ID   ALAXM_SACS2             Reviewed;         236 AA.
AC   Q97ZX2;
DT   02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Alanyl-tRNA editing protein AlaX-M;
DE            Short=AlaX-M;
DE   AltName: Full=Alanyl-tRNA deacylase AlaX-M;
GN   Name=alaXM; OrderedLocusNames=SSO0450;
OS   Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS   (Sulfolobus solfataricus).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Saccharolobus.
OX   NCBI_TaxID=273057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=11427726; DOI=10.1073/pnas.141222098;
RA   She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA   Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA   Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA   Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA   Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA   Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT   "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN   [2]
RP   FUNCTION AS TRNA(ALA) EDITING PROTEIN.
RX   PubMed=14663147; DOI=10.1073/pnas.2136934100;
RA   Ahel I., Korencic D., Ibba M., Soll D.;
RT   "Trans-editing of mischarged tRNAs.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:15422-15427(2003).
CC   -!- FUNCTION: Functions in trans to edit the amino acid moiety from
CC       incorrectly charged Ser-tRNA(Ala). {ECO:0000269|PubMed:14663147}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       Editing domain AlaX-M subfamily. {ECO:0000305}.
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DR   EMBL; AE006641; AAK40776.1; -; Genomic_DNA.
DR   PIR; A90190; A90190.
DR   RefSeq; WP_009988710.1; NC_002754.1.
DR   AlphaFoldDB; Q97ZX2; -.
DR   SMR; Q97ZX2; -.
DR   STRING; 273057.SSO0450; -.
DR   EnsemblBacteria; AAK40776; AAK40776; SSO0450.
DR   GeneID; 44129432; -.
DR   KEGG; sso:SSO0450; -.
DR   PATRIC; fig|273057.12.peg.444; -.
DR   eggNOG; arCOG01254; Archaea.
DR   HOGENOM; CLU_004485_3_2_2; -.
DR   InParanoid; Q97ZX2; -.
DR   OMA; LMRLHTA; -.
DR   PhylomeDB; Q97ZX2; -.
DR   Proteomes; UP000001974; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004813; F:alanine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IDA:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006419; P:alanyl-tRNA aminoacylation; IEA:InterPro.
DR   InterPro; IPR018165; Ala-tRNA-synth_IIc_core.
DR   InterPro; IPR018164; Ala-tRNA-synth_IIc_N.
DR   InterPro; IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   InterPro; IPR012947; tRNA_SAD.
DR   Pfam; PF01411; tRNA-synt_2c; 1.
DR   Pfam; PF07973; tRNA_SAD; 1.
DR   SMART; SM00863; tRNA_SAD; 1.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF55186; SSF55186; 1.
DR   PROSITE; PS50860; AA_TRNA_LIGASE_II_ALA; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Metal-binding; Reference proteome; Zinc.
FT   CHAIN           1..236
FT                   /note="Alanyl-tRNA editing protein AlaX-M"
FT                   /id="PRO_0000391649"
FT   BINDING         101
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         105
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         205
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   236 AA;  26710 MW;  2AFC9CF180BF368E CRC64;
     MTEELYLKDS YIKEFEGRVV RIEGNYVILD KTAFYPGGGG LDNDTGFLVN EKGERISVTE
     VKRGENGEIL HKIDQNGSLN VNEKVIGTID WDRRYRMMRL HTASHIVAAL AYRKFGALIT
     GGHISPEQAK DDFNVENKDT LIELINEANE IIKKDIELKI YFLPREEALM IPAIVKLAGR
     NPPQIPIWRI VEIPGIDIQA DGGPHVKNTK EIGEIVLLKV ENKGKGRKRV YYTVKP