ID   ALB1F_PEA               Reviewed;         130 AA.
AC   P62931; P08687; Q40999; Q7X9N3; Q9M3X4;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   31-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Albumin-1 F;
DE   AltName: Full=PA1 F;
DE   AltName: Full=PsaA1b005/PsaA1b011;
DE   Contains:
DE     RecName: Full=Albumin-1 F chain b;
DE     AltName: Full=Leginsulin F;
DE     AltName: Full=PA1b F;
DE   Contains:
DE     RecName: Full=Albumin-1 F chain a;
DE     AltName: Full=PA1a F;
DE   Flags: Precursor;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Frisson; TISSUE=Seed;
RX   AGRICOLA=IND43645431; DOI=10.1016/j.plantsci.2004.04.018;
RA   Louis S., Delobel B., Gressent F., Rahioui I., Quillien L., Vallier A.,
RA   Rahbe Y.;
RT   "Molecular and biological screening for insect-toxic seed albumins from
RT   four legume species.";
RL   Plant Sci. 167:705-714(2004).
RN   [2]
RP   PROTEIN SEQUENCE OF 27-63, AND FUNCTION.
RA   Delobel B., Grenier A., Gueguen J., Ferrasson E., Mbailao M.;
RT   "Use of a polypeptide derived from a PA1b legume albumen as insecticide.";
RL   Patent number WO9958695, 07-MAY-1999.
RN   [3]
RP   STRUCTURE BY NMR OF 27-63, AND DISULFIDE BONDS.
RX   PubMed=14556622; DOI=10.1021/bi034803l;
RA   Jouvensal L., Quillien L., Ferrasson E., Rahbe Y., Gueguen J., Vovelle F.;
RT   "PA1b, an insecticidal protein extracted from pea seeds (Pisum sativum):
RT   1H-2-D NMR study and molecular modeling.";
RL   Biochemistry 42:11915-11923(2003).
RN   [4]
RP   FUNCTION.
RX   PubMed=12755698; DOI=10.1046/j.1432-1033.2003.03611.x;
RA   Gressent F., Rahioui I., Rahbe Y.;
RT   "Characterization of a high-affinity binding site for the pea albumin 1b
RT   entomotoxin in the weevil Sitophilus.";
RL   Eur. J. Biochem. 270:2429-2435(2003).
CC   -!- FUNCTION: PA1b binds to basic 7S globulin (BG) and stimulates its
CC       phosphorylation activity. Involved in the signal transduction system to
CC       regulate the growth and differentiation as a hormone peptide (By
CC       similarity). Toxic to various insects through binding to a high
CC       affinity binding site in the insect gut. {ECO:0000250,
CC       ECO:0000269|PubMed:12755698, ECO:0000269|Ref.2}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC   -!- PTM: The C-terminal glycine may be removed from PA1b.
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DR   EMBL; AJ574793; CAE00465.1; -; mRNA.
DR   PDB; 1P8B; NMR; -; A=27-63.
DR   PDBsum; 1P8B; -.
DR   AlphaFoldDB; P62931; -.
DR   SMR; P62931; -.
DR   EvolutionaryTrace; P62931; -.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012512; Albumin_I.
DR   InterPro; IPR032000; Albumin_I_a.
DR   Pfam; PF08027; Albumin_I; 1.
DR   Pfam; PF16720; Albumin_I_a; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Knottin;
KW   Seed storage protein; Signal; Storage protein; Toxin.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000269|Ref.2"
FT   CHAIN           27..63
FT                   /note="Albumin-1 F chain b"
FT                   /id="PRO_0000032235"
FT   PROPEP          64..69
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000032236"
FT   CHAIN           70..122
FT                   /note="Albumin-1 F chain a"
FT                   /id="PRO_0000032237"
FT   PROPEP          123..130
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000032238"
FT   DISULFID        29..46
FT                   /evidence="ECO:0000269|PubMed:14556622"
FT   DISULFID        33..48
FT                   /evidence="ECO:0000269|PubMed:14556622"
FT   DISULFID        41..58
FT                   /evidence="ECO:0000269|PubMed:14556622"
FT   STRAND          30..33
FT                   /evidence="ECO:0007829|PDB:1P8B"
FT   HELIX           40..42
FT                   /evidence="ECO:0007829|PDB:1P8B"
FT   STRAND          44..49
FT                   /evidence="ECO:0007829|PDB:1P8B"
FT   STRAND          52..54
FT                   /evidence="ECO:0007829|PDB:1P8B"
FT   STRAND          56..59
FT                   /evidence="ECO:0007829|PDB:1P8B"
SQ   SEQUENCE   130 AA;  13913 MW;  FD58E2D3C99D1644 CRC64;
     MASVKLASLI VLFATLGMFL TKNVGAASCN GVCSPFEMPP CGTSACRCIP VGLVIGYCRN
     PSGVFLRTND EHPNLCESDA DCRKKGSGKF CGHYPNPGIE YGWCFASKSE AEDFFSKITQ
     KDLLKSVSTA