ID   ALAE_VIBCH              Reviewed;         150 AA.
AC   Q9KR19;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=L-alanine exporter AlaE {ECO:0000255|HAMAP-Rule:MF_00914};
GN   Name=alaE {ECO:0000255|HAMAP-Rule:MF_00914}; OrderedLocusNames=VC_1828;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: Exports L-alanine. {ECO:0000255|HAMAP-Rule:MF_00914}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00914}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00914}.
CC   -!- SIMILARITY: Belongs to the AlaE exporter family. {ECO:0000255|HAMAP-
CC       Rule:MF_00914}.
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DR   EMBL; AE003852; AAF94976.1; -; Genomic_DNA.
DR   PIR; H82150; H82150.
DR   RefSeq; NP_231462.1; NC_002505.1.
DR   RefSeq; WP_000647492.1; NZ_LT906614.1.
DR   AlphaFoldDB; Q9KR19; -.
DR   STRING; 243277.VC_1828; -.
DR   DNASU; 2613582; -.
DR   EnsemblBacteria; AAF94976; AAF94976; VC_1828.
DR   GeneID; 57740465; -.
DR   KEGG; vch:VC_1828; -.
DR   PATRIC; fig|243277.26.peg.1746; -.
DR   eggNOG; ENOG502ZRFS; Bacteria.
DR   HOGENOM; CLU_126493_0_0_6; -.
DR   OMA; ADWHQIA; -.
DR   BioCyc; VCHO:VC1828-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0034639; F:L-amino acid efflux transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032973; P:amino acid export across plasma membrane; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00914; L_Ala_exporter; 1.
DR   InterPro; IPR010574; Ala_export_AlaE.
DR   Pfam; PF06610; AlaE; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..150
FT                   /note="L-alanine exporter AlaE"
FT                   /id="PRO_0000415631"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        48..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
SQ   SEQUENCE   150 AA;  16563 MW;  945D365E591EBE22 CRC64;
     MKARGPFCIR HAAADTFAMV VFCFVTGMII EIFVSGMTFQ QSLASRTLSI PVNIAIAWPY
     GVFRDYVLRQ GRKISPTGWM KNLSDLVAYV LFQSPVYAAI LFTVGASTDQ IITAVATNAL
     VSCGMGVLYG YFLDMCRRWF KVPGYTVSEG