain 135:1027-1041(2012).
CC -!- FUNCTION: Potential tumor suppressor in gliomas.
CC {ECO:0000269|PubMed:22427331}.
CC -!- SUBUNIT: Interacts with VCL. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}. Cell junction, focal adhesion. Note=Colocalizes
CC with VCL in astrocytes. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=A2AKG8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2AKG8-2; Sequence=VSP_030275, VSP_030276;
CC Name=3;
CC IsoId=A2AKG8-3; Sequence=VSP_030274;
CC -!- TISSUE SPECIFICITY: Expressed by glial and neuronal cells in brain.
CC {ECO:0000269|PubMed:22427331}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE26929.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAE27017.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK146138; BAE26929.1; ALT_INIT; mRNA.
DR EMBL; AK146256; BAE27017.1; ALT_INIT; mRNA.
DR EMBL; AK164030; BAE37596.1; -; mRNA.
DR EMBL; AL772296; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL772316; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL831756; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX572638; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC039622; AAH39622.1; -; mRNA.
DR EMBL; BC057079; AAH57079.1; -; mRNA.
DR CCDS; CCDS38798.1; -. [A2AKG8-1]
DR RefSeq; NP_001074653.1; NM_001081184.1. [A2AKG8-1]
DR AlphaFoldDB; A2AKG8; -.
DR BioGRID; 230958; 1.
DR STRING; 10090.ENSMUSP00000095602; -.
DR iPTMnet; A2AKG8; -.
DR PhosphoSitePlus; A2AKG8; -.
DR EPD; A2AKG8; -.
DR MaxQB; A2AKG8; -.
DR PaxDb; A2AKG8; -.
DR PeptideAtlas; A2AKG8; -.
DR PRIDE; A2AKG8; -.
DR ProteomicsDB; 271707; -. [A2AKG8-1]
DR ProteomicsDB; 271708; -. [A2AKG8-2]
DR ProteomicsDB; 271709; -. [A2AKG8-3]
DR Antibodypedia; 66171; 10 antibodies from 6 providers.
DR Ensembl; ENSMUST00000097992; ENSMUSP00000095602; ENSMUSG00000038368. [A2AKG8-1]
DR Ensembl; ENSMUST00000107147; ENSMUSP00000102765; ENSMUSG00000038368. [A2AKG8-3]
DR GeneID; 230393; -.
DR KEGG; mmu:230393; -.
DR UCSC; uc008tmt.1; mouse. [A2AKG8-2]
DR UCSC; uc008tmu.1; mouse. [A2AKG8-1]
DR CTD; 54914; -.
DR MGI; MGI:2676921; Focad.
DR VEuPathDB; HostDB:ENSMUSG00000038368; -.
DR eggNOG; ENOG502QQKG; Eukaryota.
DR GeneTree; ENSGT00390000004438; -.
DR HOGENOM; CLU_1342874_0_0_1; -.
DR InParanoid; A2AKG8; -.
DR OMA; EWISMVL; -.
DR OrthoDB; 187102at2759; -.
DR PhylomeDB; A2AKG8; -.
DR TreeFam; TF323261; -.
DR BioGRID-ORCS; 230393; 1 hit in 74 CRISPR screens.
DR ChiTaRS; Focad; mouse.
DR PRO; PR:A2AKG8; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; A2AKG8; protein.
DR Bgee; ENSMUSG00000038368; Expressed in animal zygote and 224 other tissues.
DR ExpressionAtlas; A2AKG8; baseline and differential.
DR Genevisible; A2AKG8; MM.
DR GO; GO:0005925; C:focal adhesion; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0060147; P:regulation of post-transcriptional gene silencing; IEA:InterPro.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR022542; FOCAD/RST1_DUF3730.
DR InterPro; IPR021392; Focadhesin.
DR InterPro; IPR045163; Focadhesin/RST1.
DR PANTHER; PTHR16212; PTHR16212; 1.
DR Pfam; PF12530; DUF3730; 1.
DR Pfam; PF11229; Focadhesin; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cell junction; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..1798
FT /note="Focadhesin"
FT /id="PRO_0000314458"
FT TRANSMEM 441..461
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1033..1053
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 816
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q5VW36"
FT VAR_SEQ 1..1594
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_030274"
FT VAR_SEQ 332..335
FT /note="ALKL -> GASK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030275"
FT VAR_SEQ 336..1798
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030276"
FT CONFLICT 638
FT /note="Q -> R (in Ref. 3; AAH57079)"
FT /evidence="ECO:0000305"
FT CONFLICT 679
FT /note="V -> A (in Ref. 3; AAH57079)"
FT /evidence="ECO:0000305"
FT CONFLICT 1180
FT /note="F -> I (in Ref. 3; AAH57079)"
FT /evidence="ECO:0000305"
FT CONFLICT 1521
FT /note="H -> R (in Ref. 1; BAE26929/BAE27017)"
FT /evidence="ECO:0000305"
FT CONFLICT 1623
FT /note="Q -> K (in Ref. 1; BAE26929/BAE27017)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1798 AA; 198950 MW; 129DC68F61647C09 CRC64;
MSDDIKKRFD FPNSLIQSQA VGHLIAAVLK EHGVSEKIHQ STNQTAALNL LWEKCCSDHV
VVRTACSEGL VALVAQGHAE FSYVLNGILN LTPSARNMSG LIKALMKLLQ MQALREGQSG
EKDIQDIYSI RNPPQPLITV LEHRPDCWPL LLQQLTAFFQ QCPERSEASC VQIMAPFLWY
LYCEPSQLEE YAKLRLSLLK VLLQPRGVCE EAQLSVREQS TLQLCCSMLP CLQMKDLMQT
TEMMLFVEEV YLSLLRHPGF WKSQLTQLTL QLLCICEVSL KITGECSSLI HLLERSVELL
GEDFPVELVI IGIALLLLQM PASQQKPLLN LALKLLSLTE GQKIPKASLL LLVPLLQILS
STALEDCLAM GEEGPSRQQL ALSLVEVLQK ERHGDDSHTI SCRLAFPVSS MYGSIFTAWR
ILDVIGEESA TSDWLATVES LLSATTVIPP HVFLLLAYLL VEDVGQNLQQ ILRVTTQLAQ
ADSSQVPNLI PVLMFKLGRP LDPVSYNHIL YTLPTLGVHK VCVGQILRVI QLLGTTPRLR
AVTLRLLTSL WEKQDRVYPE LQRFMAVSDA PSLSVGKELQ WEKLIAKAAS IRDICKQRPY
QHGADMLAAI SQVLNECTKP DQATPAALVL QGLHALCQAE VVCIRSTWNA LSPKLSCDMR
PLILKTLSEL FSLVPSLTVN TVEYENFKVQ VLSFLWTHTQ NKNPTVASAA YKSLSHFSAG
EHTILHLPEK IRPEMPVPGE LDEEESVDLS IPGACFLRLL TITAPSVLPA LEEFFTSLVR
QEMVNMPRGI YHSALKGGVR SDQGKTVAGI PNFILKTYET NKQPGLKPGL AGGMLFCYDL
AMYQSKDGKP LNRLMASRGR SFKQTTLALI HEVHVQLSEW HRAIFLPQAW LAYMTRAYHA
ILQGRIAELE LQLQHGKEGP EEVQYKRSTA WLWVRDMLTD EITKTAAKES PVVKANALLA
LSSLAVVVSK HEASLCSDSD GVLEIQPNFL PVKEWVSMVL NTLLVIVDSH YHPSGQLFTC
FYHKSYSGEN TASAIARSAA ATALSLLVPV FIISCKEKVE EILNMLTARL PGKPSADESQ
AVQIHMGLAL GMFLSRLCEE KLSDMSGQQM NLLLMKSLDA LESCCFDPSL EYNTGCILGV
GLALSLMSHS SHTESRVHVA ASLRKLSTYL DESGSQSRTF QEVLVYTLSC VCTSAFSAGI
IEAAEAEDIM SKLQLLVENN QQTSGFALAL GNLVYGLSVC GHGKAEDLGN RLRPSWIKVV
LTEGAPTMLC LAALHGLVAL VGSDVDVMQL KSEAIQNTHF QARLNEVIRT LTEVISVSGV
IGLQSNAIWL LGHLHLSTLS SNQSRTSVPT DYSYLPEGSF IRAAIGFFIT GGKKGPEAVP
PSLLKVVMKP IATVGESYQY PPANLAALLS PLMRLNFGEE IQQLCLEIAV TQAPSSQSAA
SLLGLWVMPP LIHGLSLNIK KYLLVSMPLW AKHVSDEQIQ GFVENLMVEV FKTASQPCHP
EMCLSALQGL SQAMKLPSPS HHLWSLLCDA TGRIFDLLPN RIRRNDLELY ISIAKCLSEM
TDEGVNQVSQ ITKDNIEKAA FVKLYLVSQG RLPLMSLTDL LTAAMQHPEK ETLAWMILHS
LYQARIVNHA NTGVLKRLEW LLELMGYVRN IAYQSAPIQN VAPEEALDFL MLIFAAAVVA
WADHEAPLLL GLSASWLPWH QQNGPGGPAA ALLGRSPMHR VTVQEVLTQL PRSMLLLLQK
EPWKEQTQKF IDWLLSIMEI PNKAFAAKSK DLLKATLLSL RVLPEFKKKA VWTRAYGW
