FOCA_ECOLI
ID FOCA_ECOLI Reviewed; 285 AA.
AC P0AC23; P21501;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Probable formate transporter 1;
DE AltName: Full=Formate channel 1;
GN Name=focA; Synonyms=ycaE; OrderedLocusNames=b0904, JW0887;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2651404; DOI=10.1128/jb.171.5.2485-2498.1989;
RA Sawers G., Boeck A.;
RT "Novel transcriptional control of the pyruvate formate-lyase gene: upstream
RT regulatory sequences and multiple promoters regulate anaerobic
RT expression.";
RL J. Bacteriol. 171:2485-2498(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP CHARACTERIZATION.
RX PubMed=8022272; DOI=10.1111/j.1365-2958.1994.tb00375.x;
RA Suppmann B., Sawers G.;
RT "Isolation and characterization of hypophosphite-resistant mutants of
RT Escherichia coli: identification of the FocA protein, encoded by the pfl
RT operon, as a putative formate transporter.";
RL Mol. Microbiol. 11:965-982(1994).
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Involved in the bidirectional transport of formate.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the FNT transporter (TC 2.A.44) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA20390.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AP009048; BAA35639.1; -; Genomic_DNA.
DR EMBL; M26413; AAA20390.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC73990.1; -; Genomic_DNA.
DR PIR; A32305; A32305.
DR RefSeq; NP_415424.1; NC_000913.3.
DR RefSeq; WP_000642546.1; NZ_SSZK01000002.1.
DR AlphaFoldDB; P0AC23; -.
DR SMR; P0AC23; -.
DR BioGRID; 4260836; 8.
DR DIP; DIP-9670N; -.
DR IntAct; P0AC23; 1.
DR STRING; 511145.b0904; -.
DR TCDB; 1.A.16.1.1; the formate-nitrite transporter (fnt) family.
DR jPOST; P0AC23; -.
DR PaxDb; P0AC23; -.
DR PRIDE; P0AC23; -.
DR EnsemblBacteria; AAC73990; AAC73990; b0904.
DR EnsemblBacteria; BAA35639; BAA35639; BAA35639.
DR GeneID; 67414208; -.
DR GeneID; 945513; -.
DR KEGG; ecj:JW0887; -.
DR KEGG; eco:b0904; -.
DR PATRIC; fig|1411691.4.peg.1372; -.
DR EchoBASE; EB1238; -.
DR eggNOG; COG2116; Bacteria.
DR HOGENOM; CLU_036896_3_0_6; -.
DR InParanoid; P0AC23; -.
DR OMA; MIWFPIM; -.
DR PhylomeDB; P0AC23; -.
DR BioCyc; EcoCyc:FOCA-MON; -.
DR BioCyc; MetaCyc:FOCA-MON; -.
DR PRO; PR:P0AC23; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015499; F:formate transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR GO; GO:0015724; P:formate transport; IMP:EcoCyc.
DR GO; GO:0019664; P:mixed acid fermentation; IMP:EcoCyc.
DR GO; GO:0010447; P:response to acidic pH; TAS:EcoCyc.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR000292; For/NO2_transpt.
DR InterPro; IPR024002; For/NO2_transpt_CS.
DR InterPro; IPR023999; Formate_transptr_FocA_put.
DR PANTHER; PTHR30520; PTHR30520; 1.
DR Pfam; PF01226; Form_Nir_trans; 1.
DR TIGRFAMs; TIGR00790; fnt; 1.
DR TIGRFAMs; TIGR04060; formate_focA; 1.
DR PROSITE; PS01005; FORMATE_NITRITE_TP_1; 1.
DR PROSITE; PS01006; FORMATE_NITRITE_TP_2; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..285
FT /note="Probable formate transporter 1"
FT /id="PRO_0000094717"
FT TOPO_DOM 1..36
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 37..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..68
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 86..116
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 134..164
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..191
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..259
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..279
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 280..285
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 285 AA; 30991 MW; E0B06FFFC362DE16 CRC64;
MKADNPFDLL LPAAMAKVAE EAGVYKATKH PLKTFYLAIT AGVFISIAFV FYITATTGTG
TMPFGMAKLV GGICFSLGLI LCVVCGADLF TSTVLIVVAK ASGRITWGQL AKNWLNVYFG
NLVGALLFVL LMWLSGEYMT ANGQWGLNVL QTADHKVHHT FIEAVCLGIL ANLMVCLAVW
MSYSGRSLMD KAFIMVLPVA MFVASGFEHS IANMFMIPMG IVIRDFASPE FWTAVGSAPE
NFSHLTVMNF ITDNLIPVTI GNIIGGGLLV GLTYWVIYLR ENDHH
