ID   ALAE_YERPE              Reviewed;         148 AA.
AC   Q0WJC2; Q74Q68; Q8CZQ5;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=L-alanine exporter AlaE {ECO:0000255|HAMAP-Rule:MF_00914};
GN   Name=alaE {ECO:0000255|HAMAP-Rule:MF_00914};
GN   OrderedLocusNames=YPO0544, y3636, YP_3639;
OS   Yersinia pestis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CO-92 / Biovar Orientalis;
RX   PubMed=11586360; DOI=10.1038/35097083;
RA   Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA   Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA   Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA   Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA   Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA   Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA   Stevens K., Whitehead S., Barrell B.G.;
RT   "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL   Nature 413:523-527(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KIM10+ / Biovar Mediaevalis;
RX   PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA   Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA   Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA   Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA   Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT   "Genome sequence of Yersinia pestis KIM.";
RL   J. Bacteriol. 184:4601-4611(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=91001 / Biovar Mediaevalis;
RX   PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA   Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA   Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA   Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA   Wang J., Huang P., Yang R.;
RT   "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT   avirulent to humans.";
RL   DNA Res. 11:179-197(2004).
CC   -!- FUNCTION: Exports L-alanine. {ECO:0000255|HAMAP-Rule:MF_00914}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00914}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00914}.
CC   -!- SIMILARITY: Belongs to the AlaE exporter family. {ECO:0000255|HAMAP-
CC       Rule:MF_00914}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM87184.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=AAS63787.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AL590842; CAL19224.1; -; Genomic_DNA.
DR   EMBL; AE009952; AAM87184.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AE017042; AAS63787.1; ALT_INIT; Genomic_DNA.
DR   PIR; AF0067; AF0067.
DR   RefSeq; YP_002345616.1; NC_003143.1.
DR   AlphaFoldDB; Q0WJC2; -.
DR   STRING; 214092.YPO0544; -.
DR   PaxDb; Q0WJC2; -.
DR   DNASU; 1148583; -.
DR   EnsemblBacteria; AAM87184; AAM87184; y3636.
DR   EnsemblBacteria; AAS63787; AAS63787; YP_3639.
DR   KEGG; ype:YPO0544; -.
DR   KEGG; ypk:y3636; -.
DR   KEGG; ypm:YP_3639; -.
DR   PATRIC; fig|214092.21.peg.796; -.
DR   eggNOG; ENOG502ZRFS; Bacteria.
DR   HOGENOM; CLU_126493_0_0_6; -.
DR   Proteomes; UP000000815; Chromosome.
DR   Proteomes; UP000001019; Chromosome.
DR   Proteomes; UP000002490; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0034639; F:L-amino acid efflux transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032973; P:amino acid export across plasma membrane; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00914; L_Ala_exporter; 1.
DR   InterPro; IPR010574; Ala_export_AlaE.
DR   Pfam; PF06610; AlaE; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..148
FT                   /note="L-alanine exporter AlaE"
FT                   /id="PRO_0000415634"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        49..69
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00914"
SQ   SEQUENCE   148 AA;  16746 MW;  92453DFF04FF5404 CRC64;
     MTMFSTGPRW RSAVADTFAL VVYCFVIGMA IEVVISGMTF RQSLSSRLLS IPVNILIAWP
     YGMYRDAFIR FAQRHAGQRF WARNLADLLA YVSFQSPVYA MILWSVGADF EQMISAVASN
     AVVSMVMGVA YGYFLEYCRR LFRVAGYV