FOCB_ECOLI
ID FOCB_ECOLI Reviewed; 282 AA.
AC P77733;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Probable formate transporter 2;
DE AltName: Full=Formate channel 2;
GN Name=focB {ECO:0000303|PubMed:9387241}; OrderedLocusNames=b2492, JW2477;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=9387241; DOI=10.1099/00221287-143-11-3633;
RA Andrews S.C., Berks B.C., McClay J., Ambler A., Quail M.A., Golby P.,
RA Guest J.R.;
RT "A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-
RT translocating formate hydrogenlyase system.";
RL Microbiology 143:3633-3647(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUBCELLULAR LOCATION, AND TOPOLOGY.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Involved in the bidirectional transport of formate.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the FNT transporter (TC 2.A.44) family.
CC {ECO:0000305}.
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DR EMBL; M63654; AAB88574.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75545.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16381.1; -; Genomic_DNA.
DR PIR; C65025; C65025.
DR RefSeq; NP_416987.1; NC_000913.3.
DR RefSeq; WP_001244734.1; NZ_LN832404.1.
DR AlphaFoldDB; P77733; -.
DR SMR; P77733; -.
DR BioGRID; 4261432; 7.
DR DIP; DIP-9671N; -.
DR IntAct; P77733; 1.
DR STRING; 511145.b2492; -.
DR TCDB; 1.A.16.1.2; the formate-nitrite transporter (fnt) family.
DR PaxDb; P77733; -.
DR PRIDE; P77733; -.
DR EnsemblBacteria; AAC75545; AAC75545; b2492.
DR EnsemblBacteria; BAA16381; BAA16381; BAA16381.
DR GeneID; 949032; -.
DR KEGG; ecj:JW2477; -.
DR KEGG; eco:b2492; -.
DR PATRIC; fig|1411691.4.peg.4247; -.
DR EchoBASE; EB3972; -.
DR eggNOG; COG2116; Bacteria.
DR HOGENOM; CLU_036896_3_0_6; -.
DR InParanoid; P77733; -.
DR OMA; WMAYGAK; -.
DR PhylomeDB; P77733; -.
DR BioCyc; EcoCyc:FOCB-MON; -.
DR BioCyc; MetaCyc:FOCB-MON; -.
DR PRO; PR:P77733; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015499; F:formate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015724; P:formate transport; IBA:GO_Central.
DR GO; GO:0019664; P:mixed acid fermentation; IMP:EcoCyc.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR000292; For/NO2_transpt.
DR InterPro; IPR024002; For/NO2_transpt_CS.
DR PANTHER; PTHR30520; PTHR30520; 1.
DR Pfam; PF01226; Form_Nir_trans; 1.
DR TIGRFAMs; TIGR00790; fnt; 1.
DR PROSITE; PS01005; FORMATE_NITRITE_TP_1; 1.
DR PROSITE; PS01006; FORMATE_NITRITE_TP_2; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..282
FT /note="Probable formate transporter 2"
FT /id="PRO_0000094721"
FT TOPO_DOM 1..35
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..68
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 90..112
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 134..163
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..190
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..248
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 270..282
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255, ECO:0000269|PubMed:15919996"
SQ SEQUENCE 282 AA; 30565 MW; 403929CDF55EDE0E CRC64;
MRNKLSFDLQ LSARKAAIAE RIAAHKIARS KVSVFLMAMS AGVFMAIGFT FYLSVIADAP
SSQALTHLVG GLCFTLGFIL LAVCGTSLFT SSVMTVMAKS RGVISWRTWL INALLVACGN
LAGIACFSLL IWFSGLVMSE NAMWGVAVLH CAEGKMHHTF TESVSLGIMC NLMVCLALWM
SYCGRSLCDK IVAMILPITL FVASGFEHCI ANLFVIPFAI AIRHFAPPPF WQLAHSSADN
FPALTVSHFI TANLLPVMLG NIIGGAVLVS MCYRAIYLRQ EP
