FOLT1_ARATH
ID FOLT1_ARATH Reviewed; 308 AA.
AC Q7XA87; Q4A3R4; Q8LFJ3; Q9FK00;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Folate transporter 1, chloroplastic;
DE Short=AtFOLT1;
GN Name=FOLT1; OrderedLocusNames=At5g66380; ORFNames=K1F13.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Wassilewskija;
RX PubMed=16055441; DOI=10.1074/jbc.m506045200;
RA Bedhomme M., Hoffmann M., McCarthy E.A., Gambonnet B., Moran R.G.,
RA Rebeille F., Ravanel S.;
RT "Folate metabolism in plants: an Arabidopsis homolog of the mammalian
RT mitochondrial folate transporter mediates folate import into
RT chloroplasts.";
RL J. Biol. Chem. 280:34823-34831(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mediates folate import into chloroplast.
CC {ECO:0000269|PubMed:16055441}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:16055441}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16055441}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:16055441}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development.
CC {ECO:0000269|PubMed:16055441}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:16055441}.
CC -!- MISCELLANEOUS: Appears to be a chloroplast envelope-located membrane
CC protein lacking an N-terminal-located transit peptide.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10914.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ852535; CAH65737.1; -; mRNA.
DR EMBL; AB013389; BAB10914.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED98206.1; -; Genomic_DNA.
DR EMBL; BT010139; AAQ22608.1; -; mRNA.
DR EMBL; AK227464; BAE99467.1; -; mRNA.
DR EMBL; AY084815; AAM61381.1; -; mRNA.
DR RefSeq; NP_569032.1; NM_126036.4.
DR AlphaFoldDB; Q7XA87; -.
DR SMR; Q7XA87; -.
DR BioGRID; 22012; 1.
DR STRING; 3702.AT5G66380.1; -.
DR TCDB; 2.A.29.10.3; the mitochondrial carrier (mc) family.
DR PaxDb; Q7XA87; -.
DR PRIDE; Q7XA87; -.
DR ProteomicsDB; 230559; -.
DR EnsemblPlants; AT5G66380.1; AT5G66380.1; AT5G66380.
DR GeneID; 836770; -.
DR Gramene; AT5G66380.1; AT5G66380.1; AT5G66380.
DR KEGG; ath:AT5G66380; -.
DR Araport; AT5G66380; -.
DR TAIR; locus:2154910; AT5G66380.
DR eggNOG; KOG0764; Eukaryota.
DR HOGENOM; CLU_015166_6_4_1; -.
DR InParanoid; Q7XA87; -.
DR OMA; GLYFLWY; -.
DR OrthoDB; 1080385at2759; -.
DR PhylomeDB; Q7XA87; -.
DR PRO; PR:Q7XA87; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q7XA87; baseline and differential.
DR Genevisible; Q7XA87; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015230; F:FAD transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008517; F:folic acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:1904947; P:folate import into mitochondrion; IBA:GO_Central.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.50.40.10; -; 2.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR InterPro; IPR044712; SLC25A32-like.
DR PANTHER; PTHR45683; PTHR45683; 1.
DR Pfam; PF00153; Mito_carr; 3.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..308
FT /note="Folate transporter 1, chloroplastic"
FT /id="PRO_0000420697"
FT TRANSMEM 10..30
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..91
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..293
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 4..94
FT /note="Solcar 1"
FT REPEAT 104..192
FT /note="Solcar 2"
FT REPEAT 213..299
FT /note="Solcar 3"
FT CONFLICT 26
FT /note="S -> P (in Ref. 1; CAH65737 and 6; AAM61381)"
FT /evidence="ECO:0000305"
FT CONFLICT 259
FT /note="I -> V (in Ref. 6; AAM61381)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 308 AA; 34119 MW; 33AB9DDA1D0488C1 CRC64;
MAASWQWENA TAGAVAGFAT VAAMHSLDVV RTRFQVNDGR GSSLPTYKNT AHAVFTIARL
EGLRGLYAGF FPAVIGSTVS WGLYFFFYGR AKQRYARGRD DEKLSPALHL ASAAEAGALV
CLCTNPIWLV KTRLQLQTPL HQTQPYSGLL DAFRTIVKEE GPRALYKGIV PGLVLVSHGA
IQFTAYEELR KIIVDLKERR RKSESTDNLL NSADYAALGG SSKVAAVLLT YPFQVIRARL
QQRPSTNGIP RYIDSLHVIR ETARYEGLRG FYRGLTANLL KNVPASSITF IVYENVLKLL
KQHPTTKD
